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Scaffold Sheet Design Strategy for Soft Tissue Engineering †
Creating heterogeneous tissue constructs with an even cell distribution and robust mechanical strength remain important challenges to the success of in vivo tissue engineering. To address these issues, we are developing a scaffold sheet tissue engineering strategy consisting of thin (~200 μm), stron...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2992388/ https://www.ncbi.nlm.nih.gov/pubmed/21113339 http://dx.doi.org/10.3390/ma3021375 |
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author | Tran, Richard T. Thevenot, Paul Zhang, Yi Gyawali, Dipendra Tang, Liping Yang, Jian |
author_facet | Tran, Richard T. Thevenot, Paul Zhang, Yi Gyawali, Dipendra Tang, Liping Yang, Jian |
author_sort | Tran, Richard T. |
collection | PubMed |
description | Creating heterogeneous tissue constructs with an even cell distribution and robust mechanical strength remain important challenges to the success of in vivo tissue engineering. To address these issues, we are developing a scaffold sheet tissue engineering strategy consisting of thin (~200 μm), strong, elastic, and porous crosslinked urethane-doped polyester (CUPE) scaffold sheets that are bonded together chemically or through cell culture. Suture retention of the tissue constructs (four sheets) fabricated by the scaffold sheet tissue engineering strategy is close to the surgical requirement (1.8 N) rendering their potential for immediate implantation without a need for long cell culture times. Cell culture results using 3T3 fibroblasts show that the scaffold sheets are bonded into a tissue construct via the extracellular matrix produced by the cells after 2 weeks of in vitro cell culture. |
format | Text |
id | pubmed-2992388 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Diversity Preservation International |
record_format | MEDLINE/PubMed |
spelling | pubmed-29923882010-11-26 Scaffold Sheet Design Strategy for Soft Tissue Engineering † Tran, Richard T. Thevenot, Paul Zhang, Yi Gyawali, Dipendra Tang, Liping Yang, Jian Materials (Basel) Article Creating heterogeneous tissue constructs with an even cell distribution and robust mechanical strength remain important challenges to the success of in vivo tissue engineering. To address these issues, we are developing a scaffold sheet tissue engineering strategy consisting of thin (~200 μm), strong, elastic, and porous crosslinked urethane-doped polyester (CUPE) scaffold sheets that are bonded together chemically or through cell culture. Suture retention of the tissue constructs (four sheets) fabricated by the scaffold sheet tissue engineering strategy is close to the surgical requirement (1.8 N) rendering their potential for immediate implantation without a need for long cell culture times. Cell culture results using 3T3 fibroblasts show that the scaffold sheets are bonded into a tissue construct via the extracellular matrix produced by the cells after 2 weeks of in vitro cell culture. Molecular Diversity Preservation International 2010-02-24 /pmc/articles/PMC2992388/ /pubmed/21113339 http://dx.doi.org/10.3390/ma3021375 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Tran, Richard T. Thevenot, Paul Zhang, Yi Gyawali, Dipendra Tang, Liping Yang, Jian Scaffold Sheet Design Strategy for Soft Tissue Engineering † |
title | Scaffold Sheet Design Strategy for Soft Tissue Engineering † |
title_full | Scaffold Sheet Design Strategy for Soft Tissue Engineering † |
title_fullStr | Scaffold Sheet Design Strategy for Soft Tissue Engineering † |
title_full_unstemmed | Scaffold Sheet Design Strategy for Soft Tissue Engineering † |
title_short | Scaffold Sheet Design Strategy for Soft Tissue Engineering † |
title_sort | scaffold sheet design strategy for soft tissue engineering † |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2992388/ https://www.ncbi.nlm.nih.gov/pubmed/21113339 http://dx.doi.org/10.3390/ma3021375 |
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