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CO(2) Acts as a Signalling Molecule in Populations of the Fungal Pathogen Candida albicans

When colonising host-niches or non-animated medical devices, individual cells of the fungal pathogen Candida albicans expand into significant biomasses. Here we show that within such biomasses, fungal metabolically generated CO(2) acts as a communication molecule promoting the switch from yeast to f...

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Autores principales: Hall, Rebecca A., De Sordi, Luisa, MacCallum, Donna M., Topal, Hüsnü, Eaton, Rebecca, Bloor, James W., Robinson, Gary K., Levin, Lonny R., Buck, Jochen, Wang, Yue, Gow, Neil A. R., Steegborn, Clemens, Mühlschlegel, Fritz A.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2987819/
https://www.ncbi.nlm.nih.gov/pubmed/21124988
http://dx.doi.org/10.1371/journal.ppat.1001193
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author Hall, Rebecca A.
De Sordi, Luisa
MacCallum, Donna M.
Topal, Hüsnü
Eaton, Rebecca
Bloor, James W.
Robinson, Gary K.
Levin, Lonny R.
Buck, Jochen
Wang, Yue
Gow, Neil A. R.
Steegborn, Clemens
Mühlschlegel, Fritz A.
author_facet Hall, Rebecca A.
De Sordi, Luisa
MacCallum, Donna M.
Topal, Hüsnü
Eaton, Rebecca
Bloor, James W.
Robinson, Gary K.
Levin, Lonny R.
Buck, Jochen
Wang, Yue
Gow, Neil A. R.
Steegborn, Clemens
Mühlschlegel, Fritz A.
author_sort Hall, Rebecca A.
collection PubMed
description When colonising host-niches or non-animated medical devices, individual cells of the fungal pathogen Candida albicans expand into significant biomasses. Here we show that within such biomasses, fungal metabolically generated CO(2) acts as a communication molecule promoting the switch from yeast to filamentous growth essential for C. albicans pathology. We find that CO(2)-mediated intra-colony signalling involves the adenylyl cyclase protein (Cyr1p), a multi-sensor recently found to coordinate fungal responses to serum and bacterial peptidoglycan. We further identify Lys 1373 as essential for CO(2)/bicarbonate regulation of Cyr1p. Disruption of the CO(2)/bicarbonate receptor-site interferes selectively with C. albicans filamentation within fungal biomasses. Comparisons between the Drosophila melanogaster infection model and the mouse model of disseminated candidiasis, suggest that metabolic CO(2) sensing may be important for initial colonisation and epithelial invasion. Our results reveal the existence of a gaseous Candida signalling pathway and its molecular mechanism and provide insights into an evolutionary conserved CO(2)-signalling system.
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spelling pubmed-29878192010-12-01 CO(2) Acts as a Signalling Molecule in Populations of the Fungal Pathogen Candida albicans Hall, Rebecca A. De Sordi, Luisa MacCallum, Donna M. Topal, Hüsnü Eaton, Rebecca Bloor, James W. Robinson, Gary K. Levin, Lonny R. Buck, Jochen Wang, Yue Gow, Neil A. R. Steegborn, Clemens Mühlschlegel, Fritz A. PLoS Pathog Research Article When colonising host-niches or non-animated medical devices, individual cells of the fungal pathogen Candida albicans expand into significant biomasses. Here we show that within such biomasses, fungal metabolically generated CO(2) acts as a communication molecule promoting the switch from yeast to filamentous growth essential for C. albicans pathology. We find that CO(2)-mediated intra-colony signalling involves the adenylyl cyclase protein (Cyr1p), a multi-sensor recently found to coordinate fungal responses to serum and bacterial peptidoglycan. We further identify Lys 1373 as essential for CO(2)/bicarbonate regulation of Cyr1p. Disruption of the CO(2)/bicarbonate receptor-site interferes selectively with C. albicans filamentation within fungal biomasses. Comparisons between the Drosophila melanogaster infection model and the mouse model of disseminated candidiasis, suggest that metabolic CO(2) sensing may be important for initial colonisation and epithelial invasion. Our results reveal the existence of a gaseous Candida signalling pathway and its molecular mechanism and provide insights into an evolutionary conserved CO(2)-signalling system. Public Library of Science 2010-11-18 /pmc/articles/PMC2987819/ /pubmed/21124988 http://dx.doi.org/10.1371/journal.ppat.1001193 Text en Hall et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hall, Rebecca A.
De Sordi, Luisa
MacCallum, Donna M.
Topal, Hüsnü
Eaton, Rebecca
Bloor, James W.
Robinson, Gary K.
Levin, Lonny R.
Buck, Jochen
Wang, Yue
Gow, Neil A. R.
Steegborn, Clemens
Mühlschlegel, Fritz A.
CO(2) Acts as a Signalling Molecule in Populations of the Fungal Pathogen Candida albicans
title CO(2) Acts as a Signalling Molecule in Populations of the Fungal Pathogen Candida albicans
title_full CO(2) Acts as a Signalling Molecule in Populations of the Fungal Pathogen Candida albicans
title_fullStr CO(2) Acts as a Signalling Molecule in Populations of the Fungal Pathogen Candida albicans
title_full_unstemmed CO(2) Acts as a Signalling Molecule in Populations of the Fungal Pathogen Candida albicans
title_short CO(2) Acts as a Signalling Molecule in Populations of the Fungal Pathogen Candida albicans
title_sort co(2) acts as a signalling molecule in populations of the fungal pathogen candida albicans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2987819/
https://www.ncbi.nlm.nih.gov/pubmed/21124988
http://dx.doi.org/10.1371/journal.ppat.1001193
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