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Collagen-induced arthritis in common marmosets: a new nonhuman primate model for chronic arthritis

INTRODUCTION: There is an ever-increasing need for animal models to evaluate efficacy and safety of new therapeutics in the field of rheumatoid arthritis (RA). Particularly for the early preclinical evaluation of human-specific biologicals targeting the progressive phase of the disease, there is a n...

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Autores principales: Vierboom, Michel PM, Breedveld, Elia, Kondova, Ivanela, 't Hart, Bert A
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2991037/
https://www.ncbi.nlm.nih.gov/pubmed/20977720
http://dx.doi.org/10.1186/ar3172
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author Vierboom, Michel PM
Breedveld, Elia
Kondova, Ivanela
't Hart, Bert A
author_facet Vierboom, Michel PM
Breedveld, Elia
Kondova, Ivanela
't Hart, Bert A
author_sort Vierboom, Michel PM
collection PubMed
description INTRODUCTION: There is an ever-increasing need for animal models to evaluate efficacy and safety of new therapeutics in the field of rheumatoid arthritis (RA). Particularly for the early preclinical evaluation of human-specific biologicals targeting the progressive phase of the disease, there is a need for relevant animal models. In response to this requirement we set out to develop a model of collagen-induced arthritis (CIA) in a small-sized nonhuman primate species (300 to 400 g at adult age); that is, the common marmoset (Callithrix jacchus). METHODS: Twenty-two animals divided into three experiments were immunized with collagen type II (CII) of either bovine or chicken origin with different immunization strategies. The animals were analyzed for clinical manifestation of arthritis, hematology and clinical chemistry, immunological responses against CII and histopathological features of the arthritis. RESULTS: Clinically manifest arthritis was observed in almost 100% (21 out of 22) of the animals. Fifty percent of the animals developed semi-acute CIA while the other 50% displayed a more chronic disease. Both cellular (CD3/CD4 and CD3/CD8) and humoral responses (IgM and IgG) against CII were involved in the development of the disease. Besides mild histopathological changes in bone and cartilage, severe inflammation in extraarticular tissues like periosteum and subcutaneous tissues was observed. CONCLUSIONS: This new model in marmosets more closely resembles chronic RA with respect to the chronic disease course and pathomorphological presentation than the more acute monophasic and destructive CIA model in macaques. This model can therefore fill a niche in preclinical testing of new human specific therapeutics.
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spelling pubmed-29910372010-12-13 Collagen-induced arthritis in common marmosets: a new nonhuman primate model for chronic arthritis Vierboom, Michel PM Breedveld, Elia Kondova, Ivanela 't Hart, Bert A Arthritis Res Ther Research Article INTRODUCTION: There is an ever-increasing need for animal models to evaluate efficacy and safety of new therapeutics in the field of rheumatoid arthritis (RA). Particularly for the early preclinical evaluation of human-specific biologicals targeting the progressive phase of the disease, there is a need for relevant animal models. In response to this requirement we set out to develop a model of collagen-induced arthritis (CIA) in a small-sized nonhuman primate species (300 to 400 g at adult age); that is, the common marmoset (Callithrix jacchus). METHODS: Twenty-two animals divided into three experiments were immunized with collagen type II (CII) of either bovine or chicken origin with different immunization strategies. The animals were analyzed for clinical manifestation of arthritis, hematology and clinical chemistry, immunological responses against CII and histopathological features of the arthritis. RESULTS: Clinically manifest arthritis was observed in almost 100% (21 out of 22) of the animals. Fifty percent of the animals developed semi-acute CIA while the other 50% displayed a more chronic disease. Both cellular (CD3/CD4 and CD3/CD8) and humoral responses (IgM and IgG) against CII were involved in the development of the disease. Besides mild histopathological changes in bone and cartilage, severe inflammation in extraarticular tissues like periosteum and subcutaneous tissues was observed. CONCLUSIONS: This new model in marmosets more closely resembles chronic RA with respect to the chronic disease course and pathomorphological presentation than the more acute monophasic and destructive CIA model in macaques. This model can therefore fill a niche in preclinical testing of new human specific therapeutics. BioMed Central 2010 2010-10-26 /pmc/articles/PMC2991037/ /pubmed/20977720 http://dx.doi.org/10.1186/ar3172 Text en Copyright ©2010 Vierboom et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Vierboom, Michel PM
Breedveld, Elia
Kondova, Ivanela
't Hart, Bert A
Collagen-induced arthritis in common marmosets: a new nonhuman primate model for chronic arthritis
title Collagen-induced arthritis in common marmosets: a new nonhuman primate model for chronic arthritis
title_full Collagen-induced arthritis in common marmosets: a new nonhuman primate model for chronic arthritis
title_fullStr Collagen-induced arthritis in common marmosets: a new nonhuman primate model for chronic arthritis
title_full_unstemmed Collagen-induced arthritis in common marmosets: a new nonhuman primate model for chronic arthritis
title_short Collagen-induced arthritis in common marmosets: a new nonhuman primate model for chronic arthritis
title_sort collagen-induced arthritis in common marmosets: a new nonhuman primate model for chronic arthritis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2991037/
https://www.ncbi.nlm.nih.gov/pubmed/20977720
http://dx.doi.org/10.1186/ar3172
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