Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression

Mouse transgenesis has proven invaluable for analysis of gene function and generation of human disease models. We describe here the development of a pronuclear injection-based targeted transgenesis (PITT) system, involving site-specific integration in fertilized eggs. The system was applied to two d...

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Detalles Bibliográficos
Autores principales: Ohtsuka, Masato, Ogiwara, Sanae, Miura, Hiromi, Mizutani, Akiko, Warita, Takayuki, Sato, Masahiro, Imai, Kenji, Hozumi, Katsuto, Sato, Takehito, Tanaka, Masafumi, Kimura, Minoru, Inoko, Hidetoshi
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3001095/
https://www.ncbi.nlm.nih.gov/pubmed/20880997
http://dx.doi.org/10.1093/nar/gkq860

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3001095/
https://www.ncbi.nlm.nih.gov/pubmed/20880997
http://dx.doi.org/10.1093/nar/gkq860

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