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REAP: A two minute cell fractionation method

BACKGROUND: The translocation or shuttling of proteins between the nucleus and cytoplasm (nucleocytoplasmic transport [NCPT]) is often a rapid event following stimulation with growth factors or in response to stress or other experimental manipulations. Commonly used methods to separate nuclei from c...

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Detalles Bibliográficos
Autores principales: Suzuki, Keiko, Bose, Pinaki, Leong-Quong, Rebecca YY, Fujita, Donald J, Riabowol, Karl
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2993727/
https://www.ncbi.nlm.nih.gov/pubmed/21067583
http://dx.doi.org/10.1186/1756-0500-3-294

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