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IP-10 detection in urine is associated with lung diseases

BACKGROUND: blood cytokines and chemokines have been proposed as biomarkers for tuberculosis (TB). Recently, some immune mediators found in the urine of patients with renal dysfunctions have also been suggested as potential biomarkers. Finding biomarkers for TB in urine would present several advanta...

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Autores principales: Cannas, Angela, Calvo, Ludovica, Chiacchio, Teresa, Cuzzi, Gilda, Vanini, Valentina, Lauria, Francesco N, Pucci, Luigia, Girardi, Enrico, Goletti, Delia
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2995466/
https://www.ncbi.nlm.nih.gov/pubmed/21092156
http://dx.doi.org/10.1186/1471-2334-10-333
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author Cannas, Angela
Calvo, Ludovica
Chiacchio, Teresa
Cuzzi, Gilda
Vanini, Valentina
Lauria, Francesco N
Pucci, Luigia
Girardi, Enrico
Goletti, Delia
author_facet Cannas, Angela
Calvo, Ludovica
Chiacchio, Teresa
Cuzzi, Gilda
Vanini, Valentina
Lauria, Francesco N
Pucci, Luigia
Girardi, Enrico
Goletti, Delia
author_sort Cannas, Angela
collection PubMed
description BACKGROUND: blood cytokines and chemokines have been proposed as biomarkers for tuberculosis (TB). Recently, some immune mediators found in the urine of patients with renal dysfunctions have also been suggested as potential biomarkers. Finding biomarkers for TB in urine would present several advantages over blood in terms of collection and safety. The objective of this study was to investigate the presence of cytokines and chemokines in the urine of patients with pulmonary TB at the time of diagnosis. In a subgroup, the evaluation was also performed during TB treatment and at therapy completion. Patients with lung diseases other than TB, and healthy subjects were also enrolled. METHODS: urine samples from 138 individuals, after exclusion of renal dysfunctions, were collected during an 18 month-period. Among them, 58 received a diagnosis of pulmonary TB, 28 resulted having lung diseases other than TB, and 34 were healthy subjects. Moreover, 18 TB patients, 9 of whom were tested 2 months after AFB smear sputum reversion and 9 of whom were cured of TB were also included. Cytokines and chemokines in urine were evaluated using a Cytometric-Bead-Array-Flex-Set. IP-10 detection in 49 subjects was also carried out in parallel by using an Enzyme Linked ImmunoSorbent Assay (ELISA). RESULTS: IFN-γ, TNF-α, IL-2, IL-8, MIP-1α, MIP-1β and RANTES were poorly detected in all urine samples. Conversely, IP-10 was consistently detected in urine and its level was significantly increased in patients with lung disease compared to healthy subjects (p < 0.001). Increased IP-10 levels were found in both pulmonary TB and lung diseases other than TB. Moreover lower IP-10 levels were found in cured-TB patients compared to the levels at the time of diagnosis, and this difference was close to significance (p = 0.06). Interestingly, we demonstrated a significant correlation between the data obtained by flow cytometry and ELISA (r(2 )0.82, p < 0.0001). CONCLUSIONS: IP-10, in contrast to IFN-γ, TNF-α, IL-2, IL-8, MIP-1α, MIP-1β and RANTES, is detectable in the urine of patients with pulmonary diseases in the absence of renal dysfunctions. Moreover, the IP-10 level in cured-TB patients is comparable to that found in healthy subjects. More studies are needed to further investigate the clinical utility of these findings.
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spelling pubmed-29954662010-12-02 IP-10 detection in urine is associated with lung diseases Cannas, Angela Calvo, Ludovica Chiacchio, Teresa Cuzzi, Gilda Vanini, Valentina Lauria, Francesco N Pucci, Luigia Girardi, Enrico Goletti, Delia BMC Infect Dis Research Article BACKGROUND: blood cytokines and chemokines have been proposed as biomarkers for tuberculosis (TB). Recently, some immune mediators found in the urine of patients with renal dysfunctions have also been suggested as potential biomarkers. Finding biomarkers for TB in urine would present several advantages over blood in terms of collection and safety. The objective of this study was to investigate the presence of cytokines and chemokines in the urine of patients with pulmonary TB at the time of diagnosis. In a subgroup, the evaluation was also performed during TB treatment and at therapy completion. Patients with lung diseases other than TB, and healthy subjects were also enrolled. METHODS: urine samples from 138 individuals, after exclusion of renal dysfunctions, were collected during an 18 month-period. Among them, 58 received a diagnosis of pulmonary TB, 28 resulted having lung diseases other than TB, and 34 were healthy subjects. Moreover, 18 TB patients, 9 of whom were tested 2 months after AFB smear sputum reversion and 9 of whom were cured of TB were also included. Cytokines and chemokines in urine were evaluated using a Cytometric-Bead-Array-Flex-Set. IP-10 detection in 49 subjects was also carried out in parallel by using an Enzyme Linked ImmunoSorbent Assay (ELISA). RESULTS: IFN-γ, TNF-α, IL-2, IL-8, MIP-1α, MIP-1β and RANTES were poorly detected in all urine samples. Conversely, IP-10 was consistently detected in urine and its level was significantly increased in patients with lung disease compared to healthy subjects (p < 0.001). Increased IP-10 levels were found in both pulmonary TB and lung diseases other than TB. Moreover lower IP-10 levels were found in cured-TB patients compared to the levels at the time of diagnosis, and this difference was close to significance (p = 0.06). Interestingly, we demonstrated a significant correlation between the data obtained by flow cytometry and ELISA (r(2 )0.82, p < 0.0001). CONCLUSIONS: IP-10, in contrast to IFN-γ, TNF-α, IL-2, IL-8, MIP-1α, MIP-1β and RANTES, is detectable in the urine of patients with pulmonary diseases in the absence of renal dysfunctions. Moreover, the IP-10 level in cured-TB patients is comparable to that found in healthy subjects. More studies are needed to further investigate the clinical utility of these findings. BioMed Central 2010-11-22 /pmc/articles/PMC2995466/ /pubmed/21092156 http://dx.doi.org/10.1186/1471-2334-10-333 Text en Copyright ©2010 Cannas et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cannas, Angela
Calvo, Ludovica
Chiacchio, Teresa
Cuzzi, Gilda
Vanini, Valentina
Lauria, Francesco N
Pucci, Luigia
Girardi, Enrico
Goletti, Delia
IP-10 detection in urine is associated with lung diseases
title IP-10 detection in urine is associated with lung diseases
title_full IP-10 detection in urine is associated with lung diseases
title_fullStr IP-10 detection in urine is associated with lung diseases
title_full_unstemmed IP-10 detection in urine is associated with lung diseases
title_short IP-10 detection in urine is associated with lung diseases
title_sort ip-10 detection in urine is associated with lung diseases
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2995466/
https://www.ncbi.nlm.nih.gov/pubmed/21092156
http://dx.doi.org/10.1186/1471-2334-10-333
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