Cargando…
Spatio-temporal expression of a novel neuron-derived neurotrophic factor (NDNF) in mouse brains during development
BACKGROUND: Neuron-derived neurotrophic factor (NDNF) is evolutionarily well conserved, being present in invertebrate animals such as the nematode, Caenorhabditis elegans, as well as in the fruit fly, Drosophila melanogaster. Multiple cysteines are conserved between species and secondary structure p...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2984559/ https://www.ncbi.nlm.nih.gov/pubmed/20969804 http://dx.doi.org/10.1186/1471-2202-11-137 |
_version_ | 1782192114126290944 |
---|---|
author | Kuang, Xiu-Li Zhao, Xiao-Mei Xu, Hai-Fang Shi, Yuan-Yuan Deng, Jin-Bo Sun, Guo-Tao |
author_facet | Kuang, Xiu-Li Zhao, Xiao-Mei Xu, Hai-Fang Shi, Yuan-Yuan Deng, Jin-Bo Sun, Guo-Tao |
author_sort | Kuang, Xiu-Li |
collection | PubMed |
description | BACKGROUND: Neuron-derived neurotrophic factor (NDNF) is evolutionarily well conserved, being present in invertebrate animals such as the nematode, Caenorhabditis elegans, as well as in the fruit fly, Drosophila melanogaster. Multiple cysteines are conserved between species and secondary structure prediction shows that NDNF is mainly composed of beta-strands. In this study, we aimed to investigate the function of NDNF. RESULTS: NDNF is a glycosylated, disulfide-bonded secretory protein that contains a fibronectin type III domain. NDNF promoted migration and growth and elicited neurite outgrowth of mouse hippocampal neurons in culture. NDNF also protected cultured hippocamal neurons against excitotoxicity and amyloid beta-peptide toxicity. Western blotting showed that NDNF was exclusively expressed in the brain and spinal cord. Immunostaining indicated that NDNF was expressed by neurons and not by astrocytes. Cajal-Retzius cells, cortex neurons, hippocampus neurons, olfactory mitral cells, cerebellar purkinje cells, cerebellar granular cells and spinal neurons were found to be NDNF-positive. NDNF expression was observed in the neurons during development. CONCLUSIONS: The results of this study indicated that NDNF is a novel neurotrophic factor derived from neurons that may be useful in the treatment of neuronal degeneration diseases and nerve injuries. |
format | Text |
id | pubmed-2984559 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-29845592010-11-19 Spatio-temporal expression of a novel neuron-derived neurotrophic factor (NDNF) in mouse brains during development Kuang, Xiu-Li Zhao, Xiao-Mei Xu, Hai-Fang Shi, Yuan-Yuan Deng, Jin-Bo Sun, Guo-Tao BMC Neurosci Research Article BACKGROUND: Neuron-derived neurotrophic factor (NDNF) is evolutionarily well conserved, being present in invertebrate animals such as the nematode, Caenorhabditis elegans, as well as in the fruit fly, Drosophila melanogaster. Multiple cysteines are conserved between species and secondary structure prediction shows that NDNF is mainly composed of beta-strands. In this study, we aimed to investigate the function of NDNF. RESULTS: NDNF is a glycosylated, disulfide-bonded secretory protein that contains a fibronectin type III domain. NDNF promoted migration and growth and elicited neurite outgrowth of mouse hippocampal neurons in culture. NDNF also protected cultured hippocamal neurons against excitotoxicity and amyloid beta-peptide toxicity. Western blotting showed that NDNF was exclusively expressed in the brain and spinal cord. Immunostaining indicated that NDNF was expressed by neurons and not by astrocytes. Cajal-Retzius cells, cortex neurons, hippocampus neurons, olfactory mitral cells, cerebellar purkinje cells, cerebellar granular cells and spinal neurons were found to be NDNF-positive. NDNF expression was observed in the neurons during development. CONCLUSIONS: The results of this study indicated that NDNF is a novel neurotrophic factor derived from neurons that may be useful in the treatment of neuronal degeneration diseases and nerve injuries. BioMed Central 2010-10-25 /pmc/articles/PMC2984559/ /pubmed/20969804 http://dx.doi.org/10.1186/1471-2202-11-137 Text en Copyright ©2010 Kuang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kuang, Xiu-Li Zhao, Xiao-Mei Xu, Hai-Fang Shi, Yuan-Yuan Deng, Jin-Bo Sun, Guo-Tao Spatio-temporal expression of a novel neuron-derived neurotrophic factor (NDNF) in mouse brains during development |
title | Spatio-temporal expression of a novel neuron-derived neurotrophic factor (NDNF) in mouse brains during development |
title_full | Spatio-temporal expression of a novel neuron-derived neurotrophic factor (NDNF) in mouse brains during development |
title_fullStr | Spatio-temporal expression of a novel neuron-derived neurotrophic factor (NDNF) in mouse brains during development |
title_full_unstemmed | Spatio-temporal expression of a novel neuron-derived neurotrophic factor (NDNF) in mouse brains during development |
title_short | Spatio-temporal expression of a novel neuron-derived neurotrophic factor (NDNF) in mouse brains during development |
title_sort | spatio-temporal expression of a novel neuron-derived neurotrophic factor (ndnf) in mouse brains during development |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2984559/ https://www.ncbi.nlm.nih.gov/pubmed/20969804 http://dx.doi.org/10.1186/1471-2202-11-137 |
work_keys_str_mv | AT kuangxiuli spatiotemporalexpressionofanovelneuronderivedneurotrophicfactorndnfinmousebrainsduringdevelopment AT zhaoxiaomei spatiotemporalexpressionofanovelneuronderivedneurotrophicfactorndnfinmousebrainsduringdevelopment AT xuhaifang spatiotemporalexpressionofanovelneuronderivedneurotrophicfactorndnfinmousebrainsduringdevelopment AT shiyuanyuan spatiotemporalexpressionofanovelneuronderivedneurotrophicfactorndnfinmousebrainsduringdevelopment AT dengjinbo spatiotemporalexpressionofanovelneuronderivedneurotrophicfactorndnfinmousebrainsduringdevelopment AT sunguotao spatiotemporalexpressionofanovelneuronderivedneurotrophicfactorndnfinmousebrainsduringdevelopment |