Development of a universal plate-agglutination test for detecting Haemophilus parasuis

Due to the serovar diversity in Haemophilus (H.) parasuis, it is difficult to develop a universal serological method for detection of this pathogen. Here, we report a universal plate-agglutination test for detecting H. parasuis. Diagnostic antisera were prepared by mixing antisera of serovars 4, 5,...

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Detalles Bibliográficos
Autores principales: Guo, Dingqian, Tang, Cheng, Hai, Quan, Shao, Guoqing, Yue, Hua
Formato: Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2010
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2998749/
https://www.ncbi.nlm.nih.gov/pubmed/21113107
http://dx.doi.org/10.4142/jvs.2010.11.4.355
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author Guo, Dingqian
Tang, Cheng
Hai, Quan
Shao, Guoqing
Yue, Hua
author_facet Guo, Dingqian
Tang, Cheng
Hai, Quan
Shao, Guoqing
Yue, Hua
author_sort Guo, Dingqian
collection PubMed
description Due to the serovar diversity in Haemophilus (H.) parasuis, it is difficult to develop a universal serological method for detection of this pathogen. Here, we report a universal plate-agglutination test for detecting H. parasuis. Diagnostic antisera were prepared by mixing antisera of serovars 4, 5, 12, 13 and 14 in the optimized ratio. The results of the plate-agglutination test showed that the diagnostic antisera could agglutinate with all 15 reference strains of H. parasuis and 74/75 clinical isolates. Further, the specificity of the method was validated with 22 bacterial strains from 12 related species.
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spelling pubmed-29987492010-12-22 Development of a universal plate-agglutination test for detecting Haemophilus parasuis Guo, Dingqian Tang, Cheng Hai, Quan Shao, Guoqing Yue, Hua J Vet Sci Short Communication Due to the serovar diversity in Haemophilus (H.) parasuis, it is difficult to develop a universal serological method for detection of this pathogen. Here, we report a universal plate-agglutination test for detecting H. parasuis. Diagnostic antisera were prepared by mixing antisera of serovars 4, 5, 12, 13 and 14 in the optimized ratio. The results of the plate-agglutination test showed that the diagnostic antisera could agglutinate with all 15 reference strains of H. parasuis and 74/75 clinical isolates. Further, the specificity of the method was validated with 22 bacterial strains from 12 related species. The Korean Society of Veterinary Science 2010-12 2010-12-03 /pmc/articles/PMC2998749/ /pubmed/21113107 http://dx.doi.org/10.4142/jvs.2010.11.4.355 Text en Copyright © 2010 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Guo, Dingqian
Tang, Cheng
Hai, Quan
Shao, Guoqing
Yue, Hua
Development of a universal plate-agglutination test for detecting Haemophilus parasuis
title Development of a universal plate-agglutination test for detecting Haemophilus parasuis
title_full Development of a universal plate-agglutination test for detecting Haemophilus parasuis
title_fullStr Development of a universal plate-agglutination test for detecting Haemophilus parasuis
title_full_unstemmed Development of a universal plate-agglutination test for detecting Haemophilus parasuis
title_short Development of a universal plate-agglutination test for detecting Haemophilus parasuis
title_sort development of a universal plate-agglutination test for detecting haemophilus parasuis
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2998749/
https://www.ncbi.nlm.nih.gov/pubmed/21113107
http://dx.doi.org/10.4142/jvs.2010.11.4.355
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