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Crenarchaeal Biofilm Formation under Extreme Conditions

BACKGROUND: Biofilm formation has been studied in much detail for a variety of bacterial species, as it plays a major role in the pathogenicity of bacteria. However, only limited information is available for the development of archaeal communities that are frequently found in many natural environmen...

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Autores principales: Koerdt, Andrea, Gödeke, Julia, Berger, Jürgen, Thormann, Kai M., Albers, Sonja-Verena
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2991349/
https://www.ncbi.nlm.nih.gov/pubmed/21124788
http://dx.doi.org/10.1371/journal.pone.0014104
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author Koerdt, Andrea
Gödeke, Julia
Berger, Jürgen
Thormann, Kai M.
Albers, Sonja-Verena
author_facet Koerdt, Andrea
Gödeke, Julia
Berger, Jürgen
Thormann, Kai M.
Albers, Sonja-Verena
author_sort Koerdt, Andrea
collection PubMed
description BACKGROUND: Biofilm formation has been studied in much detail for a variety of bacterial species, as it plays a major role in the pathogenicity of bacteria. However, only limited information is available for the development of archaeal communities that are frequently found in many natural environments. METHODOLOGY: We have analyzed biofilm formation in three closely related hyperthermophilic crenarchaeotes: Sulfolobus acidocaldarius, S. solfataricus and S. tokodaii. We established a microtitre plate assay adapted to high temperatures to determine how pH and temperature influence biofilm formation in these organisms. Biofilm analysis by confocal laser scanning microscopy demonstrated that the three strains form very different communities ranging from simple carpet-like structures in S. solfataricus to high density tower-like structures in S. acidocaldarius in static systems. Lectin staining indicated that all three strains produced extracellular polysaccharides containing glucose, galactose, mannose and N-acetylglucosamine once biofilm formation was initiated. While flagella mutants had no phenotype in two days old static biofilms of S. solfataricus, a UV-induced pili deletion mutant showed decreased attachment of cells. CONCLUSION: The study gives first insights into formation and development of crenarchaeal biofilms in extreme environments.
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spelling pubmed-29913492010-12-01 Crenarchaeal Biofilm Formation under Extreme Conditions Koerdt, Andrea Gödeke, Julia Berger, Jürgen Thormann, Kai M. Albers, Sonja-Verena PLoS One Research Article BACKGROUND: Biofilm formation has been studied in much detail for a variety of bacterial species, as it plays a major role in the pathogenicity of bacteria. However, only limited information is available for the development of archaeal communities that are frequently found in many natural environments. METHODOLOGY: We have analyzed biofilm formation in three closely related hyperthermophilic crenarchaeotes: Sulfolobus acidocaldarius, S. solfataricus and S. tokodaii. We established a microtitre plate assay adapted to high temperatures to determine how pH and temperature influence biofilm formation in these organisms. Biofilm analysis by confocal laser scanning microscopy demonstrated that the three strains form very different communities ranging from simple carpet-like structures in S. solfataricus to high density tower-like structures in S. acidocaldarius in static systems. Lectin staining indicated that all three strains produced extracellular polysaccharides containing glucose, galactose, mannose and N-acetylglucosamine once biofilm formation was initiated. While flagella mutants had no phenotype in two days old static biofilms of S. solfataricus, a UV-induced pili deletion mutant showed decreased attachment of cells. CONCLUSION: The study gives first insights into formation and development of crenarchaeal biofilms in extreme environments. Public Library of Science 2010-11-24 /pmc/articles/PMC2991349/ /pubmed/21124788 http://dx.doi.org/10.1371/journal.pone.0014104 Text en Koerdt et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Koerdt, Andrea
Gödeke, Julia
Berger, Jürgen
Thormann, Kai M.
Albers, Sonja-Verena
Crenarchaeal Biofilm Formation under Extreme Conditions
title Crenarchaeal Biofilm Formation under Extreme Conditions
title_full Crenarchaeal Biofilm Formation under Extreme Conditions
title_fullStr Crenarchaeal Biofilm Formation under Extreme Conditions
title_full_unstemmed Crenarchaeal Biofilm Formation under Extreme Conditions
title_short Crenarchaeal Biofilm Formation under Extreme Conditions
title_sort crenarchaeal biofilm formation under extreme conditions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2991349/
https://www.ncbi.nlm.nih.gov/pubmed/21124788
http://dx.doi.org/10.1371/journal.pone.0014104
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