Single-cell qPCR on dispersed primary pituitary cells -an optimized protocol

BACKGROUND: The incidence of false positives is a potential problem in single-cell PCR experiments. This paper describes an optimized protocol for single-cell qPCR measurements in primary pituitary cell cultures following patch-clamp recordings. Two different cell harvesting methods were assessed us...

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Detalles Bibliográficos
Autores principales: Hodne, Kjetil, Haug, Trude M, Weltzien, Finn-Arne
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2994858/
https://www.ncbi.nlm.nih.gov/pubmed/21073722
http://dx.doi.org/10.1186/1471-2199-11-82

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2994858/
https://www.ncbi.nlm.nih.gov/pubmed/21073722
http://dx.doi.org/10.1186/1471-2199-11-82

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