Determination of Gentamicin Sulphate Composition and Related Substances in Pharmaceutical Preparations by LC with Charged Aerosol Detection

A new, simple and repeatable liquid chromatography method with charged aerosol detection (LC-CAD) for the determination of gentamicin sulphate composition and related substances has been developed. Gentamicin lacks of chromophores, therefore its determination is quite problematic. Using a universal...

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Autores principales: Stypulkowska, Karolina, Blazewicz, Agata, Fijalek, Zbigniew, Sarna, Katarzyna
Formato: Texto
Lenguaje:English
Publicado: Vieweg Verlag 2010
Materias:
Limited Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2998643/
https://www.ncbi.nlm.nih.gov/pubmed/21212825
http://dx.doi.org/10.1365/s10337-010-1763-y
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author Stypulkowska, Karolina
Blazewicz, Agata
Fijalek, Zbigniew
Sarna, Katarzyna
author_facet Stypulkowska, Karolina
Blazewicz, Agata
Fijalek, Zbigniew
Sarna, Katarzyna
author_sort Stypulkowska, Karolina
collection PubMed
description A new, simple and repeatable liquid chromatography method with charged aerosol detection (LC-CAD) for the determination of gentamicin sulphate composition and related substances has been developed. Gentamicin lacks of chromophores, therefore its determination is quite problematic. Using a universal CAD enables to achieve good separation without sample derivatization. Mass spectrometry was employed to confirm the LC-CAD peak profile. The proposed method was validated and applied for the determination of gentamicin sulphate composition and related substances in pharmaceutical preparations.
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spelling pubmed-29986432011-01-04 Determination of Gentamicin Sulphate Composition and Related Substances in Pharmaceutical Preparations by LC with Charged Aerosol Detection Stypulkowska, Karolina Blazewicz, Agata Fijalek, Zbigniew Sarna, Katarzyna Chromatographia Limited Short Communication A new, simple and repeatable liquid chromatography method with charged aerosol detection (LC-CAD) for the determination of gentamicin sulphate composition and related substances has been developed. Gentamicin lacks of chromophores, therefore its determination is quite problematic. Using a universal CAD enables to achieve good separation without sample derivatization. Mass spectrometry was employed to confirm the LC-CAD peak profile. The proposed method was validated and applied for the determination of gentamicin sulphate composition and related substances in pharmaceutical preparations. Vieweg Verlag 2010-10-01 2010 /pmc/articles/PMC2998643/ /pubmed/21212825 http://dx.doi.org/10.1365/s10337-010-1763-y Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Limited Short Communication
Stypulkowska, Karolina
Blazewicz, Agata
Fijalek, Zbigniew
Sarna, Katarzyna
Determination of Gentamicin Sulphate Composition and Related Substances in Pharmaceutical Preparations by LC with Charged Aerosol Detection
title Determination of Gentamicin Sulphate Composition and Related Substances in Pharmaceutical Preparations by LC with Charged Aerosol Detection
title_full Determination of Gentamicin Sulphate Composition and Related Substances in Pharmaceutical Preparations by LC with Charged Aerosol Detection
title_fullStr Determination of Gentamicin Sulphate Composition and Related Substances in Pharmaceutical Preparations by LC with Charged Aerosol Detection
title_full_unstemmed Determination of Gentamicin Sulphate Composition and Related Substances in Pharmaceutical Preparations by LC with Charged Aerosol Detection
title_short Determination of Gentamicin Sulphate Composition and Related Substances in Pharmaceutical Preparations by LC with Charged Aerosol Detection
title_sort determination of gentamicin sulphate composition and related substances in pharmaceutical preparations by lc with charged aerosol detection
topic Limited Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2998643/
https://www.ncbi.nlm.nih.gov/pubmed/21212825
http://dx.doi.org/10.1365/s10337-010-1763-y
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