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Root-synthesized cytokinins improve shoot growth and fruit yield in salinized tomato (Solanum lycopersicum L.) plants

Salinity limits crop productivity, in part by decreasing shoot concentrations of the growth-promoting and senescence-delaying hormones cytokinins. Since constitutive cytokinin overproduction may have pleiotropic effects on plant development, two approaches assessed whether specific root-localized tr...

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Autores principales: Ghanem, Michel Edmond, Albacete, Alfonso, Smigocki, Ann C., Frébort, Ivo, Pospíšilová, Hana, Martínez-Andújar, Cristina, Acosta, Manuel, Sánchez-Bravo, José, Lutts, Stanley, Dodd, Ian C., Pérez-Alfocea, Francisco
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2993914/
https://www.ncbi.nlm.nih.gov/pubmed/20959628
http://dx.doi.org/10.1093/jxb/erq266
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author Ghanem, Michel Edmond
Albacete, Alfonso
Smigocki, Ann C.
Frébort, Ivo
Pospíšilová, Hana
Martínez-Andújar, Cristina
Acosta, Manuel
Sánchez-Bravo, José
Lutts, Stanley
Dodd, Ian C.
Pérez-Alfocea, Francisco
author_facet Ghanem, Michel Edmond
Albacete, Alfonso
Smigocki, Ann C.
Frébort, Ivo
Pospíšilová, Hana
Martínez-Andújar, Cristina
Acosta, Manuel
Sánchez-Bravo, José
Lutts, Stanley
Dodd, Ian C.
Pérez-Alfocea, Francisco
author_sort Ghanem, Michel Edmond
collection PubMed
description Salinity limits crop productivity, in part by decreasing shoot concentrations of the growth-promoting and senescence-delaying hormones cytokinins. Since constitutive cytokinin overproduction may have pleiotropic effects on plant development, two approaches assessed whether specific root-localized transgenic IPT (a key enzyme for cytokinin biosynthesis) gene expression could substantially improve tomato plant growth and yield under salinity: transient root IPT induction (HSP70::IPT) and grafting wild-type (WT) shoots onto a constitutive IPT-expressing rootstock (WT/35S::IPT). Transient root IPT induction increased root, xylem sap, and leaf bioactive cytokinin concentrations 2- to 3-fold without shoot IPT gene expression. Although IPT induction reduced root biomass (by 15%) in control (non-salinized) plants, in salinized plants (100 mM NaCl for 22 d), increased cytokinin concentrations delayed stomatal closure and leaf senescence and almost doubled shoot growth (compared with WT plants), with concomitant increases in the essential nutrient K(+) (20%) and decreases in the toxic ion Na(+) (by 30%) and abscisic acid (by 20–40%) concentrations in transpiring mature leaves. Similarly, WT/35S::IPT plants (scion/rootstock) grown with 75 mM NaCl for 90 d had higher fruit trans-zeatin concentrations (1.5- to 2-fold) and yielded 30% more than WT/non-transformed plants. Enhancing root cytokinin synthesis modified both shoot hormonal and ionic status, thus ameliorating salinity-induced decreases in growth and yield.
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spelling pubmed-29939142010-12-01 Root-synthesized cytokinins improve shoot growth and fruit yield in salinized tomato (Solanum lycopersicum L.) plants Ghanem, Michel Edmond Albacete, Alfonso Smigocki, Ann C. Frébort, Ivo Pospíšilová, Hana Martínez-Andújar, Cristina Acosta, Manuel Sánchez-Bravo, José Lutts, Stanley Dodd, Ian C. Pérez-Alfocea, Francisco J Exp Bot Research Papers Salinity limits crop productivity, in part by decreasing shoot concentrations of the growth-promoting and senescence-delaying hormones cytokinins. Since constitutive cytokinin overproduction may have pleiotropic effects on plant development, two approaches assessed whether specific root-localized transgenic IPT (a key enzyme for cytokinin biosynthesis) gene expression could substantially improve tomato plant growth and yield under salinity: transient root IPT induction (HSP70::IPT) and grafting wild-type (WT) shoots onto a constitutive IPT-expressing rootstock (WT/35S::IPT). Transient root IPT induction increased root, xylem sap, and leaf bioactive cytokinin concentrations 2- to 3-fold without shoot IPT gene expression. Although IPT induction reduced root biomass (by 15%) in control (non-salinized) plants, in salinized plants (100 mM NaCl for 22 d), increased cytokinin concentrations delayed stomatal closure and leaf senescence and almost doubled shoot growth (compared with WT plants), with concomitant increases in the essential nutrient K(+) (20%) and decreases in the toxic ion Na(+) (by 30%) and abscisic acid (by 20–40%) concentrations in transpiring mature leaves. Similarly, WT/35S::IPT plants (scion/rootstock) grown with 75 mM NaCl for 90 d had higher fruit trans-zeatin concentrations (1.5- to 2-fold) and yielded 30% more than WT/non-transformed plants. Enhancing root cytokinin synthesis modified both shoot hormonal and ionic status, thus ameliorating salinity-induced decreases in growth and yield. Oxford University Press 2011-01 2010-10-19 /pmc/articles/PMC2993914/ /pubmed/20959628 http://dx.doi.org/10.1093/jxb/erq266 Text en © 2010 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Papers
Ghanem, Michel Edmond
Albacete, Alfonso
Smigocki, Ann C.
Frébort, Ivo
Pospíšilová, Hana
Martínez-Andújar, Cristina
Acosta, Manuel
Sánchez-Bravo, José
Lutts, Stanley
Dodd, Ian C.
Pérez-Alfocea, Francisco
Root-synthesized cytokinins improve shoot growth and fruit yield in salinized tomato (Solanum lycopersicum L.) plants
title Root-synthesized cytokinins improve shoot growth and fruit yield in salinized tomato (Solanum lycopersicum L.) plants
title_full Root-synthesized cytokinins improve shoot growth and fruit yield in salinized tomato (Solanum lycopersicum L.) plants
title_fullStr Root-synthesized cytokinins improve shoot growth and fruit yield in salinized tomato (Solanum lycopersicum L.) plants
title_full_unstemmed Root-synthesized cytokinins improve shoot growth and fruit yield in salinized tomato (Solanum lycopersicum L.) plants
title_short Root-synthesized cytokinins improve shoot growth and fruit yield in salinized tomato (Solanum lycopersicum L.) plants
title_sort root-synthesized cytokinins improve shoot growth and fruit yield in salinized tomato (solanum lycopersicum l.) plants
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2993914/
https://www.ncbi.nlm.nih.gov/pubmed/20959628
http://dx.doi.org/10.1093/jxb/erq266
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