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Characterization of Microsatellite Loci in the Western Subterranean Termite, Reticulitermes hesperus, and Cross-Amplification in Closely Related Cryptic Species
New, and previously reported microsatellites, were characterized for a group of four cryptic sibling species in California (USA) in the subterranean termite genus Reticulitermes with the goal of finding loci appropriate for population and species level studies. Three new microsatellites were identif...
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Formato: | Texto |
Lenguaje: | English |
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University of Wisconsin Library
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2999412/ https://www.ncbi.nlm.nih.gov/pubmed/20307236 http://dx.doi.org/10.1673/031.007.1701 |
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author | Copren, Kirsten A. |
author_facet | Copren, Kirsten A. |
author_sort | Copren, Kirsten A. |
collection | PubMed |
description | New, and previously reported microsatellites, were characterized for a group of four cryptic sibling species in California (USA) in the subterranean termite genus Reticulitermes with the goal of finding loci appropriate for population and species level studies. Three new microsatellites were identified originating from R. hesperus, and 19 loci previously characterized in R. flavipes and R. santonensis were examined. Of the three loci specifically developed for R. hesperus, none amplify with the other species. Variation appropriate for population level studies was found in 4–13 loci depending on the species. Fifteen loci appeared to be appropriate for use at the species level. Unique or monomorphic alleles are found among the four species, indicating these loci will be taxonomically informative for this group. |
format | Text |
id | pubmed-2999412 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | University of Wisconsin Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-29994122010-12-09 Characterization of Microsatellite Loci in the Western Subterranean Termite, Reticulitermes hesperus, and Cross-Amplification in Closely Related Cryptic Species Copren, Kirsten A. J Insect Sci Article New, and previously reported microsatellites, were characterized for a group of four cryptic sibling species in California (USA) in the subterranean termite genus Reticulitermes with the goal of finding loci appropriate for population and species level studies. Three new microsatellites were identified originating from R. hesperus, and 19 loci previously characterized in R. flavipes and R. santonensis were examined. Of the three loci specifically developed for R. hesperus, none amplify with the other species. Variation appropriate for population level studies was found in 4–13 loci depending on the species. Fifteen loci appeared to be appropriate for use at the species level. Unique or monomorphic alleles are found among the four species, indicating these loci will be taxonomically informative for this group. University of Wisconsin Library 2007-03-29 /pmc/articles/PMC2999412/ /pubmed/20307236 http://dx.doi.org/10.1673/031.007.1701 Text en © 2007 http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Copren, Kirsten A. Characterization of Microsatellite Loci in the Western Subterranean Termite, Reticulitermes hesperus, and Cross-Amplification in Closely Related Cryptic Species |
title | Characterization of Microsatellite Loci in the Western Subterranean Termite, Reticulitermes hesperus, and Cross-Amplification in Closely Related Cryptic Species |
title_full | Characterization of Microsatellite Loci in the Western Subterranean Termite, Reticulitermes hesperus, and Cross-Amplification in Closely Related Cryptic Species |
title_fullStr | Characterization of Microsatellite Loci in the Western Subterranean Termite, Reticulitermes hesperus, and Cross-Amplification in Closely Related Cryptic Species |
title_full_unstemmed | Characterization of Microsatellite Loci in the Western Subterranean Termite, Reticulitermes hesperus, and Cross-Amplification in Closely Related Cryptic Species |
title_short | Characterization of Microsatellite Loci in the Western Subterranean Termite, Reticulitermes hesperus, and Cross-Amplification in Closely Related Cryptic Species |
title_sort | characterization of microsatellite loci in the western subterranean termite, reticulitermes hesperus, and cross-amplification in closely related cryptic species |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2999412/ https://www.ncbi.nlm.nih.gov/pubmed/20307236 http://dx.doi.org/10.1673/031.007.1701 |
work_keys_str_mv | AT coprenkirstena characterizationofmicrosatellitelociinthewesternsubterraneantermitereticulitermeshesperusandcrossamplificationincloselyrelatedcrypticspecies |