Cargando…
Transplanted Oligodendrocytes and Motoneuron Progenitors Generated from Human Embryonic Stem Cells Promote Locomotor Recovery After Spinal Cord Transection
Human embryonic stem cells (hESC) hold great promise for the treatment of patients with many neurodegenerative diseases particularly those arising from cell loss or neural dysfunction including spinal cord injury. This study evaluates the therapeutic effects of transplanted hESC-derived oligodendroc...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Wiley Subscription Services, Inc., A Wiley Company
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2996083/ https://www.ncbi.nlm.nih.gov/pubmed/20665739 http://dx.doi.org/10.1002/stem.489 |
_version_ | 1782193151725797376 |
---|---|
author | Erceg, Slaven Ronaghi, Mohammad Oria, Marc García Roselló, Mireia Aragó, Maria Amparo Pérez Lopez, Maria Gomez Radojevic, Ivana Moreno-Manzano, Victoria Rodríguez-Jiménez, Francisco-Javier Shanker Bhattacharya, Shom Cordoba, Juan Stojkovic, Miodrag |
author_facet | Erceg, Slaven Ronaghi, Mohammad Oria, Marc García Roselló, Mireia Aragó, Maria Amparo Pérez Lopez, Maria Gomez Radojevic, Ivana Moreno-Manzano, Victoria Rodríguez-Jiménez, Francisco-Javier Shanker Bhattacharya, Shom Cordoba, Juan Stojkovic, Miodrag |
author_sort | Erceg, Slaven |
collection | PubMed |
description | Human embryonic stem cells (hESC) hold great promise for the treatment of patients with many neurodegenerative diseases particularly those arising from cell loss or neural dysfunction including spinal cord injury. This study evaluates the therapeutic effects of transplanted hESC-derived oligodendrocyte progenitors (OPC) and/or motoneuron progenitors (MP) on axonal remyelination and functional recovery of adult rats after complete spinal cord transection. OPC and/or MP were grafted into the site of injury in the acute phase. Based on Basso-Beattie-Bresnahan scores recovery of locomotor function was significantly enhanced in rats treated with OPC and/or MP when compared with control animals. When transplanted into the spinal cord immediately after complete transection, OPC and MP survived, migrated, and differentiated into mature oligodendrocytes and neurons showing in vivo electrophysiological activity. Taken together, these results indicate that OPC and MP derived from hESC could be a useful therapeutic strategy to repair injured spinal cord. Stem Cells 2010; 28:1541–1549. |
format | Text |
id | pubmed-2996083 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Wiley Subscription Services, Inc., A Wiley Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-29960832010-12-28 Transplanted Oligodendrocytes and Motoneuron Progenitors Generated from Human Embryonic Stem Cells Promote Locomotor Recovery After Spinal Cord Transection Erceg, Slaven Ronaghi, Mohammad Oria, Marc García Roselló, Mireia Aragó, Maria Amparo Pérez Lopez, Maria Gomez Radojevic, Ivana Moreno-Manzano, Victoria Rodríguez-Jiménez, Francisco-Javier Shanker Bhattacharya, Shom Cordoba, Juan Stojkovic, Miodrag Stem Cells Embryonic Stem Cells/Induced Pluripotent Stem Cells Human embryonic stem cells (hESC) hold great promise for the treatment of patients with many neurodegenerative diseases particularly those arising from cell loss or neural dysfunction including spinal cord injury. This study evaluates the therapeutic effects of transplanted hESC-derived oligodendrocyte progenitors (OPC) and/or motoneuron progenitors (MP) on axonal remyelination and functional recovery of adult rats after complete spinal cord transection. OPC and/or MP were grafted into the site of injury in the acute phase. Based on Basso-Beattie-Bresnahan scores recovery of locomotor function was significantly enhanced in rats treated with OPC and/or MP when compared with control animals. When transplanted into the spinal cord immediately after complete transection, OPC and MP survived, migrated, and differentiated into mature oligodendrocytes and neurons showing in vivo electrophysiological activity. Taken together, these results indicate that OPC and MP derived from hESC could be a useful therapeutic strategy to repair injured spinal cord. Stem Cells 2010; 28:1541–1549. Wiley Subscription Services, Inc., A Wiley Company 2010-09 2010-07-27 /pmc/articles/PMC2996083/ /pubmed/20665739 http://dx.doi.org/10.1002/stem.489 Text en Copyright © 2010 AlphaMed Press http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. |
spellingShingle | Embryonic Stem Cells/Induced Pluripotent Stem Cells Erceg, Slaven Ronaghi, Mohammad Oria, Marc García Roselló, Mireia Aragó, Maria Amparo Pérez Lopez, Maria Gomez Radojevic, Ivana Moreno-Manzano, Victoria Rodríguez-Jiménez, Francisco-Javier Shanker Bhattacharya, Shom Cordoba, Juan Stojkovic, Miodrag Transplanted Oligodendrocytes and Motoneuron Progenitors Generated from Human Embryonic Stem Cells Promote Locomotor Recovery After Spinal Cord Transection |
title | Transplanted Oligodendrocytes and Motoneuron Progenitors Generated from Human Embryonic Stem Cells Promote Locomotor Recovery After Spinal Cord Transection |
title_full | Transplanted Oligodendrocytes and Motoneuron Progenitors Generated from Human Embryonic Stem Cells Promote Locomotor Recovery After Spinal Cord Transection |
title_fullStr | Transplanted Oligodendrocytes and Motoneuron Progenitors Generated from Human Embryonic Stem Cells Promote Locomotor Recovery After Spinal Cord Transection |
title_full_unstemmed | Transplanted Oligodendrocytes and Motoneuron Progenitors Generated from Human Embryonic Stem Cells Promote Locomotor Recovery After Spinal Cord Transection |
title_short | Transplanted Oligodendrocytes and Motoneuron Progenitors Generated from Human Embryonic Stem Cells Promote Locomotor Recovery After Spinal Cord Transection |
title_sort | transplanted oligodendrocytes and motoneuron progenitors generated from human embryonic stem cells promote locomotor recovery after spinal cord transection |
topic | Embryonic Stem Cells/Induced Pluripotent Stem Cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2996083/ https://www.ncbi.nlm.nih.gov/pubmed/20665739 http://dx.doi.org/10.1002/stem.489 |
work_keys_str_mv | AT ercegslaven transplantedoligodendrocytesandmotoneuronprogenitorsgeneratedfromhumanembryonicstemcellspromotelocomotorrecoveryafterspinalcordtransection AT ronaghimohammad transplantedoligodendrocytesandmotoneuronprogenitorsgeneratedfromhumanembryonicstemcellspromotelocomotorrecoveryafterspinalcordtransection AT oriamarc transplantedoligodendrocytesandmotoneuronprogenitorsgeneratedfromhumanembryonicstemcellspromotelocomotorrecoveryafterspinalcordtransection AT garciarosellomireia transplantedoligodendrocytesandmotoneuronprogenitorsgeneratedfromhumanembryonicstemcellspromotelocomotorrecoveryafterspinalcordtransection AT aragomariaamparoperez transplantedoligodendrocytesandmotoneuronprogenitorsgeneratedfromhumanembryonicstemcellspromotelocomotorrecoveryafterspinalcordtransection AT lopezmariagomez transplantedoligodendrocytesandmotoneuronprogenitorsgeneratedfromhumanembryonicstemcellspromotelocomotorrecoveryafterspinalcordtransection AT radojevicivana transplantedoligodendrocytesandmotoneuronprogenitorsgeneratedfromhumanembryonicstemcellspromotelocomotorrecoveryafterspinalcordtransection AT morenomanzanovictoria transplantedoligodendrocytesandmotoneuronprogenitorsgeneratedfromhumanembryonicstemcellspromotelocomotorrecoveryafterspinalcordtransection AT rodriguezjimenezfranciscojavier transplantedoligodendrocytesandmotoneuronprogenitorsgeneratedfromhumanembryonicstemcellspromotelocomotorrecoveryafterspinalcordtransection AT shankerbhattacharyashom transplantedoligodendrocytesandmotoneuronprogenitorsgeneratedfromhumanembryonicstemcellspromotelocomotorrecoveryafterspinalcordtransection AT cordobajuan transplantedoligodendrocytesandmotoneuronprogenitorsgeneratedfromhumanembryonicstemcellspromotelocomotorrecoveryafterspinalcordtransection AT stojkovicmiodrag transplantedoligodendrocytesandmotoneuronprogenitorsgeneratedfromhumanembryonicstemcellspromotelocomotorrecoveryafterspinalcordtransection |