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Response to Dengue virus infections altered by cytokine-like substances from mosquito cell cultures

BACKGROUND: With both shrimp and commercial insects such as honey bees, it is known that stable, persistent viral infections characterized by absence of disease can sometimes shift to overt disease states as a result of various stress triggers and that this can result in serious economic losses. The...

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Autores principales: Kanthong, Nipaporn, Laosutthipong, Chaowanee, Flegel, Timothy W
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2995469/
https://www.ncbi.nlm.nih.gov/pubmed/21078201
http://dx.doi.org/10.1186/1471-2180-10-290
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author Kanthong, Nipaporn
Laosutthipong, Chaowanee
Flegel, Timothy W
author_facet Kanthong, Nipaporn
Laosutthipong, Chaowanee
Flegel, Timothy W
author_sort Kanthong, Nipaporn
collection PubMed
description BACKGROUND: With both shrimp and commercial insects such as honey bees, it is known that stable, persistent viral infections characterized by absence of disease can sometimes shift to overt disease states as a result of various stress triggers and that this can result in serious economic losses. The main research interest of our group is to understand the dynamics of stable viral infections in shrimp and how they can be destabilized by stress. Since there are no continuous cell lines for crustaceans, we have used a C6/36 mosquito cell line infected with Dengue virus to test hypotheses regarding these interactions. As a result, we accidentally discovered two new cytokine-like substances in 5 kDa extracts from supernatant solutions of acutely and persistently infected mosquito cells. RESULTS: Naïve C6/36 cells were exposed for 48 h to 5 kDa membrane filtrates prepared from the supernatant medium of stable C6/36 mosquito cell cultures persistently-infected with Dengue virus. Subsequent challenge of naïve cells with a virulent stock of Dengue virus 2 (DEN-2) and analysis by confocal immunofluorescence microscopy using anti-DEN-2 antibody revealed a dramatic reduction in the percentage of DEN-2 infected cells when compared to control cells. Similar filtrates prepared from C6/36 cells with acute DEN-2 infections were used to treat stable C6/36 mosquito cell cultures persistently-infected with Dengue virus. Confocal immunofluorescence microscopy revealed destabilization in the form of an apoptosis-like response. Proteinase K treatment removed the cell-altering activities indicating that they were caused by small polypeptides similar to those previously reported from insects. CONCLUSIONS: This is the first report of cytokine-like substances that can alter the responses of mosquito cells to Dengue virus. This simple model system allows detailed molecular studies on insect cytokine production and on cytokine activity in a standard insect cell line.
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spelling pubmed-29954692010-12-02 Response to Dengue virus infections altered by cytokine-like substances from mosquito cell cultures Kanthong, Nipaporn Laosutthipong, Chaowanee Flegel, Timothy W BMC Microbiol Research Article BACKGROUND: With both shrimp and commercial insects such as honey bees, it is known that stable, persistent viral infections characterized by absence of disease can sometimes shift to overt disease states as a result of various stress triggers and that this can result in serious economic losses. The main research interest of our group is to understand the dynamics of stable viral infections in shrimp and how they can be destabilized by stress. Since there are no continuous cell lines for crustaceans, we have used a C6/36 mosquito cell line infected with Dengue virus to test hypotheses regarding these interactions. As a result, we accidentally discovered two new cytokine-like substances in 5 kDa extracts from supernatant solutions of acutely and persistently infected mosquito cells. RESULTS: Naïve C6/36 cells were exposed for 48 h to 5 kDa membrane filtrates prepared from the supernatant medium of stable C6/36 mosquito cell cultures persistently-infected with Dengue virus. Subsequent challenge of naïve cells with a virulent stock of Dengue virus 2 (DEN-2) and analysis by confocal immunofluorescence microscopy using anti-DEN-2 antibody revealed a dramatic reduction in the percentage of DEN-2 infected cells when compared to control cells. Similar filtrates prepared from C6/36 cells with acute DEN-2 infections were used to treat stable C6/36 mosquito cell cultures persistently-infected with Dengue virus. Confocal immunofluorescence microscopy revealed destabilization in the form of an apoptosis-like response. Proteinase K treatment removed the cell-altering activities indicating that they were caused by small polypeptides similar to those previously reported from insects. CONCLUSIONS: This is the first report of cytokine-like substances that can alter the responses of mosquito cells to Dengue virus. This simple model system allows detailed molecular studies on insect cytokine production and on cytokine activity in a standard insect cell line. BioMed Central 2010-11-16 /pmc/articles/PMC2995469/ /pubmed/21078201 http://dx.doi.org/10.1186/1471-2180-10-290 Text en Copyright ©2010 Kanthong et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kanthong, Nipaporn
Laosutthipong, Chaowanee
Flegel, Timothy W
Response to Dengue virus infections altered by cytokine-like substances from mosquito cell cultures
title Response to Dengue virus infections altered by cytokine-like substances from mosquito cell cultures
title_full Response to Dengue virus infections altered by cytokine-like substances from mosquito cell cultures
title_fullStr Response to Dengue virus infections altered by cytokine-like substances from mosquito cell cultures
title_full_unstemmed Response to Dengue virus infections altered by cytokine-like substances from mosquito cell cultures
title_short Response to Dengue virus infections altered by cytokine-like substances from mosquito cell cultures
title_sort response to dengue virus infections altered by cytokine-like substances from mosquito cell cultures
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2995469/
https://www.ncbi.nlm.nih.gov/pubmed/21078201
http://dx.doi.org/10.1186/1471-2180-10-290
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