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Expressions and clinical significances of CD133 protein and CD133 mRNA in primary lesion of gastric adenocacinoma

BACKGROUND: To study on expressions and clinical significances of CD133 protein and CD133 mRNA in primary lesion of gastric adenocarcinoma (GC). METHODS: Expressions of CD133 protein by immunostaining (99 cases) and CD133 mRNA by semi-quantitative RT-PCR (31 cases) were detected in primary lesion an...

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Autores principales: Yu, Ji-wei, Zhang, Peng, Wu, Ju-gang, Wu, Sheng-hua, Li, Xiao-qiang, Wang, Shi-ting, Lu, Rui-qi, Ni, Xiao-chun, Jiang, Bo-jian
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2987897/
https://www.ncbi.nlm.nih.gov/pubmed/21054902
http://dx.doi.org/10.1186/1756-9966-29-141
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author Yu, Ji-wei
Zhang, Peng
Wu, Ju-gang
Wu, Sheng-hua
Li, Xiao-qiang
Wang, Shi-ting
Lu, Rui-qi
Ni, Xiao-chun
Jiang, Bo-jian
author_facet Yu, Ji-wei
Zhang, Peng
Wu, Ju-gang
Wu, Sheng-hua
Li, Xiao-qiang
Wang, Shi-ting
Lu, Rui-qi
Ni, Xiao-chun
Jiang, Bo-jian
author_sort Yu, Ji-wei
collection PubMed
description BACKGROUND: To study on expressions and clinical significances of CD133 protein and CD133 mRNA in primary lesion of gastric adenocarcinoma (GC). METHODS: Expressions of CD133 protein by immunostaining (99 cases) and CD133 mRNA by semi-quantitative RT-PCR (31 cases) were detected in primary lesion and in noncancerous gastric mucosa tissue (NCGT). Correlations of CD133 protein expression with clinicopathological parameters and post-operative survival were analyzed. Relations of CD133 mRNA level with Ki-67 labeling index (LI), and lymphatic metastasis were assessed too. RESULTS: Brown particles indicating CD133 protein positivity occurred in some parts of tumor cells and epithelium. Expressive percentage of CD133 protein positivity was significantly higher in subgroups with >5 cm diameter (P = 0.041), later TNM stage (P = 0.044), severer lymph node metastasis (P = 0.017), occurrences of lymphatic invasion (P = 0.000) and vascular invasion (P = 0.000) respectively. Severer invasion depth (P = 0.011), lymph node metastasis occurrence (P = 0.043) and later TNM stage (P = 0.049) were the independent risk factors for CD133 protein expression. Average brightness scale value (BSV) of CD133 mRNA was significantly higher in subgroups with >5 cm diameter (P = 0.041), lymph node metastasis occurrence (P = 0.004) and in lower Ki-67 LI (P = 0.02). Relative analysis revealed that BSV of CD133 mRNA related positively to metastatic lymphatic nodes ratio (P = 0.008) and metastatic lymph node number (P = 0.009), but negatively to Ki-67 LI (P = 0.009). Survival of positive subgroup of CD 133 protein was significantly poorer (P = 0.047). Lymph node metastasis occurrence (P = 0.042), later TNM stage (P = 0.046) and CD 133 protein positive expression (P = 0.046) were respectively the independent risk factors to survival. CONCLUSION: Higher expressive level of CD133 mRNA is associated to lower Ki-67 LI and severer lymphatic metastasis. Therefore, the expressive level of CD133 mRNA can play an appropriate role to reflect the status of lymph node metastasis and proliferation of GC. CD133 protein expression is closely related with larger tumor, later TNM stage, lymphtic metastasis and survival of GC.
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spelling pubmed-29878972010-11-19 Expressions and clinical significances of CD133 protein and CD133 mRNA in primary lesion of gastric adenocacinoma Yu, Ji-wei Zhang, Peng Wu, Ju-gang Wu, Sheng-hua Li, Xiao-qiang Wang, Shi-ting Lu, Rui-qi Ni, Xiao-chun Jiang, Bo-jian J Exp Clin Cancer Res Research BACKGROUND: To study on expressions and clinical significances of CD133 protein and CD133 mRNA in primary lesion of gastric adenocarcinoma (GC). METHODS: Expressions of CD133 protein by immunostaining (99 cases) and CD133 mRNA by semi-quantitative RT-PCR (31 cases) were detected in primary lesion and in noncancerous gastric mucosa tissue (NCGT). Correlations of CD133 protein expression with clinicopathological parameters and post-operative survival were analyzed. Relations of CD133 mRNA level with Ki-67 labeling index (LI), and lymphatic metastasis were assessed too. RESULTS: Brown particles indicating CD133 protein positivity occurred in some parts of tumor cells and epithelium. Expressive percentage of CD133 protein positivity was significantly higher in subgroups with >5 cm diameter (P = 0.041), later TNM stage (P = 0.044), severer lymph node metastasis (P = 0.017), occurrences of lymphatic invasion (P = 0.000) and vascular invasion (P = 0.000) respectively. Severer invasion depth (P = 0.011), lymph node metastasis occurrence (P = 0.043) and later TNM stage (P = 0.049) were the independent risk factors for CD133 protein expression. Average brightness scale value (BSV) of CD133 mRNA was significantly higher in subgroups with >5 cm diameter (P = 0.041), lymph node metastasis occurrence (P = 0.004) and in lower Ki-67 LI (P = 0.02). Relative analysis revealed that BSV of CD133 mRNA related positively to metastatic lymphatic nodes ratio (P = 0.008) and metastatic lymph node number (P = 0.009), but negatively to Ki-67 LI (P = 0.009). Survival of positive subgroup of CD 133 protein was significantly poorer (P = 0.047). Lymph node metastasis occurrence (P = 0.042), later TNM stage (P = 0.046) and CD 133 protein positive expression (P = 0.046) were respectively the independent risk factors to survival. CONCLUSION: Higher expressive level of CD133 mRNA is associated to lower Ki-67 LI and severer lymphatic metastasis. Therefore, the expressive level of CD133 mRNA can play an appropriate role to reflect the status of lymph node metastasis and proliferation of GC. CD133 protein expression is closely related with larger tumor, later TNM stage, lymphtic metastasis and survival of GC. BioMed Central 2010-11-07 /pmc/articles/PMC2987897/ /pubmed/21054902 http://dx.doi.org/10.1186/1756-9966-29-141 Text en Copyright ©2010 Yu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Yu, Ji-wei
Zhang, Peng
Wu, Ju-gang
Wu, Sheng-hua
Li, Xiao-qiang
Wang, Shi-ting
Lu, Rui-qi
Ni, Xiao-chun
Jiang, Bo-jian
Expressions and clinical significances of CD133 protein and CD133 mRNA in primary lesion of gastric adenocacinoma
title Expressions and clinical significances of CD133 protein and CD133 mRNA in primary lesion of gastric adenocacinoma
title_full Expressions and clinical significances of CD133 protein and CD133 mRNA in primary lesion of gastric adenocacinoma
title_fullStr Expressions and clinical significances of CD133 protein and CD133 mRNA in primary lesion of gastric adenocacinoma
title_full_unstemmed Expressions and clinical significances of CD133 protein and CD133 mRNA in primary lesion of gastric adenocacinoma
title_short Expressions and clinical significances of CD133 protein and CD133 mRNA in primary lesion of gastric adenocacinoma
title_sort expressions and clinical significances of cd133 protein and cd133 mrna in primary lesion of gastric adenocacinoma
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2987897/
https://www.ncbi.nlm.nih.gov/pubmed/21054902
http://dx.doi.org/10.1186/1756-9966-29-141
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