Cargando…

CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex

CDK5RAP2 is a human microcephaly protein that contains a γ-tubulin complex (γ-TuC)–binding domain conserved in Drosophila melanogaster centrosomin and Schizosaccharomyces pombe Mto1p and Pcp1p, which are γ-TuC–tethering proteins. In this study, we show that this domain within CDK5RAP2 associates wit...

Descripción completa

Detalles Bibliográficos
Autores principales: Choi, Yuk-Kwan, Liu, Pengfei, Sze, Siu Kwan, Dai, Chao, Qi, Robert Z.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002024/
https://www.ncbi.nlm.nih.gov/pubmed/21135143
http://dx.doi.org/10.1083/jcb.201007030
_version_ 1782193686305570816
author Choi, Yuk-Kwan
Liu, Pengfei
Sze, Siu Kwan
Dai, Chao
Qi, Robert Z.
author_facet Choi, Yuk-Kwan
Liu, Pengfei
Sze, Siu Kwan
Dai, Chao
Qi, Robert Z.
author_sort Choi, Yuk-Kwan
collection PubMed
description CDK5RAP2 is a human microcephaly protein that contains a γ-tubulin complex (γ-TuC)–binding domain conserved in Drosophila melanogaster centrosomin and Schizosaccharomyces pombe Mto1p and Pcp1p, which are γ-TuC–tethering proteins. In this study, we show that this domain within CDK5RAP2 associates with the γ-tubulin ring complex (γ-TuRC) to stimulate its microtubule-nucleating activity and is therefore referred to as the γ-TuRC–mediated nucleation activator (γ-TuNA). γ-TuNA but not its γ-TuC–binding-deficient mutant stimulates microtubule nucleation by purified γ-TuRC in vitro and induces extensive, γ-TuRC-dependent nucleation of microtubules in a microtubule regrowth assay. γ-TuRC bound to γ-TuNA contains NME7, FAM128A/B, and actin in addition to γ-tubulin and GCP2–6. RNA interference–mediated depletion of CDK5RAP2 impairs both centrosomal and acentrosomal microtubule nucleation, although γ-TuRC assembly is unaffected. Collectively, these results suggest that the γ-TuNA found in CDK5RAP2 has regulatory functions in γ-TuRC–mediated microtubule nucleation.
format Text
id pubmed-3002024
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-30020242011-06-13 CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex Choi, Yuk-Kwan Liu, Pengfei Sze, Siu Kwan Dai, Chao Qi, Robert Z. J Cell Biol Research Articles CDK5RAP2 is a human microcephaly protein that contains a γ-tubulin complex (γ-TuC)–binding domain conserved in Drosophila melanogaster centrosomin and Schizosaccharomyces pombe Mto1p and Pcp1p, which are γ-TuC–tethering proteins. In this study, we show that this domain within CDK5RAP2 associates with the γ-tubulin ring complex (γ-TuRC) to stimulate its microtubule-nucleating activity and is therefore referred to as the γ-TuRC–mediated nucleation activator (γ-TuNA). γ-TuNA but not its γ-TuC–binding-deficient mutant stimulates microtubule nucleation by purified γ-TuRC in vitro and induces extensive, γ-TuRC-dependent nucleation of microtubules in a microtubule regrowth assay. γ-TuRC bound to γ-TuNA contains NME7, FAM128A/B, and actin in addition to γ-tubulin and GCP2–6. RNA interference–mediated depletion of CDK5RAP2 impairs both centrosomal and acentrosomal microtubule nucleation, although γ-TuRC assembly is unaffected. Collectively, these results suggest that the γ-TuNA found in CDK5RAP2 has regulatory functions in γ-TuRC–mediated microtubule nucleation. The Rockefeller University Press 2010-12-13 /pmc/articles/PMC3002024/ /pubmed/21135143 http://dx.doi.org/10.1083/jcb.201007030 Text en © 2010 Choi et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Choi, Yuk-Kwan
Liu, Pengfei
Sze, Siu Kwan
Dai, Chao
Qi, Robert Z.
CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex
title CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex
title_full CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex
title_fullStr CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex
title_full_unstemmed CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex
title_short CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex
title_sort cdk5rap2 stimulates microtubule nucleation by the γ-tubulin ring complex
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002024/
https://www.ncbi.nlm.nih.gov/pubmed/21135143
http://dx.doi.org/10.1083/jcb.201007030
work_keys_str_mv AT choiyukkwan cdk5rap2stimulatesmicrotubulenucleationbythegtubulinringcomplex
AT liupengfei cdk5rap2stimulatesmicrotubulenucleationbythegtubulinringcomplex
AT szesiukwan cdk5rap2stimulatesmicrotubulenucleationbythegtubulinringcomplex
AT daichao cdk5rap2stimulatesmicrotubulenucleationbythegtubulinringcomplex
AT qirobertz cdk5rap2stimulatesmicrotubulenucleationbythegtubulinringcomplex