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Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission
Cytokinesis requires a membrane-remodeling and fission event termed abscission that occurs after chromosome segregation, cleavage furrow formation, and contraction have completed. In this study, we show how abscission factor recruitment is controlled by the Polo-like kinase 1 (Plk1). At the metaphas...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2983065/ https://www.ncbi.nlm.nih.gov/pubmed/21079244 http://dx.doi.org/10.1083/jcb.201008108 |
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author | Bastos, Ricardo Nunes Barr, Francis A. |
author_facet | Bastos, Ricardo Nunes Barr, Francis A. |
author_sort | Bastos, Ricardo Nunes |
collection | PubMed |
description | Cytokinesis requires a membrane-remodeling and fission event termed abscission that occurs after chromosome segregation, cleavage furrow formation, and contraction have completed. In this study, we show how abscission factor recruitment is controlled by the Polo-like kinase 1 (Plk1). At the metaphase–anaphase transition, Plk1 initiates cleavage furrow formation and is then progressively degraded during mitotic exit. During this period, Plk1 phosphorylates the abscission factor Cep55 in trans and prevents its untimely recruitment to the anaphase spindle. A Plk1 phosphorylation site mutant of Cep55 is prematurely recruited to the anaphase spindle and fails to support abscission. Endogenous Cep55 behaves similarly after Plk1 inhibition by the drugs BI2536 or GW842862. Only once Plk1 is degraded can Cep55 target to the midbody and promote abscission. Blocking Plk1 degradation leads to elevated levels of Plk1 at the midbody and the failure of Cep55 recruitment. Thus, Plk1 activity negatively regulates Cep55 to ensure orderly abscission factor recruitment and ensures that this occurs only once cell contraction has completed. |
format | Text |
id | pubmed-2983065 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-29830652011-05-15 Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission Bastos, Ricardo Nunes Barr, Francis A. J Cell Biol Research Articles Cytokinesis requires a membrane-remodeling and fission event termed abscission that occurs after chromosome segregation, cleavage furrow formation, and contraction have completed. In this study, we show how abscission factor recruitment is controlled by the Polo-like kinase 1 (Plk1). At the metaphase–anaphase transition, Plk1 initiates cleavage furrow formation and is then progressively degraded during mitotic exit. During this period, Plk1 phosphorylates the abscission factor Cep55 in trans and prevents its untimely recruitment to the anaphase spindle. A Plk1 phosphorylation site mutant of Cep55 is prematurely recruited to the anaphase spindle and fails to support abscission. Endogenous Cep55 behaves similarly after Plk1 inhibition by the drugs BI2536 or GW842862. Only once Plk1 is degraded can Cep55 target to the midbody and promote abscission. Blocking Plk1 degradation leads to elevated levels of Plk1 at the midbody and the failure of Cep55 recruitment. Thus, Plk1 activity negatively regulates Cep55 to ensure orderly abscission factor recruitment and ensures that this occurs only once cell contraction has completed. The Rockefeller University Press 2010-11-15 /pmc/articles/PMC2983065/ /pubmed/21079244 http://dx.doi.org/10.1083/jcb.201008108 Text en © 2010 Bastos and Barr This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Bastos, Ricardo Nunes Barr, Francis A. Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission |
title | Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission |
title_full | Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission |
title_fullStr | Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission |
title_full_unstemmed | Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission |
title_short | Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission |
title_sort | plk1 negatively regulates cep55 recruitment to the midbody to ensure orderly abscission |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2983065/ https://www.ncbi.nlm.nih.gov/pubmed/21079244 http://dx.doi.org/10.1083/jcb.201008108 |
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