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Generation of representative primary virus isolates from blood plasma after isolation of HIV-1 with CD44 MicroBeads
Infection of cell cultures with cell-free virus isolated from HIV-infected patients is notoriously difficult and results in a loss of viral variation. Here, we describe viral sequences from PBMC, U87.CD4.CCR5 and U87.CD4.CXCR4 cell cultures and compare them to those from blood plasma from 12 patient...
Autores principales: | Cornelissen, Marion, Heeregrave, Edwin J., Zorgdrager, Fokla, Pollakis, Georgios, Paxton, William A., van der Kuyl, Antoinette C. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Springer Vienna
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2982953/ https://www.ncbi.nlm.nih.gov/pubmed/20865288 http://dx.doi.org/10.1007/s00705-010-0806-x |
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