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Comprehensive Gene-Expression Survey Identifies Wif1 as a Modulator of Cardiomyocyte Differentiation
During chicken cardiac development the proepicardium (PE) forms the epicardium (Epi), which contributes to several non-myocardial lineages within the heart. In contrast to Epi-explant cultures, PE explants can differentiate into a cardiomyocyte phenotype. By temporal microarray expression profiles o...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3001492/ https://www.ncbi.nlm.nih.gov/pubmed/21179454 http://dx.doi.org/10.1371/journal.pone.0015504 |
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author | Buermans, Henk P. J. van Wijk, Bram Hulsker, Margriet A. Smit, Niels C. H. den Dunnen, Johan T. van Ommen, Gertjan B. Moorman, Antoon F. van den Hoff, Maurice J. 't Hoen, Peter A. C. |
author_facet | Buermans, Henk P. J. van Wijk, Bram Hulsker, Margriet A. Smit, Niels C. H. den Dunnen, Johan T. van Ommen, Gertjan B. Moorman, Antoon F. van den Hoff, Maurice J. 't Hoen, Peter A. C. |
author_sort | Buermans, Henk P. J. |
collection | PubMed |
description | During chicken cardiac development the proepicardium (PE) forms the epicardium (Epi), which contributes to several non-myocardial lineages within the heart. In contrast to Epi-explant cultures, PE explants can differentiate into a cardiomyocyte phenotype. By temporal microarray expression profiles of PE-explant cultures and maturing Epi cells, we identified genes specifically associated with differentiation towards either of these lineages and genes that are associated with the Epi-lineage restriction. We found a central role for Wnt signaling in the determination of the different cell lineages. Immunofluorescent staining after recombinant-protein incubation in PE-explant cultures indicated that the early upregulated Wnt inhibitory factor-1 (Wif1), stimulates cardiomyocyte differentiation in a similar manner as Wnt stimulation. Concordingly, in the mouse pluripotent embryogenic carcinoma cell line p19cl6, early and late Wif1 exposure enhances and attenuates differentiation, respectively. In ovo exposure of the HH12 chicken embryonic heart to Wif1 increases the Tbx18-positive cardiac progenitor pool. These data indicate that Wif1 enhances cardiomyogenesis. |
format | Text |
id | pubmed-3001492 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30014922010-12-21 Comprehensive Gene-Expression Survey Identifies Wif1 as a Modulator of Cardiomyocyte Differentiation Buermans, Henk P. J. van Wijk, Bram Hulsker, Margriet A. Smit, Niels C. H. den Dunnen, Johan T. van Ommen, Gertjan B. Moorman, Antoon F. van den Hoff, Maurice J. 't Hoen, Peter A. C. PLoS One Research Article During chicken cardiac development the proepicardium (PE) forms the epicardium (Epi), which contributes to several non-myocardial lineages within the heart. In contrast to Epi-explant cultures, PE explants can differentiate into a cardiomyocyte phenotype. By temporal microarray expression profiles of PE-explant cultures and maturing Epi cells, we identified genes specifically associated with differentiation towards either of these lineages and genes that are associated with the Epi-lineage restriction. We found a central role for Wnt signaling in the determination of the different cell lineages. Immunofluorescent staining after recombinant-protein incubation in PE-explant cultures indicated that the early upregulated Wnt inhibitory factor-1 (Wif1), stimulates cardiomyocyte differentiation in a similar manner as Wnt stimulation. Concordingly, in the mouse pluripotent embryogenic carcinoma cell line p19cl6, early and late Wif1 exposure enhances and attenuates differentiation, respectively. In ovo exposure of the HH12 chicken embryonic heart to Wif1 increases the Tbx18-positive cardiac progenitor pool. These data indicate that Wif1 enhances cardiomyogenesis. Public Library of Science 2010-12-13 /pmc/articles/PMC3001492/ /pubmed/21179454 http://dx.doi.org/10.1371/journal.pone.0015504 Text en Buermans et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Buermans, Henk P. J. van Wijk, Bram Hulsker, Margriet A. Smit, Niels C. H. den Dunnen, Johan T. van Ommen, Gertjan B. Moorman, Antoon F. van den Hoff, Maurice J. 't Hoen, Peter A. C. Comprehensive Gene-Expression Survey Identifies Wif1 as a Modulator of Cardiomyocyte Differentiation |
title | Comprehensive Gene-Expression Survey Identifies Wif1 as a Modulator of Cardiomyocyte Differentiation |
title_full | Comprehensive Gene-Expression Survey Identifies Wif1 as a Modulator of Cardiomyocyte Differentiation |
title_fullStr | Comprehensive Gene-Expression Survey Identifies Wif1 as a Modulator of Cardiomyocyte Differentiation |
title_full_unstemmed | Comprehensive Gene-Expression Survey Identifies Wif1 as a Modulator of Cardiomyocyte Differentiation |
title_short | Comprehensive Gene-Expression Survey Identifies Wif1 as a Modulator of Cardiomyocyte Differentiation |
title_sort | comprehensive gene-expression survey identifies wif1 as a modulator of cardiomyocyte differentiation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3001492/ https://www.ncbi.nlm.nih.gov/pubmed/21179454 http://dx.doi.org/10.1371/journal.pone.0015504 |
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