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Purification and In Situ Immobilization of Papain with Aqueous Two-Phase System

Papain was purified from spray-dried Carica papaya latex using aqueous two-phase system (ATPS). Then it was recovered from PEG phase by in situ immobilization or preparing cross-linked enzyme aggregates (CLEAs). The Plackett-Burman design and the central composite design (CCD) together with the resp...

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Detalles Bibliográficos
Autores principales:	Li, Mingliang, Su, Erzheng, You, Pengyong, Gong, Xiangyu, Sun, Ming, Xu, Diansheng, Wei, Dongzhi
Formato:	Texto
Lenguaje:	English
Publicado:	Public Library of Science 2010
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3001450/ https://www.ncbi.nlm.nih.gov/pubmed/21179198 http://dx.doi.org/10.1371/journal.pone.0015168

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