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Flowers of Inula japonica Attenuate Inflammatory Responses

BACKGROUND: The flowers of Inula japonica (Inulae Flos) have long been used in traditional medicine for the treatment of inflammatory diseases. In the present study, we investigated the anti-inflammatory properties of Inulae Flos Extract (IFE). METHODS: The anti-inflammatory effects of IFE against n...

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Autores principales: Choi, Jeon Hyeun, Park, Young Na, Li, Ying, Jin, Mei Hua, Lee, Jiean, Lee, Younju, Son, Jong Keun, Chang, Hyeun Wook, Lee, Eunkyung
Formato: Texto
Lenguaje:English
Publicado: The Korean Association of Immunologists 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2993946/
https://www.ncbi.nlm.nih.gov/pubmed/21165243
http://dx.doi.org/10.4110/in.2010.10.5.145
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author Choi, Jeon Hyeun
Park, Young Na
Li, Ying
Jin, Mei Hua
Lee, Jiean
Lee, Younju
Son, Jong Keun
Chang, Hyeun Wook
Lee, Eunkyung
author_facet Choi, Jeon Hyeun
Park, Young Na
Li, Ying
Jin, Mei Hua
Lee, Jiean
Lee, Younju
Son, Jong Keun
Chang, Hyeun Wook
Lee, Eunkyung
author_sort Choi, Jeon Hyeun
collection PubMed
description BACKGROUND: The flowers of Inula japonica (Inulae Flos) have long been used in traditional medicine for the treatment of inflammatory diseases. In the present study, we investigated the anti-inflammatory properties of Inulae Flos Extract (IFE). METHODS: The anti-inflammatory effects of IFE against nitric oxide (NO), PGE(2), TNF-α, and IL-6 release, as well as NF-κB and MAP kinase activation were evaluated in RAW 264.7 cells. RESULTS: IFE inhibited the production of NO and the expression of inducible nitric oxide synthase (iNOS) in LPS-stimulated RAW264.7 cells. In addition, IFE reduced the release of pro-inflammatory cytokines, such as TNF-α and IL-6. Furthermore, IFE inhibited the NF-κB activation induced by LPS, which was associated with the abrogation of IκB-α degradation and subsequent decreases in nuclear p65 and p50 levels. Moreover, the phosphorylation of ERK, JNK, and p38 MAP kinases in LPS-stimulated RAW 264.7 cells was suppressed by IFE in a dose-dependent manner. CONCLUSION: These results suggest that the anti-inflammation activities of IFE might be attributed to the inhibition of NO, iNOS and cytokine expression through the down-regulation of NF-κB activation via suppression of IκBα and MAP kinase phosphorylation in macrophages.
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spelling pubmed-29939462010-12-16 Flowers of Inula japonica Attenuate Inflammatory Responses Choi, Jeon Hyeun Park, Young Na Li, Ying Jin, Mei Hua Lee, Jiean Lee, Younju Son, Jong Keun Chang, Hyeun Wook Lee, Eunkyung Immune Netw Original Article BACKGROUND: The flowers of Inula japonica (Inulae Flos) have long been used in traditional medicine for the treatment of inflammatory diseases. In the present study, we investigated the anti-inflammatory properties of Inulae Flos Extract (IFE). METHODS: The anti-inflammatory effects of IFE against nitric oxide (NO), PGE(2), TNF-α, and IL-6 release, as well as NF-κB and MAP kinase activation were evaluated in RAW 264.7 cells. RESULTS: IFE inhibited the production of NO and the expression of inducible nitric oxide synthase (iNOS) in LPS-stimulated RAW264.7 cells. In addition, IFE reduced the release of pro-inflammatory cytokines, such as TNF-α and IL-6. Furthermore, IFE inhibited the NF-κB activation induced by LPS, which was associated with the abrogation of IκB-α degradation and subsequent decreases in nuclear p65 and p50 levels. Moreover, the phosphorylation of ERK, JNK, and p38 MAP kinases in LPS-stimulated RAW 264.7 cells was suppressed by IFE in a dose-dependent manner. CONCLUSION: These results suggest that the anti-inflammation activities of IFE might be attributed to the inhibition of NO, iNOS and cytokine expression through the down-regulation of NF-κB activation via suppression of IκBα and MAP kinase phosphorylation in macrophages. The Korean Association of Immunologists 2010-10 2010-10-31 /pmc/articles/PMC2993946/ /pubmed/21165243 http://dx.doi.org/10.4110/in.2010.10.5.145 Text en Copyright © 2010 The Korean Association of Immunologists http://creativecommons.org/licenses/by-nc/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Choi, Jeon Hyeun
Park, Young Na
Li, Ying
Jin, Mei Hua
Lee, Jiean
Lee, Younju
Son, Jong Keun
Chang, Hyeun Wook
Lee, Eunkyung
Flowers of Inula japonica Attenuate Inflammatory Responses
title Flowers of Inula japonica Attenuate Inflammatory Responses
title_full Flowers of Inula japonica Attenuate Inflammatory Responses
title_fullStr Flowers of Inula japonica Attenuate Inflammatory Responses
title_full_unstemmed Flowers of Inula japonica Attenuate Inflammatory Responses
title_short Flowers of Inula japonica Attenuate Inflammatory Responses
title_sort flowers of inula japonica attenuate inflammatory responses
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2993946/
https://www.ncbi.nlm.nih.gov/pubmed/21165243
http://dx.doi.org/10.4110/in.2010.10.5.145
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