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Dissecting the role of conserved box C/D sRNA sequences in di-sRNP assembly and function

In all three kingdoms of life, nucleotides in ribosomal RNA (rRNA) are post-transcriptionally modified. One type of chemical modification is 2′-O-ribose methylation, which is, in eukaryotes and archaea, performed by box C/D small ribonucleoproteins (box C/D sRNPs in archaea) and box C/D small nucleo...

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Detalles Bibliográficos
Autores principales: Bleichert, Franziska, Baserga, Susan J.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3001065/
https://www.ncbi.nlm.nih.gov/pubmed/20693534
http://dx.doi.org/10.1093/nar/gkq690
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author Bleichert, Franziska
Baserga, Susan J.
author_facet Bleichert, Franziska
Baserga, Susan J.
author_sort Bleichert, Franziska
collection PubMed
description In all three kingdoms of life, nucleotides in ribosomal RNA (rRNA) are post-transcriptionally modified. One type of chemical modification is 2′-O-ribose methylation, which is, in eukaryotes and archaea, performed by box C/D small ribonucleoproteins (box C/D sRNPs in archaea) and box C/D small nucleolar ribonucleoproteins (box C/D snoRNPs in eukaryotes), respectively. Recently, the first structure of any catalytically active box C/D s(no)RNP determined by electron microscopy and single particle analysis surprisingly demonstrated that they are dimeric RNPs. Mutational analyses of the Nop5 protein interface suggested that di-sRNP formation is also required for the in vitro catalytic activity. We have now analyzed the functional relevance of the second interface, the sRNA interface, within the box C/D di-sRNP. Mutations in conserved sequence elements of the sRNA, which allow sRNP assembly but which severely interfere with the catalytic activity of box C/D sRNPs, prevent formation of the di-sRNP. In addition, we can observe the dimeric box C/D sRNP architecture with a different box C/D sRNP, suggesting that this architecture is conserved. Together, these results provide further support for the functional relevance of the di-sRNP architecture and also provide a structural explanation for the observed defects in catalysis of 2′-O-ribose methylation.
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spelling pubmed-30010652010-12-13 Dissecting the role of conserved box C/D sRNA sequences in di-sRNP assembly and function Bleichert, Franziska Baserga, Susan J. Nucleic Acids Res RNA In all three kingdoms of life, nucleotides in ribosomal RNA (rRNA) are post-transcriptionally modified. One type of chemical modification is 2′-O-ribose methylation, which is, in eukaryotes and archaea, performed by box C/D small ribonucleoproteins (box C/D sRNPs in archaea) and box C/D small nucleolar ribonucleoproteins (box C/D snoRNPs in eukaryotes), respectively. Recently, the first structure of any catalytically active box C/D s(no)RNP determined by electron microscopy and single particle analysis surprisingly demonstrated that they are dimeric RNPs. Mutational analyses of the Nop5 protein interface suggested that di-sRNP formation is also required for the in vitro catalytic activity. We have now analyzed the functional relevance of the second interface, the sRNA interface, within the box C/D di-sRNP. Mutations in conserved sequence elements of the sRNA, which allow sRNP assembly but which severely interfere with the catalytic activity of box C/D sRNPs, prevent formation of the di-sRNP. In addition, we can observe the dimeric box C/D sRNP architecture with a different box C/D sRNP, suggesting that this architecture is conserved. Together, these results provide further support for the functional relevance of the di-sRNP architecture and also provide a structural explanation for the observed defects in catalysis of 2′-O-ribose methylation. Oxford University Press 2010-12 2010-08-06 /pmc/articles/PMC3001065/ /pubmed/20693534 http://dx.doi.org/10.1093/nar/gkq690 Text en © The Author(s) 2010. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA
Bleichert, Franziska
Baserga, Susan J.
Dissecting the role of conserved box C/D sRNA sequences in di-sRNP assembly and function
title Dissecting the role of conserved box C/D sRNA sequences in di-sRNP assembly and function
title_full Dissecting the role of conserved box C/D sRNA sequences in di-sRNP assembly and function
title_fullStr Dissecting the role of conserved box C/D sRNA sequences in di-sRNP assembly and function
title_full_unstemmed Dissecting the role of conserved box C/D sRNA sequences in di-sRNP assembly and function
title_short Dissecting the role of conserved box C/D sRNA sequences in di-sRNP assembly and function
title_sort dissecting the role of conserved box c/d srna sequences in di-srnp assembly and function
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3001065/
https://www.ncbi.nlm.nih.gov/pubmed/20693534
http://dx.doi.org/10.1093/nar/gkq690
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