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Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission

Cytokinesis requires a membrane-remodeling and fission event termed abscission that occurs after chromosome segregation, cleavage furrow formation, and contraction have completed. In this study, we show how abscission factor recruitment is controlled by the Polo-like kinase 1 (Plk1). At the metaphas...

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Detalles Bibliográficos
Autores principales: Bastos, Ricardo Nunes, Barr, Francis A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2983065/
https://www.ncbi.nlm.nih.gov/pubmed/21079244
http://dx.doi.org/10.1083/jcb.201008108
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author Bastos, Ricardo Nunes
Barr, Francis A.
author_facet Bastos, Ricardo Nunes
Barr, Francis A.
author_sort Bastos, Ricardo Nunes
collection PubMed
description Cytokinesis requires a membrane-remodeling and fission event termed abscission that occurs after chromosome segregation, cleavage furrow formation, and contraction have completed. In this study, we show how abscission factor recruitment is controlled by the Polo-like kinase 1 (Plk1). At the metaphase–anaphase transition, Plk1 initiates cleavage furrow formation and is then progressively degraded during mitotic exit. During this period, Plk1 phosphorylates the abscission factor Cep55 in trans and prevents its untimely recruitment to the anaphase spindle. A Plk1 phosphorylation site mutant of Cep55 is prematurely recruited to the anaphase spindle and fails to support abscission. Endogenous Cep55 behaves similarly after Plk1 inhibition by the drugs BI2536 or GW842862. Only once Plk1 is degraded can Cep55 target to the midbody and promote abscission. Blocking Plk1 degradation leads to elevated levels of Plk1 at the midbody and the failure of Cep55 recruitment. Thus, Plk1 activity negatively regulates Cep55 to ensure orderly abscission factor recruitment and ensures that this occurs only once cell contraction has completed.
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spelling pubmed-29830652011-05-15 Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission Bastos, Ricardo Nunes Barr, Francis A. J Cell Biol Research Articles Cytokinesis requires a membrane-remodeling and fission event termed abscission that occurs after chromosome segregation, cleavage furrow formation, and contraction have completed. In this study, we show how abscission factor recruitment is controlled by the Polo-like kinase 1 (Plk1). At the metaphase–anaphase transition, Plk1 initiates cleavage furrow formation and is then progressively degraded during mitotic exit. During this period, Plk1 phosphorylates the abscission factor Cep55 in trans and prevents its untimely recruitment to the anaphase spindle. A Plk1 phosphorylation site mutant of Cep55 is prematurely recruited to the anaphase spindle and fails to support abscission. Endogenous Cep55 behaves similarly after Plk1 inhibition by the drugs BI2536 or GW842862. Only once Plk1 is degraded can Cep55 target to the midbody and promote abscission. Blocking Plk1 degradation leads to elevated levels of Plk1 at the midbody and the failure of Cep55 recruitment. Thus, Plk1 activity negatively regulates Cep55 to ensure orderly abscission factor recruitment and ensures that this occurs only once cell contraction has completed. The Rockefeller University Press 2010-11-15 /pmc/articles/PMC2983065/ /pubmed/21079244 http://dx.doi.org/10.1083/jcb.201008108 Text en © 2010 Bastos and Barr This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Bastos, Ricardo Nunes
Barr, Francis A.
Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission
title Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission
title_full Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission
title_fullStr Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission
title_full_unstemmed Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission
title_short Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission
title_sort plk1 negatively regulates cep55 recruitment to the midbody to ensure orderly abscission
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2983065/
https://www.ncbi.nlm.nih.gov/pubmed/21079244
http://dx.doi.org/10.1083/jcb.201008108
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