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Breakpoint structure of the Anopheles gambiae 2Rb chromosomal inversion
BACKGROUND: Alternative arrangements of chromosome 2 inversions in Anopheles gambiae are important sources of population structure, and are associated with adaptation to environmental heterogeneity. The forces responsible for their origin and maintenance are incompletely understood. Molecular charac...
Autores principales: | , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2988034/ https://www.ncbi.nlm.nih.gov/pubmed/20974007 http://dx.doi.org/10.1186/1475-2875-9-293 |
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author | Lobo, Neil F Sangaré, Djibril M Regier, Allison A Reidenbach, Kyanne R Bretz, David A Sharakhova, Maria V Emrich, Scott J Traore, Sekou F Costantini, Carlo Besansky, Nora J Collins, Frank H |
author_facet | Lobo, Neil F Sangaré, Djibril M Regier, Allison A Reidenbach, Kyanne R Bretz, David A Sharakhova, Maria V Emrich, Scott J Traore, Sekou F Costantini, Carlo Besansky, Nora J Collins, Frank H |
author_sort | Lobo, Neil F |
collection | PubMed |
description | BACKGROUND: Alternative arrangements of chromosome 2 inversions in Anopheles gambiae are important sources of population structure, and are associated with adaptation to environmental heterogeneity. The forces responsible for their origin and maintenance are incompletely understood. Molecular characterization of inversion breakpoints provides insight into how they arose, and provides the basis for development of molecular karyotyping methods useful in future studies. METHODS: Sequence comparison of regions near the cytological breakpoints of 2Rb allowed the molecular delineation of breakpoint boundaries. Comparisons were made between the standard 2R+(b )arrangement in the An. gambiae PEST reference genome and the inverted 2Rb arrangements in the An. gambiae M and S genome assemblies. Sequence differences between alternative 2Rb arrangements were exploited in the design of a PCR diagnostic assay, which was evaluated against the known chromosomal banding pattern of laboratory colonies and field-collected samples from Mali and Cameroon. RESULTS: The breakpoints of the 7.55 Mb 2Rb inversion are flanked by extensive runs of the same short (72 bp) tandemly organized sequence, which was likely responsible for chromosomal breakage and rearrangement. Application of the molecular diagnostic assay suggested that 2Rb has a single common origin in An. gambiae and its sibling species, Anopheles arabiensis, and also that the standard arrangement (2R+(b)) may have arisen twice through breakpoint reuse. The molecular diagnostic was reliable when applied to laboratory colonies, but its accuracy was lower in natural populations. CONCLUSIONS: The complex repetitive sequence flanking the 2Rb breakpoint region may be prone to structural and sequence-level instability. The 2Rb molecular diagnostic has immediate application in studies based on laboratory colonies, but its usefulness in natural populations awaits development of complementary molecular tools. |
format | Text |
id | pubmed-2988034 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-29880342010-11-19 Breakpoint structure of the Anopheles gambiae 2Rb chromosomal inversion Lobo, Neil F Sangaré, Djibril M Regier, Allison A Reidenbach, Kyanne R Bretz, David A Sharakhova, Maria V Emrich, Scott J Traore, Sekou F Costantini, Carlo Besansky, Nora J Collins, Frank H Malar J Research BACKGROUND: Alternative arrangements of chromosome 2 inversions in Anopheles gambiae are important sources of population structure, and are associated with adaptation to environmental heterogeneity. The forces responsible for their origin and maintenance are incompletely understood. Molecular characterization of inversion breakpoints provides insight into how they arose, and provides the basis for development of molecular karyotyping methods useful in future studies. METHODS: Sequence comparison of regions near the cytological breakpoints of 2Rb allowed the molecular delineation of breakpoint boundaries. Comparisons were made between the standard 2R+(b )arrangement in the An. gambiae PEST reference genome and the inverted 2Rb arrangements in the An. gambiae M and S genome assemblies. Sequence differences between alternative 2Rb arrangements were exploited in the design of a PCR diagnostic assay, which was evaluated against the known chromosomal banding pattern of laboratory colonies and field-collected samples from Mali and Cameroon. RESULTS: The breakpoints of the 7.55 Mb 2Rb inversion are flanked by extensive runs of the same short (72 bp) tandemly organized sequence, which was likely responsible for chromosomal breakage and rearrangement. Application of the molecular diagnostic assay suggested that 2Rb has a single common origin in An. gambiae and its sibling species, Anopheles arabiensis, and also that the standard arrangement (2R+(b)) may have arisen twice through breakpoint reuse. The molecular diagnostic was reliable when applied to laboratory colonies, but its accuracy was lower in natural populations. CONCLUSIONS: The complex repetitive sequence flanking the 2Rb breakpoint region may be prone to structural and sequence-level instability. The 2Rb molecular diagnostic has immediate application in studies based on laboratory colonies, but its usefulness in natural populations awaits development of complementary molecular tools. BioMed Central 2010-10-25 /pmc/articles/PMC2988034/ /pubmed/20974007 http://dx.doi.org/10.1186/1475-2875-9-293 Text en Copyright ©2010 Lobo et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Lobo, Neil F Sangaré, Djibril M Regier, Allison A Reidenbach, Kyanne R Bretz, David A Sharakhova, Maria V Emrich, Scott J Traore, Sekou F Costantini, Carlo Besansky, Nora J Collins, Frank H Breakpoint structure of the Anopheles gambiae 2Rb chromosomal inversion |
title | Breakpoint structure of the Anopheles gambiae 2Rb chromosomal inversion |
title_full | Breakpoint structure of the Anopheles gambiae 2Rb chromosomal inversion |
title_fullStr | Breakpoint structure of the Anopheles gambiae 2Rb chromosomal inversion |
title_full_unstemmed | Breakpoint structure of the Anopheles gambiae 2Rb chromosomal inversion |
title_short | Breakpoint structure of the Anopheles gambiae 2Rb chromosomal inversion |
title_sort | breakpoint structure of the anopheles gambiae 2rb chromosomal inversion |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2988034/ https://www.ncbi.nlm.nih.gov/pubmed/20974007 http://dx.doi.org/10.1186/1475-2875-9-293 |
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