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Short tandem repeat profiling: part of an overall strategy for reducing the frequency of cell misidentification

The role of cell authentication in biomedical science has received considerable attention, especially within the past decade. This quality control attribute is now beginning to be given the emphasis it deserves by granting agencies and by scientific journals. Short tandem repeat (STR) profiling, one...

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Detalles Bibliográficos
Autores principales: Nims, Raymond W., Sykes, Greg, Cottrill, Karin, Ikonomi, Pranvera, Elmore, Eugene
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2995877/
https://www.ncbi.nlm.nih.gov/pubmed/20927602
http://dx.doi.org/10.1007/s11626-010-9352-9
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author Nims, Raymond W.
Sykes, Greg
Cottrill, Karin
Ikonomi, Pranvera
Elmore, Eugene
author_facet Nims, Raymond W.
Sykes, Greg
Cottrill, Karin
Ikonomi, Pranvera
Elmore, Eugene
author_sort Nims, Raymond W.
collection PubMed
description The role of cell authentication in biomedical science has received considerable attention, especially within the past decade. This quality control attribute is now beginning to be given the emphasis it deserves by granting agencies and by scientific journals. Short tandem repeat (STR) profiling, one of a few DNA profiling technologies now available, is being proposed for routine identification (authentication) of human cell lines, stem cells, and tissues. The advantage of this technique over methods such as isoenzyme analysis, karyotyping, human leukocyte antigen typing, etc., is that STR profiling can establish identity to the individual level, provided that the appropriate number and types of loci are evaluated. To best employ this technology, a standardized protocol and a data-driven, quality-controlled, and publically searchable database will be necessary. This public STR database (currently under development) will enable investigators to rapidly authenticate human-based cultures to the individual from whom the cells were sourced. Use of similar approaches for non-human animal cells will require developing other suitable loci sets. While implementing STR analysis on a more routine basis should significantly reduce the frequency of cell misidentification, additional technologies may be needed as part of an overall authentication paradigm. For instance, isoenzyme analysis, PCR-based DNA amplification, and sequence-based barcoding methods enable rapid confirmation of a cell line’s species of origin while screening against cross-contaminations, especially when the cells present are not recognized by the species-specific STR method. Karyotyping may also be needed as a supporting tool during establishment of an STR database. Finally, good cell culture practices must always remain a major component of any effort to reduce the frequency of cell misidentification.
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spelling pubmed-29958772011-01-04 Short tandem repeat profiling: part of an overall strategy for reducing the frequency of cell misidentification Nims, Raymond W. Sykes, Greg Cottrill, Karin Ikonomi, Pranvera Elmore, Eugene In Vitro Cell Dev Biol Anim Invited Review The role of cell authentication in biomedical science has received considerable attention, especially within the past decade. This quality control attribute is now beginning to be given the emphasis it deserves by granting agencies and by scientific journals. Short tandem repeat (STR) profiling, one of a few DNA profiling technologies now available, is being proposed for routine identification (authentication) of human cell lines, stem cells, and tissues. The advantage of this technique over methods such as isoenzyme analysis, karyotyping, human leukocyte antigen typing, etc., is that STR profiling can establish identity to the individual level, provided that the appropriate number and types of loci are evaluated. To best employ this technology, a standardized protocol and a data-driven, quality-controlled, and publically searchable database will be necessary. This public STR database (currently under development) will enable investigators to rapidly authenticate human-based cultures to the individual from whom the cells were sourced. Use of similar approaches for non-human animal cells will require developing other suitable loci sets. While implementing STR analysis on a more routine basis should significantly reduce the frequency of cell misidentification, additional technologies may be needed as part of an overall authentication paradigm. For instance, isoenzyme analysis, PCR-based DNA amplification, and sequence-based barcoding methods enable rapid confirmation of a cell line’s species of origin while screening against cross-contaminations, especially when the cells present are not recognized by the species-specific STR method. Karyotyping may also be needed as a supporting tool during establishment of an STR database. Finally, good cell culture practices must always remain a major component of any effort to reduce the frequency of cell misidentification. Springer-Verlag 2010-10-07 2010 /pmc/articles/PMC2995877/ /pubmed/20927602 http://dx.doi.org/10.1007/s11626-010-9352-9 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Invited Review
Nims, Raymond W.
Sykes, Greg
Cottrill, Karin
Ikonomi, Pranvera
Elmore, Eugene
Short tandem repeat profiling: part of an overall strategy for reducing the frequency of cell misidentification
title Short tandem repeat profiling: part of an overall strategy for reducing the frequency of cell misidentification
title_full Short tandem repeat profiling: part of an overall strategy for reducing the frequency of cell misidentification
title_fullStr Short tandem repeat profiling: part of an overall strategy for reducing the frequency of cell misidentification
title_full_unstemmed Short tandem repeat profiling: part of an overall strategy for reducing the frequency of cell misidentification
title_short Short tandem repeat profiling: part of an overall strategy for reducing the frequency of cell misidentification
title_sort short tandem repeat profiling: part of an overall strategy for reducing the frequency of cell misidentification
topic Invited Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2995877/
https://www.ncbi.nlm.nih.gov/pubmed/20927602
http://dx.doi.org/10.1007/s11626-010-9352-9
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