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Cytoskeletal mechanics of proplatelet maturation and platelet release
Megakaryocytes generate platelets by remodeling their cytoplasm into long proplatelet extensions, which serve as assembly lines for platelet production. Although the mechanics of proplatelet elongation have been studied, the terminal steps of proplatelet maturation and platelet release remain poorly...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2983072/ https://www.ncbi.nlm.nih.gov/pubmed/21079248 http://dx.doi.org/10.1083/jcb.201006102 |
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author | Thon, Jonathan N. Montalvo, Alejandro Patel-Hett, Sunita Devine, Matthew T. Richardson, Jennifer L. Ehrlicher, Allen Larson, Mark K. Hoffmeister, Karin Hartwig, John H. Italiano, Joseph E. |
author_facet | Thon, Jonathan N. Montalvo, Alejandro Patel-Hett, Sunita Devine, Matthew T. Richardson, Jennifer L. Ehrlicher, Allen Larson, Mark K. Hoffmeister, Karin Hartwig, John H. Italiano, Joseph E. |
author_sort | Thon, Jonathan N. |
collection | PubMed |
description | Megakaryocytes generate platelets by remodeling their cytoplasm into long proplatelet extensions, which serve as assembly lines for platelet production. Although the mechanics of proplatelet elongation have been studied, the terminal steps of proplatelet maturation and platelet release remain poorly understood. To elucidate this process, released proplatelets were isolated, and their conversion into individual platelets was assessed. This enabled us to (a) define and quantify the different stages in platelet maturation, (b) identify a new intermediate stage in platelet production, the preplatelet, (c) delineate the cytoskeletal mechanics involved in preplatelet/proplatelet interconversion, and (d) model proplatelet fission and platelet release. Preplatelets are anucleate discoid particles 2–10 µm across that have the capacity to convert reversibly into elongated proplatelets by twisting microtubule-based forces that can be visualized in proplatelets expressing GFP–β1-tubulin. The release of platelets from the ends of proplatelets occurs at an increasing rate in time during culture, as larger proplatelets undergo successive fission, and is potentiated by shear. |
format | Text |
id | pubmed-2983072 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-29830722011-05-15 Cytoskeletal mechanics of proplatelet maturation and platelet release Thon, Jonathan N. Montalvo, Alejandro Patel-Hett, Sunita Devine, Matthew T. Richardson, Jennifer L. Ehrlicher, Allen Larson, Mark K. Hoffmeister, Karin Hartwig, John H. Italiano, Joseph E. J Cell Biol Research Articles Megakaryocytes generate platelets by remodeling their cytoplasm into long proplatelet extensions, which serve as assembly lines for platelet production. Although the mechanics of proplatelet elongation have been studied, the terminal steps of proplatelet maturation and platelet release remain poorly understood. To elucidate this process, released proplatelets were isolated, and their conversion into individual platelets was assessed. This enabled us to (a) define and quantify the different stages in platelet maturation, (b) identify a new intermediate stage in platelet production, the preplatelet, (c) delineate the cytoskeletal mechanics involved in preplatelet/proplatelet interconversion, and (d) model proplatelet fission and platelet release. Preplatelets are anucleate discoid particles 2–10 µm across that have the capacity to convert reversibly into elongated proplatelets by twisting microtubule-based forces that can be visualized in proplatelets expressing GFP–β1-tubulin. The release of platelets from the ends of proplatelets occurs at an increasing rate in time during culture, as larger proplatelets undergo successive fission, and is potentiated by shear. The Rockefeller University Press 2010-11-15 /pmc/articles/PMC2983072/ /pubmed/21079248 http://dx.doi.org/10.1083/jcb.201006102 Text en © 2010 Thon et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Thon, Jonathan N. Montalvo, Alejandro Patel-Hett, Sunita Devine, Matthew T. Richardson, Jennifer L. Ehrlicher, Allen Larson, Mark K. Hoffmeister, Karin Hartwig, John H. Italiano, Joseph E. Cytoskeletal mechanics of proplatelet maturation and platelet release |
title | Cytoskeletal mechanics of proplatelet maturation and platelet release |
title_full | Cytoskeletal mechanics of proplatelet maturation and platelet release |
title_fullStr | Cytoskeletal mechanics of proplatelet maturation and platelet release |
title_full_unstemmed | Cytoskeletal mechanics of proplatelet maturation and platelet release |
title_short | Cytoskeletal mechanics of proplatelet maturation and platelet release |
title_sort | cytoskeletal mechanics of proplatelet maturation and platelet release |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2983072/ https://www.ncbi.nlm.nih.gov/pubmed/21079248 http://dx.doi.org/10.1083/jcb.201006102 |
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