Cargando…

High-Yield Expression of Heterologous [FeFe] Hydrogenases in Escherichia coli

BACKGROUND: The realization of hydrogenase-based technologies for renewable H(2) production is presently limited by the need for scalable and high-yielding methods to supply active hydrogenases and their required maturases. PRINCIPAL FINDINGS: In this report, we describe an improved Escherichia coli...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kuchenreuther, Jon M., Grady-Smith, Celestine S., Bingham, Alyssa S., George, Simon J., Cramer, Stephen P., Swartz, James R.
Formato:	Texto
Lenguaje:	English
Publicado:	Public Library of Science 2010
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2991362/ https://www.ncbi.nlm.nih.gov/pubmed/21124800 http://dx.doi.org/10.1371/journal.pone.0015491

Ejemplares similares

Cell-free H-cluster Synthesis and [FeFe] Hydrogenase Activation: All Five CO and CN(−) Ligands Derive from Tyrosine
por: Kuchenreuther, Jon M., et al.
Publicado: (2011)

New Insights into [FeFe] Hydrogenase Activation and Maturase Function
por: Kuchenreuther, Jon M., et al.
Publicado: (2012)

Tyrosine, Cysteine, and S-Adenosyl Methionine Stimulate In Vitro [FeFe] Hydrogenase Activation
por: Kuchenreuther, Jon M., et al.
Publicado: (2009)

Integration of an [FeFe]-hydrogenase into the anaerobic metabolism of Escherichia coli
por: Kelly, Ciarán L., et al.
Publicado: (2015)

The Cyanide Ligands of [FeFe] Hydrogenase: Pulse EPR Studies of (13)C and (15)N-Labeled H-Cluster
por: Myers, William K., et al.
Publicado: (2014)

Energetics for Proton Reduction in FeFe Hydrogenase
por: Siegbahn, Per E. M., et al.
Publicado: (2020)

Inhibition of [FeFe]-hydrogenase by formaldehyde: proposed mechanism and reactivity of FeFe alkyl complexes
por: Zhang, Fanjun, et al.
Publicado: (2021)

Fantastic [FeFe]-Hydrogenases and Where to Find Them
por: Morra, Simone
Publicado: (2022)

[NiFe], [FeFe], and [Fe] hydrogenase models from isomers
por: Ogo, Seiji, et al.
Publicado: (2020)

The Beta Subunit of Non-bifurcating NADH-Dependent [FeFe]-Hydrogenases Differs From Those of Multimeric Electron-Bifurcating [FeFe]-Hydrogenases
por: Losey, Nathaniel A., et al.
Publicado: (2020)

A Cell-Free Microtiter Plate Screen for Improved [FeFe] Hydrogenases
por: Stapleton, James A., et al.
Publicado: (2010)

Development of an In Vitro Compartmentalization Screen for High-Throughput Directed Evolution of [FeFe] Hydrogenases
por: Stapleton, James A., et al.
Publicado: (2010)

In Vivo EPR Characterization of Semi‐Synthetic [FeFe] Hydrogenases
por: Mészáros, Lívia S., et al.
Publicado: (2018)

Muonium Chemistry at Diiron Subsite Analogues of [FeFe]‐Hydrogenase
por: Wright, Joseph A., et al.
Publicado: (2016)

Accumulating the hydride state in the catalytic cycle of [FeFe]-hydrogenases
por: Winkler, Martin, et al.
Publicado: (2017)

Synthesis of a miniaturized [FeFe] hydrogenase model system
por: Esmieu, Charlène, et al.
Publicado: (2019)

Spectroscopic and biochemical insight into an electron-bifurcating [FeFe] hydrogenase
por: Chongdar, Nipa, et al.
Publicado: (2019)

How [FeFe]-Hydrogenase Facilitates Bidirectional Proton Transfer
por: Senger, Moritz, et al.
Publicado: (2019)

Structural insight on the mechanism of an electron-bifurcating [FeFe] hydrogenase
por: Furlan, Chris, et al.
Publicado: (2022)

Heteropolymetallic [FeFe]-Hydrogenase Mimics: Synthesis and Electrochemical Properties
por: Torres, Alejandro, et al.
Publicado: (2023)

A synthetic system links FeFe-hydrogenases to essential E. coli sulfur metabolism
por: Barstow, Buz, et al.
Publicado: (2011)

Spectroscopic and Computational Evidence that [FeFe] Hydrogenases Operate Exclusively with CO-Bridged Intermediates
por: Birrell, James A., et al.
Publicado: (2019)

The [FeFe] hydrogenase of Nyctotherus ovalis has a chimeric origin
por: Boxma, Brigitte, et al.
Publicado: (2007)

A structural view of synthetic cofactor integration into [FeFe]-hydrogenases
por: Esselborn, J., et al.
Publicado: (2016)

Crystallographic and spectroscopic assignment of the proton transfer pathway in [FeFe]-hydrogenases
por: Duan, Jifu, et al.
Publicado: (2018)

Discovery of novel [FeFe]-hydrogenases for biocatalytic H(2)-production
por: Land, Henrik, et al.
Publicado: (2019)

The exchange activities of [Fe] hydrogenase (iron–sulfur-cluster-free hydrogenase) from methanogenic archaea in comparison with the exchange activities of [FeFe] and [NiFe] hydrogenases
por: Vogt, Sonja, et al.
Publicado: (2007)

Optimized Expression and Purification for High-Activity Preparations of Algal [FeFe]-Hydrogenase
por: Yacoby, Iftach, et al.
Publicado: (2012)

Comprehensive Phylogenetic Diversity of [FeFe]-Hydrogenase Genes in Termite Gut Microbiota
por: Zheng, Hao, et al.
Publicado: (2013)

Spectroscopical Investigations on the Redox Chemistry of [FeFe]-Hydrogenases in the Presence of Carbon Monoxide
por: Laun, Konstantin, et al.
Publicado: (2018)

Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism
por: Wegelius, Adam, et al.
Publicado: (2018)

The Nonphysiological Reductant Sodium Dithionite and [FeFe] Hydrogenase: Influence on the Enzyme Mechanism
por: Martini, Maria Alessandra, et al.
Publicado: (2021)

The Contribution of Proton-Donor pKa on Reactivity Profiles of [FeFe]-hydrogenases
por: Kisgeropoulos, Effie C., et al.
Publicado: (2022)

Aerobic Damage to [FeFe]-Hydrogenases: Activation Barriers for the Chemical Attachment of O(2)**
por: Kubas, Adam, et al.
Publicado: (2014)

Hydrogen Production Catalyzed by Bidirectional, Biomimetic Models of the [FeFe]-Hydrogenase Active Site
por: Lansing, James C., et al.
Publicado: (2014)

Complex Multimeric [FeFe] Hydrogenases: Biochemistry, Physiology and New Opportunities for the Hydrogen Economy
por: Schuchmann, Kai, et al.
Publicado: (2018)

Activating a [FeFe] Hydrogenase Mimic for Hydrogen Evolution under Visible Light
por: Buday, Philipp, et al.
Publicado: (2022)

Binding of exogenous cyanide reveals new active-site states in [FeFe] hydrogenases
por: Martini, Maria Alessandra, et al.
Publicado: (2023)

Molecular Basis of the Electron Bifurcation Mechanism in the [FeFe]-Hydrogenase Complex HydABC
por: Katsyv, Alexander, et al.
Publicado: (2023)

Cyanide Binding to [FeFe]‐Hydrogenase Stabilizes the Alternative Configuration of the Proton Transfer Pathway
por: Duan, Jifu, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_3c9cu792e7eajfjrtk4e2273vm