Cargando…
The Agaricus bisporus cox1 Gene: The Longest Mitochondrial Gene and the Largest Reservoir of Mitochondrial Group I Introns
In eukaryotes, introns are located in nuclear and organelle genes from several kingdoms. Large introns (up to 5 kbp) are frequent in mitochondrial genomes of plant and fungi but scarce in Metazoa, even if these organisms are grouped with fungi among the Opisthokonts. Mitochondrial introns are classi...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2987802/ https://www.ncbi.nlm.nih.gov/pubmed/21124976 http://dx.doi.org/10.1371/journal.pone.0014048 |
_version_ | 1782192157009903616 |
---|---|
author | Férandon, Cyril Moukha, Serge Callac, Philippe Benedetto, Jean-Pierre Castroviejo, Michel Barroso, Gérard |
author_facet | Férandon, Cyril Moukha, Serge Callac, Philippe Benedetto, Jean-Pierre Castroviejo, Michel Barroso, Gérard |
author_sort | Férandon, Cyril |
collection | PubMed |
description | In eukaryotes, introns are located in nuclear and organelle genes from several kingdoms. Large introns (up to 5 kbp) are frequent in mitochondrial genomes of plant and fungi but scarce in Metazoa, even if these organisms are grouped with fungi among the Opisthokonts. Mitochondrial introns are classified in two groups (I and II) according to their RNA secondary structure involved in the intron self-splicing mechanism. Most of these mitochondrial group I introns carry a “Homing Endonuclease Gene” (heg) encoding a DNA endonuclease acting in transfer and site-specific integration (“homing”) and allowing intron spreading and gain after lateral transfer even between species from different kingdoms. Opposed to this gain mechanism, is another which implies that introns, which would have been abundant in the ancestral genes, would mainly evolve by loss. The importance of both mechanisms (loss and gain) is matter of debate. Here we report the sequence of the cox1 gene of the button mushroom Agaricus bisporus, the most widely cultivated mushroom in the world. This gene is both the longest mitochondrial gene (29,902 nt) and the largest group I intron reservoir reported to date with 18 group I and 1 group II. An exhaustive analysis of the group I introns available in cox1 genes shows that they are mobile genetic elements whose numerous events of loss and gain by lateral transfer combine to explain their wide and patchy distribution extending over several kingdoms. An overview of intron distribution, together with the high frequency of eroded heg, suggests that they are evolving towards loss. In this landscape of eroded and lost intron sequences, the A. bisporus cox1 gene exhibits a peculiar dynamics of intron keeping and catching, leading to the largest collection of mitochondrial group I introns reported to date in a Eukaryote. |
format | Text |
id | pubmed-2987802 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-29878022010-12-01 The Agaricus bisporus cox1 Gene: The Longest Mitochondrial Gene and the Largest Reservoir of Mitochondrial Group I Introns Férandon, Cyril Moukha, Serge Callac, Philippe Benedetto, Jean-Pierre Castroviejo, Michel Barroso, Gérard PLoS One Research Article In eukaryotes, introns are located in nuclear and organelle genes from several kingdoms. Large introns (up to 5 kbp) are frequent in mitochondrial genomes of plant and fungi but scarce in Metazoa, even if these organisms are grouped with fungi among the Opisthokonts. Mitochondrial introns are classified in two groups (I and II) according to their RNA secondary structure involved in the intron self-splicing mechanism. Most of these mitochondrial group I introns carry a “Homing Endonuclease Gene” (heg) encoding a DNA endonuclease acting in transfer and site-specific integration (“homing”) and allowing intron spreading and gain after lateral transfer even between species from different kingdoms. Opposed to this gain mechanism, is another which implies that introns, which would have been abundant in the ancestral genes, would mainly evolve by loss. The importance of both mechanisms (loss and gain) is matter of debate. Here we report the sequence of the cox1 gene of the button mushroom Agaricus bisporus, the most widely cultivated mushroom in the world. This gene is both the longest mitochondrial gene (29,902 nt) and the largest group I intron reservoir reported to date with 18 group I and 1 group II. An exhaustive analysis of the group I introns available in cox1 genes shows that they are mobile genetic elements whose numerous events of loss and gain by lateral transfer combine to explain their wide and patchy distribution extending over several kingdoms. An overview of intron distribution, together with the high frequency of eroded heg, suggests that they are evolving towards loss. In this landscape of eroded and lost intron sequences, the A. bisporus cox1 gene exhibits a peculiar dynamics of intron keeping and catching, leading to the largest collection of mitochondrial group I introns reported to date in a Eukaryote. Public Library of Science 2010-11-18 /pmc/articles/PMC2987802/ /pubmed/21124976 http://dx.doi.org/10.1371/journal.pone.0014048 Text en Férandon et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Férandon, Cyril Moukha, Serge Callac, Philippe Benedetto, Jean-Pierre Castroviejo, Michel Barroso, Gérard The Agaricus bisporus cox1 Gene: The Longest Mitochondrial Gene and the Largest Reservoir of Mitochondrial Group I Introns |
title | The Agaricus bisporus cox1 Gene: The Longest Mitochondrial Gene and the Largest Reservoir of Mitochondrial Group I Introns |
title_full | The Agaricus bisporus cox1 Gene: The Longest Mitochondrial Gene and the Largest Reservoir of Mitochondrial Group I Introns |
title_fullStr | The Agaricus bisporus cox1 Gene: The Longest Mitochondrial Gene and the Largest Reservoir of Mitochondrial Group I Introns |
title_full_unstemmed | The Agaricus bisporus cox1 Gene: The Longest Mitochondrial Gene and the Largest Reservoir of Mitochondrial Group I Introns |
title_short | The Agaricus bisporus cox1 Gene: The Longest Mitochondrial Gene and the Largest Reservoir of Mitochondrial Group I Introns |
title_sort | agaricus bisporus cox1 gene: the longest mitochondrial gene and the largest reservoir of mitochondrial group i introns |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2987802/ https://www.ncbi.nlm.nih.gov/pubmed/21124976 http://dx.doi.org/10.1371/journal.pone.0014048 |
work_keys_str_mv | AT ferandoncyril theagaricusbisporuscox1genethelongestmitochondrialgeneandthelargestreservoirofmitochondrialgroupiintrons AT moukhaserge theagaricusbisporuscox1genethelongestmitochondrialgeneandthelargestreservoirofmitochondrialgroupiintrons AT callacphilippe theagaricusbisporuscox1genethelongestmitochondrialgeneandthelargestreservoirofmitochondrialgroupiintrons AT benedettojeanpierre theagaricusbisporuscox1genethelongestmitochondrialgeneandthelargestreservoirofmitochondrialgroupiintrons AT castroviejomichel theagaricusbisporuscox1genethelongestmitochondrialgeneandthelargestreservoirofmitochondrialgroupiintrons AT barrosogerard theagaricusbisporuscox1genethelongestmitochondrialgeneandthelargestreservoirofmitochondrialgroupiintrons AT ferandoncyril agaricusbisporuscox1genethelongestmitochondrialgeneandthelargestreservoirofmitochondrialgroupiintrons AT moukhaserge agaricusbisporuscox1genethelongestmitochondrialgeneandthelargestreservoirofmitochondrialgroupiintrons AT callacphilippe agaricusbisporuscox1genethelongestmitochondrialgeneandthelargestreservoirofmitochondrialgroupiintrons AT benedettojeanpierre agaricusbisporuscox1genethelongestmitochondrialgeneandthelargestreservoirofmitochondrialgroupiintrons AT castroviejomichel agaricusbisporuscox1genethelongestmitochondrialgeneandthelargestreservoirofmitochondrialgroupiintrons AT barrosogerard agaricusbisporuscox1genethelongestmitochondrialgeneandthelargestreservoirofmitochondrialgroupiintrons |