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Copper Transporter 2 Content Is Lower in Liver and Heart of Copper-Deficient Rats

Copper (Cu) transporter 2 (Ctr2) is a transmembrane protein that transports Cu across cell membranes and increases cytosolic Cu levels. Experiments using cell lines have suggested that Ctr2 expression is regulated by Cu status. The importance of changes in Ctr2 expression is underscored by recent st...

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Autores principales: Bertinato, Jesse, Duval, Sébastien, L’Abbé, Mary R.
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3000112/
https://www.ncbi.nlm.nih.gov/pubmed/21151468
http://dx.doi.org/10.3390/ijms11114741
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author Bertinato, Jesse
Duval, Sébastien
L’Abbé, Mary R.
author_facet Bertinato, Jesse
Duval, Sébastien
L’Abbé, Mary R.
author_sort Bertinato, Jesse
collection PubMed
description Copper (Cu) transporter 2 (Ctr2) is a transmembrane protein that transports Cu across cell membranes and increases cytosolic Cu levels. Experiments using cell lines have suggested that Ctr2 expression is regulated by Cu status. The importance of changes in Ctr2 expression is underscored by recent studies demonstrating that lower Ctr2 content in cells increases the cellular uptake of platinum-containing cancer drugs and toxicity to the drugs. In this study, we examined whether Ctr2 expression is altered by a nutritional Cu deficiency in vivo. Ctr2 mRNA and protein in liver and heart from rats fed a normal (Cu-N), moderately deficient (Cu-M) or deficient (Cu-D) Cu diet was measured. Rats fed the Cu-deficient diets showed a dose-dependent decrease in liver Ctr2 protein compared to Cu-N rats. Ctr2 protein was 42% and 85% lower in Cu-M and Cu-D rats, respectively. Liver Ctr2 mRNA was 50% lower in Cu-D rats and unaffected in Cu-M rats. In heart, Ctr2 protein was only lower in Cu-D rats (46% lower). These data show that Cu deficiency decreases Ctr2 content in vivo.
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spelling pubmed-30001122010-12-10 Copper Transporter 2 Content Is Lower in Liver and Heart of Copper-Deficient Rats Bertinato, Jesse Duval, Sébastien L’Abbé, Mary R. Int J Mol Sci Brief Report Copper (Cu) transporter 2 (Ctr2) is a transmembrane protein that transports Cu across cell membranes and increases cytosolic Cu levels. Experiments using cell lines have suggested that Ctr2 expression is regulated by Cu status. The importance of changes in Ctr2 expression is underscored by recent studies demonstrating that lower Ctr2 content in cells increases the cellular uptake of platinum-containing cancer drugs and toxicity to the drugs. In this study, we examined whether Ctr2 expression is altered by a nutritional Cu deficiency in vivo. Ctr2 mRNA and protein in liver and heart from rats fed a normal (Cu-N), moderately deficient (Cu-M) or deficient (Cu-D) Cu diet was measured. Rats fed the Cu-deficient diets showed a dose-dependent decrease in liver Ctr2 protein compared to Cu-N rats. Ctr2 protein was 42% and 85% lower in Cu-M and Cu-D rats, respectively. Liver Ctr2 mRNA was 50% lower in Cu-D rats and unaffected in Cu-M rats. In heart, Ctr2 protein was only lower in Cu-D rats (46% lower). These data show that Cu deficiency decreases Ctr2 content in vivo. Molecular Diversity Preservation International (MDPI) 2010-11-19 /pmc/articles/PMC3000112/ /pubmed/21151468 http://dx.doi.org/10.3390/ijms11114741 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Brief Report
Bertinato, Jesse
Duval, Sébastien
L’Abbé, Mary R.
Copper Transporter 2 Content Is Lower in Liver and Heart of Copper-Deficient Rats
title Copper Transporter 2 Content Is Lower in Liver and Heart of Copper-Deficient Rats
title_full Copper Transporter 2 Content Is Lower in Liver and Heart of Copper-Deficient Rats
title_fullStr Copper Transporter 2 Content Is Lower in Liver and Heart of Copper-Deficient Rats
title_full_unstemmed Copper Transporter 2 Content Is Lower in Liver and Heart of Copper-Deficient Rats
title_short Copper Transporter 2 Content Is Lower in Liver and Heart of Copper-Deficient Rats
title_sort copper transporter 2 content is lower in liver and heart of copper-deficient rats
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3000112/
https://www.ncbi.nlm.nih.gov/pubmed/21151468
http://dx.doi.org/10.3390/ijms11114741
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