Preparation of Rat Gingival Mitochondria with an Improved Isolation Method

In order to establish a method of obtaining rat gingival mitochondria (Mt), Mt fractions were prepared in various combinations of homogenizing time with collagenase concentration. Rat gingival tissues were excised, minced, treated with collagenase, homogenized, and subjected to differential centrifu...

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Autores principales: Kaneko, Noriaki, Rikimaru, Tetsuya, Fujimura, Tetsuyuki, Mori, Shigeyasu, Hidaka, Saburo, Kaya, Hidehiro
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2990858/
https://www.ncbi.nlm.nih.gov/pubmed/21113439
http://dx.doi.org/10.1155/2010/275103
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author Kaneko, Noriaki
Rikimaru, Tetsuya
Fujimura, Tetsuyuki
Mori, Shigeyasu
Hidaka, Saburo
Kaya, Hidehiro
author_facet Kaneko, Noriaki
Rikimaru, Tetsuya
Fujimura, Tetsuyuki
Mori, Shigeyasu
Hidaka, Saburo
Kaya, Hidehiro
author_sort Kaneko, Noriaki
collection PubMed
description In order to establish a method of obtaining rat gingival mitochondria (Mt), Mt fractions were prepared in various combinations of homogenizing time with collagenase concentration. Rat gingival tissues were excised, minced, treated with collagenase, homogenized, and subjected to differential centrifugation rates. Both the respiratory control ratio (RCR) and adenosine diphosphate/oxygen (ADP/O) ratio of the Mt fraction prepared in a combination of 40, 50, or 60 sec homogenization with collagenase in a concentration range of 0.115%–0.130% (w/v) were measured. The values for the RCR and ADP/O ratio of the Mt fraction obtained in an optimal condition was 1.80 ± 0.05 and 1.65 ± 0.03, respectively. These results suggest that Mt of fairly high quality can be obtained through this refined combination of the homogenizing time and collagenase concentration.
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spelling pubmed-29908582010-11-26 Preparation of Rat Gingival Mitochondria with an Improved Isolation Method Kaneko, Noriaki Rikimaru, Tetsuya Fujimura, Tetsuyuki Mori, Shigeyasu Hidaka, Saburo Kaya, Hidehiro Int J Dent Research Article In order to establish a method of obtaining rat gingival mitochondria (Mt), Mt fractions were prepared in various combinations of homogenizing time with collagenase concentration. Rat gingival tissues were excised, minced, treated with collagenase, homogenized, and subjected to differential centrifugation rates. Both the respiratory control ratio (RCR) and adenosine diphosphate/oxygen (ADP/O) ratio of the Mt fraction prepared in a combination of 40, 50, or 60 sec homogenization with collagenase in a concentration range of 0.115%–0.130% (w/v) were measured. The values for the RCR and ADP/O ratio of the Mt fraction obtained in an optimal condition was 1.80 ± 0.05 and 1.65 ± 0.03, respectively. These results suggest that Mt of fairly high quality can be obtained through this refined combination of the homogenizing time and collagenase concentration. Hindawi Publishing Corporation 2010 2010-11-21 /pmc/articles/PMC2990858/ /pubmed/21113439 http://dx.doi.org/10.1155/2010/275103 Text en Copyright © 2010 Noriaki Kaneko et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kaneko, Noriaki
Rikimaru, Tetsuya
Fujimura, Tetsuyuki
Mori, Shigeyasu
Hidaka, Saburo
Kaya, Hidehiro
Preparation of Rat Gingival Mitochondria with an Improved Isolation Method
title Preparation of Rat Gingival Mitochondria with an Improved Isolation Method
title_full Preparation of Rat Gingival Mitochondria with an Improved Isolation Method
title_fullStr Preparation of Rat Gingival Mitochondria with an Improved Isolation Method
title_full_unstemmed Preparation of Rat Gingival Mitochondria with an Improved Isolation Method
title_short Preparation of Rat Gingival Mitochondria with an Improved Isolation Method
title_sort preparation of rat gingival mitochondria with an improved isolation method
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2990858/
https://www.ncbi.nlm.nih.gov/pubmed/21113439
http://dx.doi.org/10.1155/2010/275103
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