Cellular Reactive Oxygen Species Inhibit MPYS Induction of IFNβ

Many inflammatory diseases, as well as infections, are accompanied by elevation in cellular levels of Reactive Oxygen Species (ROS). Here we report that MPYS, a.k.a. STING, which was recently shown to mediate activation of IFNβ expression during infection, is a ROS sensor. ROS induce intermolecular...

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Detalles Bibliográficos
Autores principales: Jin, Lei, Lenz, Laurel L., Cambier, John C.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3000824/
https://www.ncbi.nlm.nih.gov/pubmed/21170271
http://dx.doi.org/10.1371/journal.pone.0015142
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author Jin, Lei
Lenz, Laurel L.
Cambier, John C.
author_facet Jin, Lei
Lenz, Laurel L.
Cambier, John C.
author_sort Jin, Lei
collection PubMed
description Many inflammatory diseases, as well as infections, are accompanied by elevation in cellular levels of Reactive Oxygen Species (ROS). Here we report that MPYS, a.k.a. STING, which was recently shown to mediate activation of IFNβ expression during infection, is a ROS sensor. ROS induce intermolecular disulfide bonds formation in MPYS homodimer and inhibit MPYS IFNβ stimulatory activity. Cys-64, -148, -292, -309 and the potential C(88)xxC(91) redox motif in MPYS are indispensable for IFNβ stimulation and IRF3 activation. Thus, our results identify a novel mechanism for ROS regulation of IFNβ stimulation.
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spelling pubmed-30008242010-12-17 Cellular Reactive Oxygen Species Inhibit MPYS Induction of IFNβ Jin, Lei Lenz, Laurel L. Cambier, John C. PLoS One Research Article Many inflammatory diseases, as well as infections, are accompanied by elevation in cellular levels of Reactive Oxygen Species (ROS). Here we report that MPYS, a.k.a. STING, which was recently shown to mediate activation of IFNβ expression during infection, is a ROS sensor. ROS induce intermolecular disulfide bonds formation in MPYS homodimer and inhibit MPYS IFNβ stimulatory activity. Cys-64, -148, -292, -309 and the potential C(88)xxC(91) redox motif in MPYS are indispensable for IFNβ stimulation and IRF3 activation. Thus, our results identify a novel mechanism for ROS regulation of IFNβ stimulation. Public Library of Science 2010-12-10 /pmc/articles/PMC3000824/ /pubmed/21170271 http://dx.doi.org/10.1371/journal.pone.0015142 Text en Jin et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jin, Lei
Lenz, Laurel L.
Cambier, John C.
Cellular Reactive Oxygen Species Inhibit MPYS Induction of IFNβ
title Cellular Reactive Oxygen Species Inhibit MPYS Induction of IFNβ
title_full Cellular Reactive Oxygen Species Inhibit MPYS Induction of IFNβ
title_fullStr Cellular Reactive Oxygen Species Inhibit MPYS Induction of IFNβ
title_full_unstemmed Cellular Reactive Oxygen Species Inhibit MPYS Induction of IFNβ
title_short Cellular Reactive Oxygen Species Inhibit MPYS Induction of IFNβ
title_sort cellular reactive oxygen species inhibit mpys induction of ifnβ
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3000824/
https://www.ncbi.nlm.nih.gov/pubmed/21170271
http://dx.doi.org/10.1371/journal.pone.0015142
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AT lenzlaurell cellularreactiveoxygenspeciesinhibitmpysinductionofifnb
AT cambierjohnc cellularreactiveoxygenspeciesinhibitmpysinductionofifnb

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