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A single mutation results in diploid gamete formation and parthenogenesis in a Drosophila yemanuclein-alpha meiosis I defective mutant

BACKGROUND: Sexual reproduction relies on two key events: formation of cells with a haploid genome (the gametes) and restoration of diploidy after fertilization. Therefore the underlying mechanisms must have been evolutionary linked and there is a need for evidence that could support such a model. R...

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Autores principales: Meyer, Régis E, Delaage, Michèle, Rosset, Roland, Capri, Michèle, Aït-Ahmed, Ounissa
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2998452/
https://www.ncbi.nlm.nih.gov/pubmed/21080953
http://dx.doi.org/10.1186/1471-2156-11-104
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author Meyer, Régis E
Delaage, Michèle
Rosset, Roland
Capri, Michèle
Aït-Ahmed, Ounissa
author_facet Meyer, Régis E
Delaage, Michèle
Rosset, Roland
Capri, Michèle
Aït-Ahmed, Ounissa
author_sort Meyer, Régis E
collection PubMed
description BACKGROUND: Sexual reproduction relies on two key events: formation of cells with a haploid genome (the gametes) and restoration of diploidy after fertilization. Therefore the underlying mechanisms must have been evolutionary linked and there is a need for evidence that could support such a model. RESULTS: We describe the identification and the characterization of yem(1), the first yem-alpha mutant allele (V478E), which to some extent affects diploidy reduction and its restoration. Yem-alpha is a member of the Ubinuclein/HPC2 family of proteins that have recently been implicated in playing roles in chromatin remodeling in concert with HIRA histone chaperone. The yem(1 )mutant females exhibited disrupted chromosome behavior in the first meiotic division and produced very low numbers of viable progeny. Unexpectedly these progeny did not display paternal chromosome markers, suggesting that they developed from diploid gametes that underwent gynogenesis, a form of parthenogenesis that requires fertilization. CONCLUSIONS: We focus here on the analysis of the meiotic defects exhibited by yem(1 )oocytes that could account for the formation of diploid gametes. Our results suggest that yem(1 )affects chromosome segregation presumably by affecting kinetochores function in the first meiotic division. This work paves the way to further investigations on the evolution of the mechanisms that support sexual reproduction.
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spelling pubmed-29984522010-12-08 A single mutation results in diploid gamete formation and parthenogenesis in a Drosophila yemanuclein-alpha meiosis I defective mutant Meyer, Régis E Delaage, Michèle Rosset, Roland Capri, Michèle Aït-Ahmed, Ounissa BMC Genet Research Article BACKGROUND: Sexual reproduction relies on two key events: formation of cells with a haploid genome (the gametes) and restoration of diploidy after fertilization. Therefore the underlying mechanisms must have been evolutionary linked and there is a need for evidence that could support such a model. RESULTS: We describe the identification and the characterization of yem(1), the first yem-alpha mutant allele (V478E), which to some extent affects diploidy reduction and its restoration. Yem-alpha is a member of the Ubinuclein/HPC2 family of proteins that have recently been implicated in playing roles in chromatin remodeling in concert with HIRA histone chaperone. The yem(1 )mutant females exhibited disrupted chromosome behavior in the first meiotic division and produced very low numbers of viable progeny. Unexpectedly these progeny did not display paternal chromosome markers, suggesting that they developed from diploid gametes that underwent gynogenesis, a form of parthenogenesis that requires fertilization. CONCLUSIONS: We focus here on the analysis of the meiotic defects exhibited by yem(1 )oocytes that could account for the formation of diploid gametes. Our results suggest that yem(1 )affects chromosome segregation presumably by affecting kinetochores function in the first meiotic division. This work paves the way to further investigations on the evolution of the mechanisms that support sexual reproduction. BioMed Central 2010-11-16 /pmc/articles/PMC2998452/ /pubmed/21080953 http://dx.doi.org/10.1186/1471-2156-11-104 Text en Copyright ©2010 Meyer et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Meyer, Régis E
Delaage, Michèle
Rosset, Roland
Capri, Michèle
Aït-Ahmed, Ounissa
A single mutation results in diploid gamete formation and parthenogenesis in a Drosophila yemanuclein-alpha meiosis I defective mutant
title A single mutation results in diploid gamete formation and parthenogenesis in a Drosophila yemanuclein-alpha meiosis I defective mutant
title_full A single mutation results in diploid gamete formation and parthenogenesis in a Drosophila yemanuclein-alpha meiosis I defective mutant
title_fullStr A single mutation results in diploid gamete formation and parthenogenesis in a Drosophila yemanuclein-alpha meiosis I defective mutant
title_full_unstemmed A single mutation results in diploid gamete formation and parthenogenesis in a Drosophila yemanuclein-alpha meiosis I defective mutant
title_short A single mutation results in diploid gamete formation and parthenogenesis in a Drosophila yemanuclein-alpha meiosis I defective mutant
title_sort single mutation results in diploid gamete formation and parthenogenesis in a drosophila yemanuclein-alpha meiosis i defective mutant
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2998452/
https://www.ncbi.nlm.nih.gov/pubmed/21080953
http://dx.doi.org/10.1186/1471-2156-11-104
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