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Serum microRNA characterization identifies miR-885-5p as a potential marker for detecting liver pathologies

Circulating miRNAs (microRNAs) are emerging as promising biomarkers for several pathological conditions, and the aim of this study was to investigate the feasibility of using serum miRNAs as biomarkers for liver pathologies. Real-time qPCR (quantitative PCR)-based TaqMan MicroRNA arrays were first e...

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Autores principales: Gui, Junhao, Tian, Yaping, Wen, Xinyu, Zhang, Wenhui, Zhang, Pengjun, Gao, Jing, Run, Wei, Tian, Liyuan, Jia, Xingwang, Gao, Yanhong
Formato: Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2990200/
https://www.ncbi.nlm.nih.gov/pubmed/20815808
http://dx.doi.org/10.1042/CS20100297
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author Gui, Junhao
Tian, Yaping
Wen, Xinyu
Zhang, Wenhui
Zhang, Pengjun
Gao, Jing
Run, Wei
Tian, Liyuan
Jia, Xingwang
Gao, Yanhong
author_facet Gui, Junhao
Tian, Yaping
Wen, Xinyu
Zhang, Wenhui
Zhang, Pengjun
Gao, Jing
Run, Wei
Tian, Liyuan
Jia, Xingwang
Gao, Yanhong
author_sort Gui, Junhao
collection PubMed
description Circulating miRNAs (microRNAs) are emerging as promising biomarkers for several pathological conditions, and the aim of this study was to investigate the feasibility of using serum miRNAs as biomarkers for liver pathologies. Real-time qPCR (quantitative PCR)-based TaqMan MicroRNA arrays were first employed to profile miRNAs in serum pools from patients with HCC (hepatocellular carcinoma) or LC (liver cirrhosis) and from healthy controls. Five miRNAs (i.e. miR-885-5p, miR-574-3p, miR-224, miR-215 and miR-146a) that were up-regulated in the HCC and LC serum pools were selected and further quantified using real-time qPCR in patients with HCC, LC, CHB (chronic hepatitis B) or GC (gastric cancer) and in normal controls. The present study revealed that more than 110 miRNA species in the serum samples and wide distribution ranges of serum miRNAs were observed. The levels of miR-885-5p were significantly higher in sera from patients with HCC, LC and CHB than in healthy controls or GC patients. miR-885-5p yielded an AUC [the area under the ROC (receiver operating characteristic) curve] of 0.904 [95% CI (confidence interval), 0.837–0.951, P<0.0001) with 90.53% sensitivity and 79.17% specificity in discriminating liver pathologies from healthy controls, using a cut off value of 1.06 (normalized). No correlations between increased miR-885-5p and liver function parameters [AFP (α-fetoprotein), ALT (alanine aminotransferase), AST (aspartate aminotransferase) and GGT (γ-glutamyl transpeptidase)] were observed in patients with liver pathologies. In summary, miR-885-5p is significantly elevated in the sera of patients with liver pathologies, and our data suggest that serum miRNAs could serve as novel complementary biomarkers for the detection and assessment of liver pathologies.
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spelling pubmed-29902002010-11-24 Serum microRNA characterization identifies miR-885-5p as a potential marker for detecting liver pathologies Gui, Junhao Tian, Yaping Wen, Xinyu Zhang, Wenhui Zhang, Pengjun Gao, Jing Run, Wei Tian, Liyuan Jia, Xingwang Gao, Yanhong Clin Sci (Lond) Research Article Circulating miRNAs (microRNAs) are emerging as promising biomarkers for several pathological conditions, and the aim of this study was to investigate the feasibility of using serum miRNAs as biomarkers for liver pathologies. Real-time qPCR (quantitative PCR)-based TaqMan MicroRNA arrays were first employed to profile miRNAs in serum pools from patients with HCC (hepatocellular carcinoma) or LC (liver cirrhosis) and from healthy controls. Five miRNAs (i.e. miR-885-5p, miR-574-3p, miR-224, miR-215 and miR-146a) that were up-regulated in the HCC and LC serum pools were selected and further quantified using real-time qPCR in patients with HCC, LC, CHB (chronic hepatitis B) or GC (gastric cancer) and in normal controls. The present study revealed that more than 110 miRNA species in the serum samples and wide distribution ranges of serum miRNAs were observed. The levels of miR-885-5p were significantly higher in sera from patients with HCC, LC and CHB than in healthy controls or GC patients. miR-885-5p yielded an AUC [the area under the ROC (receiver operating characteristic) curve] of 0.904 [95% CI (confidence interval), 0.837–0.951, P<0.0001) with 90.53% sensitivity and 79.17% specificity in discriminating liver pathologies from healthy controls, using a cut off value of 1.06 (normalized). No correlations between increased miR-885-5p and liver function parameters [AFP (α-fetoprotein), ALT (alanine aminotransferase), AST (aspartate aminotransferase) and GGT (γ-glutamyl transpeptidase)] were observed in patients with liver pathologies. In summary, miR-885-5p is significantly elevated in the sera of patients with liver pathologies, and our data suggest that serum miRNAs could serve as novel complementary biomarkers for the detection and assessment of liver pathologies. Portland Press Ltd. 2010-11-19 2011-03-01 /pmc/articles/PMC2990200/ /pubmed/20815808 http://dx.doi.org/10.1042/CS20100297 Text en © 2011 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by-nc/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gui, Junhao
Tian, Yaping
Wen, Xinyu
Zhang, Wenhui
Zhang, Pengjun
Gao, Jing
Run, Wei
Tian, Liyuan
Jia, Xingwang
Gao, Yanhong
Serum microRNA characterization identifies miR-885-5p as a potential marker for detecting liver pathologies
title Serum microRNA characterization identifies miR-885-5p as a potential marker for detecting liver pathologies
title_full Serum microRNA characterization identifies miR-885-5p as a potential marker for detecting liver pathologies
title_fullStr Serum microRNA characterization identifies miR-885-5p as a potential marker for detecting liver pathologies
title_full_unstemmed Serum microRNA characterization identifies miR-885-5p as a potential marker for detecting liver pathologies
title_short Serum microRNA characterization identifies miR-885-5p as a potential marker for detecting liver pathologies
title_sort serum microrna characterization identifies mir-885-5p as a potential marker for detecting liver pathologies
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2990200/
https://www.ncbi.nlm.nih.gov/pubmed/20815808
http://dx.doi.org/10.1042/CS20100297
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