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Identification of a cytokine network sustaining neutrophil and Th17 activation in untreated early rheumatoid arthritis
INTRODUCTION: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease characterized by sustained synovitis. Recently, several studies have proposed neutrophils and Th17 cells as key players in the onset and perpetuation of this disease. The main goal of this work was to determine whet...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2991033/ https://www.ncbi.nlm.nih.gov/pubmed/20961415 http://dx.doi.org/10.1186/ar3168 |
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author | Cascão, Rita Moura, Rita A Perpétuo, Inês Canhão, Helena Vieira-Sousa, Elsa Mourão, Ana F Rodrigues, Ana M Polido-Pereira, Joaquim Queiroz, Mário V Rosário, Henrique S Souto-Carneiro, Maria M Graca, Luis Fonseca, João E |
author_facet | Cascão, Rita Moura, Rita A Perpétuo, Inês Canhão, Helena Vieira-Sousa, Elsa Mourão, Ana F Rodrigues, Ana M Polido-Pereira, Joaquim Queiroz, Mário V Rosário, Henrique S Souto-Carneiro, Maria M Graca, Luis Fonseca, João E |
author_sort | Cascão, Rita |
collection | PubMed |
description | INTRODUCTION: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease characterized by sustained synovitis. Recently, several studies have proposed neutrophils and Th17 cells as key players in the onset and perpetuation of this disease. The main goal of this work was to determine whether cytokines driving neutrophil and Th17 activation are dysregulated in very early rheumatoid arthritis patients with less than 6 weeks of disease duration and before treatment (VERA). METHODS: Cytokines related to neutrophil and Th17 activation were quantified in the serum of VERA and established RA patients and compared with other very early arthritis (VEA) and healthy controls. Synovial fluid (SF) from RA and osteoarthritis (OA) patients was also analyzed. RESULTS: VERA patients had increased serum levels of cytokines promoting Th17 polarization (IL-1β and IL-6), as well as IL-8 and Th17-derived cytokines (IL-17A and IL-22) known to induce neutrophil-mediated inflammation. In established RA this pattern is more evident within the SF. Early treatment with methotrexate or corticosteroids led to clinical improvement but without an impact on the cytokine pattern. CONCLUSIONS: VERA patients already display increased levels of cytokines related with Th17 polarization and neutrophil recruitment and activation, a dysregulation also found in SF of established RA. 0 Thus, our data suggest that a cytokine-milieu favoring Th17 and neutrophil activity is an early event in RA pathogenesis. |
format | Text |
id | pubmed-2991033 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-29910332010-11-25 Identification of a cytokine network sustaining neutrophil and Th17 activation in untreated early rheumatoid arthritis Cascão, Rita Moura, Rita A Perpétuo, Inês Canhão, Helena Vieira-Sousa, Elsa Mourão, Ana F Rodrigues, Ana M Polido-Pereira, Joaquim Queiroz, Mário V Rosário, Henrique S Souto-Carneiro, Maria M Graca, Luis Fonseca, João E Arthritis Res Ther Research Article INTRODUCTION: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease characterized by sustained synovitis. Recently, several studies have proposed neutrophils and Th17 cells as key players in the onset and perpetuation of this disease. The main goal of this work was to determine whether cytokines driving neutrophil and Th17 activation are dysregulated in very early rheumatoid arthritis patients with less than 6 weeks of disease duration and before treatment (VERA). METHODS: Cytokines related to neutrophil and Th17 activation were quantified in the serum of VERA and established RA patients and compared with other very early arthritis (VEA) and healthy controls. Synovial fluid (SF) from RA and osteoarthritis (OA) patients was also analyzed. RESULTS: VERA patients had increased serum levels of cytokines promoting Th17 polarization (IL-1β and IL-6), as well as IL-8 and Th17-derived cytokines (IL-17A and IL-22) known to induce neutrophil-mediated inflammation. In established RA this pattern is more evident within the SF. Early treatment with methotrexate or corticosteroids led to clinical improvement but without an impact on the cytokine pattern. CONCLUSIONS: VERA patients already display increased levels of cytokines related with Th17 polarization and neutrophil recruitment and activation, a dysregulation also found in SF of established RA. 0 Thus, our data suggest that a cytokine-milieu favoring Th17 and neutrophil activity is an early event in RA pathogenesis. BioMed Central 2010 2010-10-20 /pmc/articles/PMC2991033/ /pubmed/20961415 http://dx.doi.org/10.1186/ar3168 Text en Copyright ©2010 Cascão et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cascão, Rita Moura, Rita A Perpétuo, Inês Canhão, Helena Vieira-Sousa, Elsa Mourão, Ana F Rodrigues, Ana M Polido-Pereira, Joaquim Queiroz, Mário V Rosário, Henrique S Souto-Carneiro, Maria M Graca, Luis Fonseca, João E Identification of a cytokine network sustaining neutrophil and Th17 activation in untreated early rheumatoid arthritis |
title | Identification of a cytokine network sustaining neutrophil and Th17 activation in untreated early rheumatoid arthritis |
title_full | Identification of a cytokine network sustaining neutrophil and Th17 activation in untreated early rheumatoid arthritis |
title_fullStr | Identification of a cytokine network sustaining neutrophil and Th17 activation in untreated early rheumatoid arthritis |
title_full_unstemmed | Identification of a cytokine network sustaining neutrophil and Th17 activation in untreated early rheumatoid arthritis |
title_short | Identification of a cytokine network sustaining neutrophil and Th17 activation in untreated early rheumatoid arthritis |
title_sort | identification of a cytokine network sustaining neutrophil and th17 activation in untreated early rheumatoid arthritis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2991033/ https://www.ncbi.nlm.nih.gov/pubmed/20961415 http://dx.doi.org/10.1186/ar3168 |
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