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Efficient assembly of very short oligonucleotides using T4 DNA Ligase

BACKGROUND: In principle, a pre-constructed library of all possible short oligonucleotides could be used to construct many distinct gene sequences. In order to assess the feasibility of such an approach, we characterized T4 DNA Ligase activity on short oligonucleotide substrates and defined conditio...

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Detalles Bibliográficos
Autores principales:	Horspool, Daniel R, Coope, Robin JN, Holt, Robert A
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2010
Materias:	Short Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2994885/ https://www.ncbi.nlm.nih.gov/pubmed/21062485 http://dx.doi.org/10.1186/1756-0500-3-291

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