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Pharmacokinetics of cytisine after single intravenous and oral administration in rabbits

The aim of this study is to develop a sensitive HPLC method for the quantitative determination of cytisine in serum and to characterize the pharmacokinetic behaviour of cytisine after oral and intravenous administration in rabbits. The pharmacokinetic behaviour of cytisine is studied in male and fem...

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Autores principales: Astroug, Henri, Simeonova, Roumiana, Kassabova, Lilia V., Danchev, Nikolay, Svinarov, Dobrin
Formato: Texto
Lenguaje:English
Publicado: Slovak Toxicology Society SETOX 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2984118/
https://www.ncbi.nlm.nih.gov/pubmed/21217866
http://dx.doi.org/10.2478/v10102-010-0003-5
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author Astroug, Henri
Simeonova, Roumiana
Kassabova, Lilia V.
Danchev, Nikolay
Svinarov, Dobrin
author_facet Astroug, Henri
Simeonova, Roumiana
Kassabova, Lilia V.
Danchev, Nikolay
Svinarov, Dobrin
author_sort Astroug, Henri
collection PubMed
description The aim of this study is to develop a sensitive HPLC method for the quantitative determination of cytisine in serum and to characterize the pharmacokinetic behaviour of cytisine after oral and intravenous administration in rabbits. The pharmacokinetic behaviour of cytisine is studied in male and female New Zealand rabbits after oral and intravenous administration. Cytisine is administered orally (dose of 5 mg/kg b.w.) under fasting condition (12 hours) and intravenously (dose 1 mg/kg b.w.) in the marginal ear vein. Cytisine serum concentrations are measured using a highly selective and sensitive validated HPLC method with UV detection. Linearity of the method is in the range 12–2 400 µg/L; accuracy and precision are both within ± 10%, and the limit of detection is 4 µg/L. Selectivity and stability are also validated. Basic pharmacokinetic parameters of cytisine after single oral and intravenous administration are calculated using TOPFIT software. Pharmacokinetic analysis suggests a rapid but incomplete absorption of cytisine after oral administration.
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spelling pubmed-29841182011-01-07 Pharmacokinetics of cytisine after single intravenous and oral administration in rabbits Astroug, Henri Simeonova, Roumiana Kassabova, Lilia V. Danchev, Nikolay Svinarov, Dobrin Interdiscip Toxicol Original Article The aim of this study is to develop a sensitive HPLC method for the quantitative determination of cytisine in serum and to characterize the pharmacokinetic behaviour of cytisine after oral and intravenous administration in rabbits. The pharmacokinetic behaviour of cytisine is studied in male and female New Zealand rabbits after oral and intravenous administration. Cytisine is administered orally (dose of 5 mg/kg b.w.) under fasting condition (12 hours) and intravenously (dose 1 mg/kg b.w.) in the marginal ear vein. Cytisine serum concentrations are measured using a highly selective and sensitive validated HPLC method with UV detection. Linearity of the method is in the range 12–2 400 µg/L; accuracy and precision are both within ± 10%, and the limit of detection is 4 µg/L. Selectivity and stability are also validated. Basic pharmacokinetic parameters of cytisine after single oral and intravenous administration are calculated using TOPFIT software. Pharmacokinetic analysis suggests a rapid but incomplete absorption of cytisine after oral administration. Slovak Toxicology Society SETOX 2010-03 2010-03-29 /pmc/articles/PMC2984118/ /pubmed/21217866 http://dx.doi.org/10.2478/v10102-010-0003-5 Text en Copyright©2010 Slovak Toxicology Society SETOX http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Astroug, Henri
Simeonova, Roumiana
Kassabova, Lilia V.
Danchev, Nikolay
Svinarov, Dobrin
Pharmacokinetics of cytisine after single intravenous and oral administration in rabbits
title Pharmacokinetics of cytisine after single intravenous and oral administration in rabbits
title_full Pharmacokinetics of cytisine after single intravenous and oral administration in rabbits
title_fullStr Pharmacokinetics of cytisine after single intravenous and oral administration in rabbits
title_full_unstemmed Pharmacokinetics of cytisine after single intravenous and oral administration in rabbits
title_short Pharmacokinetics of cytisine after single intravenous and oral administration in rabbits
title_sort pharmacokinetics of cytisine after single intravenous and oral administration in rabbits
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2984118/
https://www.ncbi.nlm.nih.gov/pubmed/21217866
http://dx.doi.org/10.2478/v10102-010-0003-5
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