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Cytoskeletal mechanics of proplatelet maturation and platelet release

Megakaryocytes generate platelets by remodeling their cytoplasm into long proplatelet extensions, which serve as assembly lines for platelet production. Although the mechanics of proplatelet elongation have been studied, the terminal steps of proplatelet maturation and platelet release remain poorly...

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Autores principales: Thon, Jonathan N., Montalvo, Alejandro, Patel-Hett, Sunita, Devine, Matthew T., Richardson, Jennifer L., Ehrlicher, Allen, Larson, Mark K., Hoffmeister, Karin, Hartwig, John H., Italiano, Joseph E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2983072/
https://www.ncbi.nlm.nih.gov/pubmed/21079248
http://dx.doi.org/10.1083/jcb.201006102
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author Thon, Jonathan N.
Montalvo, Alejandro
Patel-Hett, Sunita
Devine, Matthew T.
Richardson, Jennifer L.
Ehrlicher, Allen
Larson, Mark K.
Hoffmeister, Karin
Hartwig, John H.
Italiano, Joseph E.
author_facet Thon, Jonathan N.
Montalvo, Alejandro
Patel-Hett, Sunita
Devine, Matthew T.
Richardson, Jennifer L.
Ehrlicher, Allen
Larson, Mark K.
Hoffmeister, Karin
Hartwig, John H.
Italiano, Joseph E.
author_sort Thon, Jonathan N.
collection PubMed
description Megakaryocytes generate platelets by remodeling their cytoplasm into long proplatelet extensions, which serve as assembly lines for platelet production. Although the mechanics of proplatelet elongation have been studied, the terminal steps of proplatelet maturation and platelet release remain poorly understood. To elucidate this process, released proplatelets were isolated, and their conversion into individual platelets was assessed. This enabled us to (a) define and quantify the different stages in platelet maturation, (b) identify a new intermediate stage in platelet production, the preplatelet, (c) delineate the cytoskeletal mechanics involved in preplatelet/proplatelet interconversion, and (d) model proplatelet fission and platelet release. Preplatelets are anucleate discoid particles 2–10 µm across that have the capacity to convert reversibly into elongated proplatelets by twisting microtubule-based forces that can be visualized in proplatelets expressing GFP–β1-tubulin. The release of platelets from the ends of proplatelets occurs at an increasing rate in time during culture, as larger proplatelets undergo successive fission, and is potentiated by shear.
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spelling pubmed-29830722011-05-15 Cytoskeletal mechanics of proplatelet maturation and platelet release Thon, Jonathan N. Montalvo, Alejandro Patel-Hett, Sunita Devine, Matthew T. Richardson, Jennifer L. Ehrlicher, Allen Larson, Mark K. Hoffmeister, Karin Hartwig, John H. Italiano, Joseph E. J Cell Biol Research Articles Megakaryocytes generate platelets by remodeling their cytoplasm into long proplatelet extensions, which serve as assembly lines for platelet production. Although the mechanics of proplatelet elongation have been studied, the terminal steps of proplatelet maturation and platelet release remain poorly understood. To elucidate this process, released proplatelets were isolated, and their conversion into individual platelets was assessed. This enabled us to (a) define and quantify the different stages in platelet maturation, (b) identify a new intermediate stage in platelet production, the preplatelet, (c) delineate the cytoskeletal mechanics involved in preplatelet/proplatelet interconversion, and (d) model proplatelet fission and platelet release. Preplatelets are anucleate discoid particles 2–10 µm across that have the capacity to convert reversibly into elongated proplatelets by twisting microtubule-based forces that can be visualized in proplatelets expressing GFP–β1-tubulin. The release of platelets from the ends of proplatelets occurs at an increasing rate in time during culture, as larger proplatelets undergo successive fission, and is potentiated by shear. The Rockefeller University Press 2010-11-15 /pmc/articles/PMC2983072/ /pubmed/21079248 http://dx.doi.org/10.1083/jcb.201006102 Text en © 2010 Thon et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Thon, Jonathan N.
Montalvo, Alejandro
Patel-Hett, Sunita
Devine, Matthew T.
Richardson, Jennifer L.
Ehrlicher, Allen
Larson, Mark K.
Hoffmeister, Karin
Hartwig, John H.
Italiano, Joseph E.
Cytoskeletal mechanics of proplatelet maturation and platelet release
title Cytoskeletal mechanics of proplatelet maturation and platelet release
title_full Cytoskeletal mechanics of proplatelet maturation and platelet release
title_fullStr Cytoskeletal mechanics of proplatelet maturation and platelet release
title_full_unstemmed Cytoskeletal mechanics of proplatelet maturation and platelet release
title_short Cytoskeletal mechanics of proplatelet maturation and platelet release
title_sort cytoskeletal mechanics of proplatelet maturation and platelet release
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2983072/
https://www.ncbi.nlm.nih.gov/pubmed/21079248
http://dx.doi.org/10.1083/jcb.201006102
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