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Flotillin microdomains interact with the cortical cytoskeleton to control uropod formation and neutrophil recruitment

We studied the function of plasma membrane microdomains defined by the proteins flotillin 1 and flotillin 2 in uropod formation and neutrophil chemotaxis. Flotillins become concentrated in the uropod of neutrophils after exposure to chemoattractants such as N-formyl-Met-Leu-Phe (fMLP). Here, we show...

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Autores principales: Ludwig, Alexander, Otto, Grant P., Riento, Kirsi, Hams, Emily, Fallon, Padraic G., Nichols, Ben J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2983060/
https://www.ncbi.nlm.nih.gov/pubmed/21059848
http://dx.doi.org/10.1083/jcb.201005140
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author Ludwig, Alexander
Otto, Grant P.
Riento, Kirsi
Hams, Emily
Fallon, Padraic G.
Nichols, Ben J.
author_facet Ludwig, Alexander
Otto, Grant P.
Riento, Kirsi
Hams, Emily
Fallon, Padraic G.
Nichols, Ben J.
author_sort Ludwig, Alexander
collection PubMed
description We studied the function of plasma membrane microdomains defined by the proteins flotillin 1 and flotillin 2 in uropod formation and neutrophil chemotaxis. Flotillins become concentrated in the uropod of neutrophils after exposure to chemoattractants such as N-formyl-Met-Leu-Phe (fMLP). Here, we show that mice lacking flotillin 1 do not have flotillin microdomains, and that recruitment of neutrophils toward fMLP in vivo is reduced in these mice. Ex vivo, migration of neutrophils through a resistive matrix is reduced in the absence of flotillin microdomains, but the machinery required for sensing chemoattractant functions normally. Flotillin microdomains specifically associate with myosin IIa, and spectrins. Both uropod formation and myosin IIa activity are compromised in flotillin 1 knockout neutrophils. We conclude that the association between flotillin microdomains and cortical cytoskeleton has important functions during neutrophil migration, in uropod formation, and in the regulation of myosin IIa.
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spelling pubmed-29830602011-05-15 Flotillin microdomains interact with the cortical cytoskeleton to control uropod formation and neutrophil recruitment Ludwig, Alexander Otto, Grant P. Riento, Kirsi Hams, Emily Fallon, Padraic G. Nichols, Ben J. J Cell Biol Research Articles We studied the function of plasma membrane microdomains defined by the proteins flotillin 1 and flotillin 2 in uropod formation and neutrophil chemotaxis. Flotillins become concentrated in the uropod of neutrophils after exposure to chemoattractants such as N-formyl-Met-Leu-Phe (fMLP). Here, we show that mice lacking flotillin 1 do not have flotillin microdomains, and that recruitment of neutrophils toward fMLP in vivo is reduced in these mice. Ex vivo, migration of neutrophils through a resistive matrix is reduced in the absence of flotillin microdomains, but the machinery required for sensing chemoattractant functions normally. Flotillin microdomains specifically associate with myosin IIa, and spectrins. Both uropod formation and myosin IIa activity are compromised in flotillin 1 knockout neutrophils. We conclude that the association between flotillin microdomains and cortical cytoskeleton has important functions during neutrophil migration, in uropod formation, and in the regulation of myosin IIa. The Rockefeller University Press 2010-11-15 /pmc/articles/PMC2983060/ /pubmed/21059848 http://dx.doi.org/10.1083/jcb.201005140 Text en © 2010 Ludwig et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Ludwig, Alexander
Otto, Grant P.
Riento, Kirsi
Hams, Emily
Fallon, Padraic G.
Nichols, Ben J.
Flotillin microdomains interact with the cortical cytoskeleton to control uropod formation and neutrophil recruitment
title Flotillin microdomains interact with the cortical cytoskeleton to control uropod formation and neutrophil recruitment
title_full Flotillin microdomains interact with the cortical cytoskeleton to control uropod formation and neutrophil recruitment
title_fullStr Flotillin microdomains interact with the cortical cytoskeleton to control uropod formation and neutrophil recruitment
title_full_unstemmed Flotillin microdomains interact with the cortical cytoskeleton to control uropod formation and neutrophil recruitment
title_short Flotillin microdomains interact with the cortical cytoskeleton to control uropod formation and neutrophil recruitment
title_sort flotillin microdomains interact with the cortical cytoskeleton to control uropod formation and neutrophil recruitment
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2983060/
https://www.ncbi.nlm.nih.gov/pubmed/21059848
http://dx.doi.org/10.1083/jcb.201005140
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