A streamlined sub-cloning procedure to transfer shRNA from a pSM2 vector to a pGIPZ lentiviral vector

RNA interference (RNAi) is a widely used molecular biology technique to investigate the importance of specific genes in molecular pathways. Since mammalian cells are equipped with endogenous RNAi processing machinery, it has become common practice to transfect constructs that encode for short hairpi...

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Detalles Bibliográficos
Autores principales: Ansaloni, Sara, Lelkes, Nadav, Snyder, Jonathan, Epstein, Charles, Dubey, Aditi, Saunders, Aleister J
Formato: Texto
Lenguaje:English
Publicado: Library Publishing Media 2010
Materias:
Technology Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2995545/
https://www.ncbi.nlm.nih.gov/pubmed/21139985

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