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Single-cell qPCR on dispersed primary pituitary cells -an optimized protocol
BACKGROUND: The incidence of false positives is a potential problem in single-cell PCR experiments. This paper describes an optimized protocol for single-cell qPCR measurements in primary pituitary cell cultures following patch-clamp recordings. Two different cell harvesting methods were assessed us...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2994858/ https://www.ncbi.nlm.nih.gov/pubmed/21073722 http://dx.doi.org/10.1186/1471-2199-11-82 |