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Protein Markers for Insulin-Producing Beta Cells with Higher Glucose Sensitivity
BACKGROUND AND METHODOLOGY: Pancreatic beta cells show intercellular differences in their metabolic glucose sensitivity and associated activation of insulin production. To identify protein markers for these variations in functional glucose sensitivity, rat beta cell subpopulations were flow-sorted f...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2997773/ https://www.ncbi.nlm.nih.gov/pubmed/21151894 http://dx.doi.org/10.1371/journal.pone.0014214 |
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author | Martens, Geert A. Jiang, Lei Verhaeghen, Katrijn Connolly, Joanne B. Geromanos, Scott G. Stangé, Geert Van Oudenhove, Laurence Devreese, Bart Hellemans, Karine H. Ling, Zhidong Van Schravendijk, Christiaan Pipeleers, Daniel G. Vissers, Johannes P. C. Gorus, Frans K. |
author_facet | Martens, Geert A. Jiang, Lei Verhaeghen, Katrijn Connolly, Joanne B. Geromanos, Scott G. Stangé, Geert Van Oudenhove, Laurence Devreese, Bart Hellemans, Karine H. Ling, Zhidong Van Schravendijk, Christiaan Pipeleers, Daniel G. Vissers, Johannes P. C. Gorus, Frans K. |
author_sort | Martens, Geert A. |
collection | PubMed |
description | BACKGROUND AND METHODOLOGY: Pancreatic beta cells show intercellular differences in their metabolic glucose sensitivity and associated activation of insulin production. To identify protein markers for these variations in functional glucose sensitivity, rat beta cell subpopulations were flow-sorted for their level of glucose-induced NAD(P)H and their proteomes were quantified by label-free data independent alternate scanning LC-MS. Beta cell-selective proteins were also identified through comparison with rat brain and liver tissue and with purified islet alpha cells, after geometrical normalization using 6 stably expressed reference proteins. PRINCIPAL FINDINGS: All tissues combined, 943 proteins were reliably quantified. In beta cells, 93 out of 467 quantifiable proteins were uniquely detected in this cell type; several other proteins presented a high molar abundance in beta cells. The proteome of the beta cell subpopulation with high metabolic and biosynthetic responsiveness to 7.5 mM glucose was characterized by (i) an on average 50% higher expression of protein biosynthesis regulators such as 40S and 60S ribosomal constituents, NADPH-dependent protein folding factors and translation elongation factors; (ii) 50% higher levels of enzymes involved in glycolysis and in the cytosolic arm of the malate/aspartate-NADH-shuttle. No differences were noticed in mitochondrial enzymes of the Krebs cycle, beta-oxidation or respiratory chain. CONCLUSIONS: Quantification of subtle variations in the proteome using alternate scanning LC-MS shows that beta cell metabolic glucose responsiveness is mostly associated with higher levels of glycolytic but not of mitochondrial enzymes. |
format | Text |
id | pubmed-2997773 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-29977732010-12-10 Protein Markers for Insulin-Producing Beta Cells with Higher Glucose Sensitivity Martens, Geert A. Jiang, Lei Verhaeghen, Katrijn Connolly, Joanne B. Geromanos, Scott G. Stangé, Geert Van Oudenhove, Laurence Devreese, Bart Hellemans, Karine H. Ling, Zhidong Van Schravendijk, Christiaan Pipeleers, Daniel G. Vissers, Johannes P. C. Gorus, Frans K. PLoS One Research Article BACKGROUND AND METHODOLOGY: Pancreatic beta cells show intercellular differences in their metabolic glucose sensitivity and associated activation of insulin production. To identify protein markers for these variations in functional glucose sensitivity, rat beta cell subpopulations were flow-sorted for their level of glucose-induced NAD(P)H and their proteomes were quantified by label-free data independent alternate scanning LC-MS. Beta cell-selective proteins were also identified through comparison with rat brain and liver tissue and with purified islet alpha cells, after geometrical normalization using 6 stably expressed reference proteins. PRINCIPAL FINDINGS: All tissues combined, 943 proteins were reliably quantified. In beta cells, 93 out of 467 quantifiable proteins were uniquely detected in this cell type; several other proteins presented a high molar abundance in beta cells. The proteome of the beta cell subpopulation with high metabolic and biosynthetic responsiveness to 7.5 mM glucose was characterized by (i) an on average 50% higher expression of protein biosynthesis regulators such as 40S and 60S ribosomal constituents, NADPH-dependent protein folding factors and translation elongation factors; (ii) 50% higher levels of enzymes involved in glycolysis and in the cytosolic arm of the malate/aspartate-NADH-shuttle. No differences were noticed in mitochondrial enzymes of the Krebs cycle, beta-oxidation or respiratory chain. CONCLUSIONS: Quantification of subtle variations in the proteome using alternate scanning LC-MS shows that beta cell metabolic glucose responsiveness is mostly associated with higher levels of glycolytic but not of mitochondrial enzymes. Public Library of Science 2010-12-06 /pmc/articles/PMC2997773/ /pubmed/21151894 http://dx.doi.org/10.1371/journal.pone.0014214 Text en Martens et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Martens, Geert A. Jiang, Lei Verhaeghen, Katrijn Connolly, Joanne B. Geromanos, Scott G. Stangé, Geert Van Oudenhove, Laurence Devreese, Bart Hellemans, Karine H. Ling, Zhidong Van Schravendijk, Christiaan Pipeleers, Daniel G. Vissers, Johannes P. C. Gorus, Frans K. Protein Markers for Insulin-Producing Beta Cells with Higher Glucose Sensitivity |
title | Protein Markers for Insulin-Producing Beta Cells with Higher Glucose Sensitivity |
title_full | Protein Markers for Insulin-Producing Beta Cells with Higher Glucose Sensitivity |
title_fullStr | Protein Markers for Insulin-Producing Beta Cells with Higher Glucose Sensitivity |
title_full_unstemmed | Protein Markers for Insulin-Producing Beta Cells with Higher Glucose Sensitivity |
title_short | Protein Markers for Insulin-Producing Beta Cells with Higher Glucose Sensitivity |
title_sort | protein markers for insulin-producing beta cells with higher glucose sensitivity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2997773/ https://www.ncbi.nlm.nih.gov/pubmed/21151894 http://dx.doi.org/10.1371/journal.pone.0014214 |
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