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Augmented plasma microparticles during acute Plasmodium vivax infection

BACKGROUND: In the last few years, the study of microparticles (MPs) - submicron vesicles released from cells upon activation or apoptosis - has gained growing interest in the field of inflammation and in infectious diseases. Their role in the human malaria parasite Plasmodium vivax remains unexplor...

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Autores principales: Campos, Fernanda MF, Franklin, Bernardo S, Teixeira-Carvalho, Andréa, Filho, Agnaldo LS, de Paula, Sálua CO, Fontes, Cor J, Brito, Cristiana F, Carvalho, Luzia H
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2998527/
https://www.ncbi.nlm.nih.gov/pubmed/21080932
http://dx.doi.org/10.1186/1475-2875-9-327
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author Campos, Fernanda MF
Franklin, Bernardo S
Teixeira-Carvalho, Andréa
Filho, Agnaldo LS
de Paula, Sálua CO
Fontes, Cor J
Brito, Cristiana F
Carvalho, Luzia H
author_facet Campos, Fernanda MF
Franklin, Bernardo S
Teixeira-Carvalho, Andréa
Filho, Agnaldo LS
de Paula, Sálua CO
Fontes, Cor J
Brito, Cristiana F
Carvalho, Luzia H
author_sort Campos, Fernanda MF
collection PubMed
description BACKGROUND: In the last few years, the study of microparticles (MPs) - submicron vesicles released from cells upon activation or apoptosis - has gained growing interest in the field of inflammation and in infectious diseases. Their role in the human malaria parasite Plasmodium vivax remains unexplored. Because acute vivax malaria has been related to pro-inflammatory responses, the main hypothesis investigated in this study was that Plasmodium vivax infection is associated with elevated levels of circulating MPs, which may play a role during acute disease in non-immune patients. METHODS: Plasma MPs were analysed among thirty-seven uncomplicated P. vivax infections from an area of unstable malaria transmission in the Brazilian Amazon. The MP phenotype was analysed by flow cytometry using the classical MP marker, annexin, and fluorochrome-labeled monoclonal antibodies against specific cell surface markers. The frequencies of plasma MPs in P. vivax patients (n = 37) were further compared to malaria-unexposed controls (n = 15) and ovarian carcinoma patients (n = 12), a known MPs-inducing disease non-related to malaria. RESULTS: The frequencies of plasma circulating MPs were markedly increased in P. vivax patients, as compared to healthy age-matched malaria-unexposed controls. Although platelets, erythrocytes and leukocytes were the main cellular sources of MPs during vivax malaria, platelet derived-MPs (PMPs) increased in a linear fashion with the presence of fever at the time of blood collection (β = 0.06, p < 0.0001) and length of acute symptoms (β = 0.36, p < 0.0001). Finally, the results suggest that plasma levels of PMPs diminish as patient experience more episodes of clinical malaria (β = 0.07, p < 0.003). CONCLUSIONS: Abundant circulating MPs are present during acute P. vivax infection, and platelet derived-MPs may play a role on the acute inflammatory symptoms of malaria vivax.
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spelling pubmed-29985272010-12-08 Augmented plasma microparticles during acute Plasmodium vivax infection Campos, Fernanda MF Franklin, Bernardo S Teixeira-Carvalho, Andréa Filho, Agnaldo LS de Paula, Sálua CO Fontes, Cor J Brito, Cristiana F Carvalho, Luzia H Malar J Research BACKGROUND: In the last few years, the study of microparticles (MPs) - submicron vesicles released from cells upon activation or apoptosis - has gained growing interest in the field of inflammation and in infectious diseases. Their role in the human malaria parasite Plasmodium vivax remains unexplored. Because acute vivax malaria has been related to pro-inflammatory responses, the main hypothesis investigated in this study was that Plasmodium vivax infection is associated with elevated levels of circulating MPs, which may play a role during acute disease in non-immune patients. METHODS: Plasma MPs were analysed among thirty-seven uncomplicated P. vivax infections from an area of unstable malaria transmission in the Brazilian Amazon. The MP phenotype was analysed by flow cytometry using the classical MP marker, annexin, and fluorochrome-labeled monoclonal antibodies against specific cell surface markers. The frequencies of plasma MPs in P. vivax patients (n = 37) were further compared to malaria-unexposed controls (n = 15) and ovarian carcinoma patients (n = 12), a known MPs-inducing disease non-related to malaria. RESULTS: The frequencies of plasma circulating MPs were markedly increased in P. vivax patients, as compared to healthy age-matched malaria-unexposed controls. Although platelets, erythrocytes and leukocytes were the main cellular sources of MPs during vivax malaria, platelet derived-MPs (PMPs) increased in a linear fashion with the presence of fever at the time of blood collection (β = 0.06, p < 0.0001) and length of acute symptoms (β = 0.36, p < 0.0001). Finally, the results suggest that plasma levels of PMPs diminish as patient experience more episodes of clinical malaria (β = 0.07, p < 0.003). CONCLUSIONS: Abundant circulating MPs are present during acute P. vivax infection, and platelet derived-MPs may play a role on the acute inflammatory symptoms of malaria vivax. BioMed Central 2010-11-16 /pmc/articles/PMC2998527/ /pubmed/21080932 http://dx.doi.org/10.1186/1475-2875-9-327 Text en Copyright ©2010 Campos et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Campos, Fernanda MF
Franklin, Bernardo S
Teixeira-Carvalho, Andréa
Filho, Agnaldo LS
de Paula, Sálua CO
Fontes, Cor J
Brito, Cristiana F
Carvalho, Luzia H
Augmented plasma microparticles during acute Plasmodium vivax infection
title Augmented plasma microparticles during acute Plasmodium vivax infection
title_full Augmented plasma microparticles during acute Plasmodium vivax infection
title_fullStr Augmented plasma microparticles during acute Plasmodium vivax infection
title_full_unstemmed Augmented plasma microparticles during acute Plasmodium vivax infection
title_short Augmented plasma microparticles during acute Plasmodium vivax infection
title_sort augmented plasma microparticles during acute plasmodium vivax infection
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2998527/
https://www.ncbi.nlm.nih.gov/pubmed/21080932
http://dx.doi.org/10.1186/1475-2875-9-327
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