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Recovery of the mitochondrial COI barcode region in diverse Hexapoda through tRNA-based primers

BACKGROUND: DNA barcoding uses a 650 bp segment of the mitochondrial cytochrome c oxidase I (COI) gene as the basis for an identification system for members of the animal kingdom and some other groups of eukaryotes. PCR amplification of the barcode region is a key step in the analytical chain, but i...

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Detalles Bibliográficos
Autores principales: Park, Doo-Sang, Suh, Soo-Jung, Oh, Hyun-Woo, Hebert, Paul DN
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2996951/
https://www.ncbi.nlm.nih.gov/pubmed/20615258
http://dx.doi.org/10.1186/1471-2164-11-423