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Polymorphisms of methylenetetrahydrofolate reductase gene as the genetic predispositions of coronary artery diseases in eastern India
BACKGROUND: Gene–environment interaction is an important aspect in the development of coronary artery disease (CAD). The mutation (677C-T) of methylenetetrahydrofolate reductase (MTHFR) gene results in a decrease of the enzyme activity that leads to mild hyperhomocysteinemia. Elevated plasma level o...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2982204/ https://www.ncbi.nlm.nih.gov/pubmed/21187870 http://dx.doi.org/10.4103/0975-3583.70922 |
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author | Dhar, Soujatya Chatterjee, Sumana Ray, Saumitra Dutta, Anjanlal Sengupta, Bani Chakrabarti, Shila |
author_facet | Dhar, Soujatya Chatterjee, Sumana Ray, Saumitra Dutta, Anjanlal Sengupta, Bani Chakrabarti, Shila |
author_sort | Dhar, Soujatya |
collection | PubMed |
description | BACKGROUND: Gene–environment interaction is an important aspect in the development of coronary artery disease (CAD). The mutation (677C-T) of methylenetetrahydrofolate reductase (MTHFR) gene results in a decrease of the enzyme activity that leads to mild hyperhomocysteinemia. Elevated plasma level of homocysteine has been recognized as an independent risk factor for cardiovascular disease. A case–control study was designed to assess whether the prevalence of some MTHFR gene polymorphisms have any role in the development of CAD. MATERIALS AND METHODS: The study included unrelated 217 cases with CAD and 255 healthy controls. DNA was extracted from peripheral blood. MTHFR genotypes were identified by seeing the presence or absence of 677C→T mutation obtained by PCR followed by Hinf1 restriction digestion. Multiple logistic regression analysis was carried out to find association between studied genotypes and lifestyle as well as biochemical risk factors. RESULTS: The T allele was found to be associated with the disease. Significant associations were found with smoking, hypertension, diabetes, and family history of CAD. CONCLUSION: The results indicate that MTHFR 677C-T polymorphism has significant association with CADs in the population of eastern India. |
format | Text |
id | pubmed-2982204 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Medknow Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-29822042010-12-23 Polymorphisms of methylenetetrahydrofolate reductase gene as the genetic predispositions of coronary artery diseases in eastern India Dhar, Soujatya Chatterjee, Sumana Ray, Saumitra Dutta, Anjanlal Sengupta, Bani Chakrabarti, Shila J Cardiovasc Dis Res Original Paper BACKGROUND: Gene–environment interaction is an important aspect in the development of coronary artery disease (CAD). The mutation (677C-T) of methylenetetrahydrofolate reductase (MTHFR) gene results in a decrease of the enzyme activity that leads to mild hyperhomocysteinemia. Elevated plasma level of homocysteine has been recognized as an independent risk factor for cardiovascular disease. A case–control study was designed to assess whether the prevalence of some MTHFR gene polymorphisms have any role in the development of CAD. MATERIALS AND METHODS: The study included unrelated 217 cases with CAD and 255 healthy controls. DNA was extracted from peripheral blood. MTHFR genotypes were identified by seeing the presence or absence of 677C→T mutation obtained by PCR followed by Hinf1 restriction digestion. Multiple logistic regression analysis was carried out to find association between studied genotypes and lifestyle as well as biochemical risk factors. RESULTS: The T allele was found to be associated with the disease. Significant associations were found with smoking, hypertension, diabetes, and family history of CAD. CONCLUSION: The results indicate that MTHFR 677C-T polymorphism has significant association with CADs in the population of eastern India. Medknow Publications 2010 /pmc/articles/PMC2982204/ /pubmed/21187870 http://dx.doi.org/10.4103/0975-3583.70922 Text en © Journal of Cardiovascular Disease Research http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Paper Dhar, Soujatya Chatterjee, Sumana Ray, Saumitra Dutta, Anjanlal Sengupta, Bani Chakrabarti, Shila Polymorphisms of methylenetetrahydrofolate reductase gene as the genetic predispositions of coronary artery diseases in eastern India |
title | Polymorphisms of methylenetetrahydrofolate reductase gene as the genetic predispositions of coronary artery diseases in eastern India |
title_full | Polymorphisms of methylenetetrahydrofolate reductase gene as the genetic predispositions of coronary artery diseases in eastern India |
title_fullStr | Polymorphisms of methylenetetrahydrofolate reductase gene as the genetic predispositions of coronary artery diseases in eastern India |
title_full_unstemmed | Polymorphisms of methylenetetrahydrofolate reductase gene as the genetic predispositions of coronary artery diseases in eastern India |
title_short | Polymorphisms of methylenetetrahydrofolate reductase gene as the genetic predispositions of coronary artery diseases in eastern India |
title_sort | polymorphisms of methylenetetrahydrofolate reductase gene as the genetic predispositions of coronary artery diseases in eastern india |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2982204/ https://www.ncbi.nlm.nih.gov/pubmed/21187870 http://dx.doi.org/10.4103/0975-3583.70922 |
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