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Characterization of Microsatellite Loci in the Western Subterranean Termite, Reticulitermes hesperus, and Cross-Amplification in Closely Related Cryptic Species

New, and previously reported microsatellites, were characterized for a group of four cryptic sibling species in California (USA) in the subterranean termite genus Reticulitermes with the goal of finding loci appropriate for population and species level studies. Three new microsatellites were identif...

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Autor principal: Copren, Kirsten A.
Formato: Texto
Lenguaje:English
Publicado: University of Wisconsin Library 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2999412/
https://www.ncbi.nlm.nih.gov/pubmed/20307236
http://dx.doi.org/10.1673/031.007.1701
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author Copren, Kirsten A.
author_facet Copren, Kirsten A.
author_sort Copren, Kirsten A.
collection PubMed
description New, and previously reported microsatellites, were characterized for a group of four cryptic sibling species in California (USA) in the subterranean termite genus Reticulitermes with the goal of finding loci appropriate for population and species level studies. Three new microsatellites were identified originating from R. hesperus, and 19 loci previously characterized in R. flavipes and R. santonensis were examined. Of the three loci specifically developed for R. hesperus, none amplify with the other species. Variation appropriate for population level studies was found in 4–13 loci depending on the species. Fifteen loci appeared to be appropriate for use at the species level. Unique or monomorphic alleles are found among the four species, indicating these loci will be taxonomically informative for this group.
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spelling pubmed-29994122010-12-09 Characterization of Microsatellite Loci in the Western Subterranean Termite, Reticulitermes hesperus, and Cross-Amplification in Closely Related Cryptic Species Copren, Kirsten A. J Insect Sci Article New, and previously reported microsatellites, were characterized for a group of four cryptic sibling species in California (USA) in the subterranean termite genus Reticulitermes with the goal of finding loci appropriate for population and species level studies. Three new microsatellites were identified originating from R. hesperus, and 19 loci previously characterized in R. flavipes and R. santonensis were examined. Of the three loci specifically developed for R. hesperus, none amplify with the other species. Variation appropriate for population level studies was found in 4–13 loci depending on the species. Fifteen loci appeared to be appropriate for use at the species level. Unique or monomorphic alleles are found among the four species, indicating these loci will be taxonomically informative for this group. University of Wisconsin Library 2007-03-29 /pmc/articles/PMC2999412/ /pubmed/20307236 http://dx.doi.org/10.1673/031.007.1701 Text en © 2007 http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Copren, Kirsten A.
Characterization of Microsatellite Loci in the Western Subterranean Termite, Reticulitermes hesperus, and Cross-Amplification in Closely Related Cryptic Species
title Characterization of Microsatellite Loci in the Western Subterranean Termite, Reticulitermes hesperus, and Cross-Amplification in Closely Related Cryptic Species
title_full Characterization of Microsatellite Loci in the Western Subterranean Termite, Reticulitermes hesperus, and Cross-Amplification in Closely Related Cryptic Species
title_fullStr Characterization of Microsatellite Loci in the Western Subterranean Termite, Reticulitermes hesperus, and Cross-Amplification in Closely Related Cryptic Species
title_full_unstemmed Characterization of Microsatellite Loci in the Western Subterranean Termite, Reticulitermes hesperus, and Cross-Amplification in Closely Related Cryptic Species
title_short Characterization of Microsatellite Loci in the Western Subterranean Termite, Reticulitermes hesperus, and Cross-Amplification in Closely Related Cryptic Species
title_sort characterization of microsatellite loci in the western subterranean termite, reticulitermes hesperus, and cross-amplification in closely related cryptic species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2999412/
https://www.ncbi.nlm.nih.gov/pubmed/20307236
http://dx.doi.org/10.1673/031.007.1701
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