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Scaffold Sheet Design Strategy for Soft Tissue Engineering †

Creating heterogeneous tissue constructs with an even cell distribution and robust mechanical strength remain important challenges to the success of in vivo tissue engineering. To address these issues, we are developing a scaffold sheet tissue engineering strategy consisting of thin (~200 μm), stron...

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Detalles Bibliográficos
Autores principales: Tran, Richard T., Thevenot, Paul, Zhang, Yi, Gyawali, Dipendra, Tang, Liping, Yang, Jian
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2992388/
https://www.ncbi.nlm.nih.gov/pubmed/21113339
http://dx.doi.org/10.3390/ma3021375
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author Tran, Richard T.
Thevenot, Paul
Zhang, Yi
Gyawali, Dipendra
Tang, Liping
Yang, Jian
author_facet Tran, Richard T.
Thevenot, Paul
Zhang, Yi
Gyawali, Dipendra
Tang, Liping
Yang, Jian
author_sort Tran, Richard T.
collection PubMed
description Creating heterogeneous tissue constructs with an even cell distribution and robust mechanical strength remain important challenges to the success of in vivo tissue engineering. To address these issues, we are developing a scaffold sheet tissue engineering strategy consisting of thin (~200 μm), strong, elastic, and porous crosslinked urethane-doped polyester (CUPE) scaffold sheets that are bonded together chemically or through cell culture. Suture retention of the tissue constructs (four sheets) fabricated by the scaffold sheet tissue engineering strategy is close to the surgical requirement (1.8 N) rendering their potential for immediate implantation without a need for long cell culture times. Cell culture results using 3T3 fibroblasts show that the scaffold sheets are bonded into a tissue construct via the extracellular matrix produced by the cells after 2 weeks of in vitro cell culture.
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spelling pubmed-29923882010-11-26 Scaffold Sheet Design Strategy for Soft Tissue Engineering † Tran, Richard T. Thevenot, Paul Zhang, Yi Gyawali, Dipendra Tang, Liping Yang, Jian Materials (Basel) Article Creating heterogeneous tissue constructs with an even cell distribution and robust mechanical strength remain important challenges to the success of in vivo tissue engineering. To address these issues, we are developing a scaffold sheet tissue engineering strategy consisting of thin (~200 μm), strong, elastic, and porous crosslinked urethane-doped polyester (CUPE) scaffold sheets that are bonded together chemically or through cell culture. Suture retention of the tissue constructs (four sheets) fabricated by the scaffold sheet tissue engineering strategy is close to the surgical requirement (1.8 N) rendering their potential for immediate implantation without a need for long cell culture times. Cell culture results using 3T3 fibroblasts show that the scaffold sheets are bonded into a tissue construct via the extracellular matrix produced by the cells after 2 weeks of in vitro cell culture. Molecular Diversity Preservation International 2010-02-24 /pmc/articles/PMC2992388/ /pubmed/21113339 http://dx.doi.org/10.3390/ma3021375 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Tran, Richard T.
Thevenot, Paul
Zhang, Yi
Gyawali, Dipendra
Tang, Liping
Yang, Jian
Scaffold Sheet Design Strategy for Soft Tissue Engineering †
title Scaffold Sheet Design Strategy for Soft Tissue Engineering †
title_full Scaffold Sheet Design Strategy for Soft Tissue Engineering †
title_fullStr Scaffold Sheet Design Strategy for Soft Tissue Engineering †
title_full_unstemmed Scaffold Sheet Design Strategy for Soft Tissue Engineering †
title_short Scaffold Sheet Design Strategy for Soft Tissue Engineering †
title_sort scaffold sheet design strategy for soft tissue engineering †
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2992388/
https://www.ncbi.nlm.nih.gov/pubmed/21113339
http://dx.doi.org/10.3390/ma3021375
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