Cargando…

First evaluation of real-time nitric oxide changes in the coronary circulation in patients with non-ischaemic dilated cardiomyopathy using a catheter-type sensor

AIMS: No direct method has yet been developed to measure real-time plasma nitric oxide (NO) concentration in humans. In this study, we evaluated a new method for measuring plasma NO concentration in patients with dilated cardiomyopathy (DCM) and in normal controls using a catheter-type sensor. METHO...

Descripción completa

Detalles Bibliográficos
Autores principales: Takarada, Shigeho, Imanishi, Toshio, Goto, Masami, Mochizuki, Seiichi, Ikejima, Hideyuki, Tsujioka, Hiroto, Kuroi, Akio, Takeshita, Tatsuya, Akasaka, Takashi
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2995954/
https://www.ncbi.nlm.nih.gov/pubmed/20511328
http://dx.doi.org/10.1093/eurheartj/ehq156
_version_ 1782193134144323584
author Takarada, Shigeho
Imanishi, Toshio
Goto, Masami
Mochizuki, Seiichi
Ikejima, Hideyuki
Tsujioka, Hiroto
Kuroi, Akio
Takeshita, Tatsuya
Akasaka, Takashi
author_facet Takarada, Shigeho
Imanishi, Toshio
Goto, Masami
Mochizuki, Seiichi
Ikejima, Hideyuki
Tsujioka, Hiroto
Kuroi, Akio
Takeshita, Tatsuya
Akasaka, Takashi
author_sort Takarada, Shigeho
collection PubMed
description AIMS: No direct method has yet been developed to measure real-time plasma nitric oxide (NO) concentration in humans. In this study, we evaluated a new method for measuring plasma NO concentration in patients with dilated cardiomyopathy (DCM) and in normal controls using a catheter-type sensor. METHODS AND RESULTS: We simultaneously measured average peak velocity (APV) of the coronary artery flow and change in plasma NO concentration using the NO sensor placed in the great cardiac vein of 10 DCM patients and 10 control subjects. These evaluations were performed in response to sequential intracoronary infusions of acetylcholine (ACh, 10(−8)–10(−6) M), N(G)-monomethyl-l-arginine (l-NMMA, 200 µmol) and co-infusion of ACh and l-NMMA. The change in plasma NO concentration in DCM patients was significantly impaired compared with the control group (P < 0.01). Pretreatment with l-NMMA completely suppressed the ACh-induced NO concentration, whereas APV in the left anterior descending coronary artery was partially suppressed in both groups. Plasma NO concentration reached its peak value later than the maximum APV following the injection of ACh (10(−6) M) in both groups. CONCLUSION: The catheter-type NO sensor could be applied to clinically evaluate the endothelial function (i.e. reduced endothelium-derived NO bioavailability) in patients with cardiovascular diseases.
format Text
id pubmed-2995954
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-29959542010-12-06 First evaluation of real-time nitric oxide changes in the coronary circulation in patients with non-ischaemic dilated cardiomyopathy using a catheter-type sensor Takarada, Shigeho Imanishi, Toshio Goto, Masami Mochizuki, Seiichi Ikejima, Hideyuki Tsujioka, Hiroto Kuroi, Akio Takeshita, Tatsuya Akasaka, Takashi Eur Heart J Clinical Research AIMS: No direct method has yet been developed to measure real-time plasma nitric oxide (NO) concentration in humans. In this study, we evaluated a new method for measuring plasma NO concentration in patients with dilated cardiomyopathy (DCM) and in normal controls using a catheter-type sensor. METHODS AND RESULTS: We simultaneously measured average peak velocity (APV) of the coronary artery flow and change in plasma NO concentration using the NO sensor placed in the great cardiac vein of 10 DCM patients and 10 control subjects. These evaluations were performed in response to sequential intracoronary infusions of acetylcholine (ACh, 10(−8)–10(−6) M), N(G)-monomethyl-l-arginine (l-NMMA, 200 µmol) and co-infusion of ACh and l-NMMA. The change in plasma NO concentration in DCM patients was significantly impaired compared with the control group (P < 0.01). Pretreatment with l-NMMA completely suppressed the ACh-induced NO concentration, whereas APV in the left anterior descending coronary artery was partially suppressed in both groups. Plasma NO concentration reached its peak value later than the maximum APV following the injection of ACh (10(−6) M) in both groups. CONCLUSION: The catheter-type NO sensor could be applied to clinically evaluate the endothelial function (i.e. reduced endothelium-derived NO bioavailability) in patients with cardiovascular diseases. Oxford University Press 2010-12 2010-05-28 /pmc/articles/PMC2995954/ /pubmed/20511328 http://dx.doi.org/10.1093/eurheartj/ehq156 Text en Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2010. For permissions please email: journals.permissions@oxfordjournals.org http://creativecommons.org/licenses/by-nc/2.5/ The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that the original authorship is properly and fully attributed; the Journal, Learned Society and Oxford University Press are attributed as the original place of publication with correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org.
spellingShingle Clinical Research
Takarada, Shigeho
Imanishi, Toshio
Goto, Masami
Mochizuki, Seiichi
Ikejima, Hideyuki
Tsujioka, Hiroto
Kuroi, Akio
Takeshita, Tatsuya
Akasaka, Takashi
First evaluation of real-time nitric oxide changes in the coronary circulation in patients with non-ischaemic dilated cardiomyopathy using a catheter-type sensor
title First evaluation of real-time nitric oxide changes in the coronary circulation in patients with non-ischaemic dilated cardiomyopathy using a catheter-type sensor
title_full First evaluation of real-time nitric oxide changes in the coronary circulation in patients with non-ischaemic dilated cardiomyopathy using a catheter-type sensor
title_fullStr First evaluation of real-time nitric oxide changes in the coronary circulation in patients with non-ischaemic dilated cardiomyopathy using a catheter-type sensor
title_full_unstemmed First evaluation of real-time nitric oxide changes in the coronary circulation in patients with non-ischaemic dilated cardiomyopathy using a catheter-type sensor
title_short First evaluation of real-time nitric oxide changes in the coronary circulation in patients with non-ischaemic dilated cardiomyopathy using a catheter-type sensor
title_sort first evaluation of real-time nitric oxide changes in the coronary circulation in patients with non-ischaemic dilated cardiomyopathy using a catheter-type sensor
topic Clinical Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2995954/
https://www.ncbi.nlm.nih.gov/pubmed/20511328
http://dx.doi.org/10.1093/eurheartj/ehq156
work_keys_str_mv AT takaradashigeho firstevaluationofrealtimenitricoxidechangesinthecoronarycirculationinpatientswithnonischaemicdilatedcardiomyopathyusingacathetertypesensor
AT imanishitoshio firstevaluationofrealtimenitricoxidechangesinthecoronarycirculationinpatientswithnonischaemicdilatedcardiomyopathyusingacathetertypesensor
AT gotomasami firstevaluationofrealtimenitricoxidechangesinthecoronarycirculationinpatientswithnonischaemicdilatedcardiomyopathyusingacathetertypesensor
AT mochizukiseiichi firstevaluationofrealtimenitricoxidechangesinthecoronarycirculationinpatientswithnonischaemicdilatedcardiomyopathyusingacathetertypesensor
AT ikejimahideyuki firstevaluationofrealtimenitricoxidechangesinthecoronarycirculationinpatientswithnonischaemicdilatedcardiomyopathyusingacathetertypesensor
AT tsujiokahiroto firstevaluationofrealtimenitricoxidechangesinthecoronarycirculationinpatientswithnonischaemicdilatedcardiomyopathyusingacathetertypesensor
AT kuroiakio firstevaluationofrealtimenitricoxidechangesinthecoronarycirculationinpatientswithnonischaemicdilatedcardiomyopathyusingacathetertypesensor
AT takeshitatatsuya firstevaluationofrealtimenitricoxidechangesinthecoronarycirculationinpatientswithnonischaemicdilatedcardiomyopathyusingacathetertypesensor
AT akasakatakashi firstevaluationofrealtimenitricoxidechangesinthecoronarycirculationinpatientswithnonischaemicdilatedcardiomyopathyusingacathetertypesensor