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Artificial Hybrids of Influenza A Virus RNA Polymerase Reveal PA Subunit Modulates Its Thermal Sensitivity

BACKGROUND: Influenza A virus can infect a variety of different hosts and therefore has to adapt to different host temperatures for its efficient viral replication. Influenza virus codes for an RNA polymerase of 3 subunits: PB1, PB2 and PA. It is well known that the PB2 subunit is involved in temper...

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Autores principales: Kashiwagi, Takahito, Hara, Koyu, Nakazono, Yoko, Hamada, Nobuyuki, Watanabe, Hiroshi
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2998429/
https://www.ncbi.nlm.nih.gov/pubmed/21151876
http://dx.doi.org/10.1371/journal.pone.0015140
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author Kashiwagi, Takahito
Hara, Koyu
Nakazono, Yoko
Hamada, Nobuyuki
Watanabe, Hiroshi
author_facet Kashiwagi, Takahito
Hara, Koyu
Nakazono, Yoko
Hamada, Nobuyuki
Watanabe, Hiroshi
author_sort Kashiwagi, Takahito
collection PubMed
description BACKGROUND: Influenza A virus can infect a variety of different hosts and therefore has to adapt to different host temperatures for its efficient viral replication. Influenza virus codes for an RNA polymerase of 3 subunits: PB1, PB2 and PA. It is well known that the PB2 subunit is involved in temperature sensitivity, such as cold adaptation. On the other hand the role of the PA subunit in thermal sensitivity is still poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: To test which polymerase subunit(s) were involved in thermal stress we reconstituted artificial hybrids of influenza RNA polymerase in ribonucleoprotein (RNP) complexes and measured steady-state levels of mRNA, cRNA and vRNA at different temperatures. The PA subunit was involved in modulating RNP activity under thermal stress. Residue 114 of the PA subunit was an important determinant of this activity. CONCLUSIONS/SIGNIFICANCE: These findings suggested that influenza A virus may acquire an RNA polymerase adapted to different body temperatures of the host by reassortment of the RNA polymerase genes.
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spelling pubmed-29984292010-12-10 Artificial Hybrids of Influenza A Virus RNA Polymerase Reveal PA Subunit Modulates Its Thermal Sensitivity Kashiwagi, Takahito Hara, Koyu Nakazono, Yoko Hamada, Nobuyuki Watanabe, Hiroshi PLoS One Research Article BACKGROUND: Influenza A virus can infect a variety of different hosts and therefore has to adapt to different host temperatures for its efficient viral replication. Influenza virus codes for an RNA polymerase of 3 subunits: PB1, PB2 and PA. It is well known that the PB2 subunit is involved in temperature sensitivity, such as cold adaptation. On the other hand the role of the PA subunit in thermal sensitivity is still poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: To test which polymerase subunit(s) were involved in thermal stress we reconstituted artificial hybrids of influenza RNA polymerase in ribonucleoprotein (RNP) complexes and measured steady-state levels of mRNA, cRNA and vRNA at different temperatures. The PA subunit was involved in modulating RNP activity under thermal stress. Residue 114 of the PA subunit was an important determinant of this activity. CONCLUSIONS/SIGNIFICANCE: These findings suggested that influenza A virus may acquire an RNA polymerase adapted to different body temperatures of the host by reassortment of the RNA polymerase genes. Public Library of Science 2010-12-07 /pmc/articles/PMC2998429/ /pubmed/21151876 http://dx.doi.org/10.1371/journal.pone.0015140 Text en Kashiwagi et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kashiwagi, Takahito
Hara, Koyu
Nakazono, Yoko
Hamada, Nobuyuki
Watanabe, Hiroshi
Artificial Hybrids of Influenza A Virus RNA Polymerase Reveal PA Subunit Modulates Its Thermal Sensitivity
title Artificial Hybrids of Influenza A Virus RNA Polymerase Reveal PA Subunit Modulates Its Thermal Sensitivity
title_full Artificial Hybrids of Influenza A Virus RNA Polymerase Reveal PA Subunit Modulates Its Thermal Sensitivity
title_fullStr Artificial Hybrids of Influenza A Virus RNA Polymerase Reveal PA Subunit Modulates Its Thermal Sensitivity
title_full_unstemmed Artificial Hybrids of Influenza A Virus RNA Polymerase Reveal PA Subunit Modulates Its Thermal Sensitivity
title_short Artificial Hybrids of Influenza A Virus RNA Polymerase Reveal PA Subunit Modulates Its Thermal Sensitivity
title_sort artificial hybrids of influenza a virus rna polymerase reveal pa subunit modulates its thermal sensitivity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2998429/
https://www.ncbi.nlm.nih.gov/pubmed/21151876
http://dx.doi.org/10.1371/journal.pone.0015140
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