Cargando…
CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex
CDK5RAP2 is a human microcephaly protein that contains a γ-tubulin complex (γ-TuC)–binding domain conserved in Drosophila melanogaster centrosomin and Schizosaccharomyces pombe Mto1p and Pcp1p, which are γ-TuC–tethering proteins. In this study, we show that this domain within CDK5RAP2 associates wit...
Autores principales: | , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002024/ https://www.ncbi.nlm.nih.gov/pubmed/21135143 http://dx.doi.org/10.1083/jcb.201007030 |
_version_ | 1782193686305570816 |
---|---|
author | Choi, Yuk-Kwan Liu, Pengfei Sze, Siu Kwan Dai, Chao Qi, Robert Z. |
author_facet | Choi, Yuk-Kwan Liu, Pengfei Sze, Siu Kwan Dai, Chao Qi, Robert Z. |
author_sort | Choi, Yuk-Kwan |
collection | PubMed |
description | CDK5RAP2 is a human microcephaly protein that contains a γ-tubulin complex (γ-TuC)–binding domain conserved in Drosophila melanogaster centrosomin and Schizosaccharomyces pombe Mto1p and Pcp1p, which are γ-TuC–tethering proteins. In this study, we show that this domain within CDK5RAP2 associates with the γ-tubulin ring complex (γ-TuRC) to stimulate its microtubule-nucleating activity and is therefore referred to as the γ-TuRC–mediated nucleation activator (γ-TuNA). γ-TuNA but not its γ-TuC–binding-deficient mutant stimulates microtubule nucleation by purified γ-TuRC in vitro and induces extensive, γ-TuRC-dependent nucleation of microtubules in a microtubule regrowth assay. γ-TuRC bound to γ-TuNA contains NME7, FAM128A/B, and actin in addition to γ-tubulin and GCP2–6. RNA interference–mediated depletion of CDK5RAP2 impairs both centrosomal and acentrosomal microtubule nucleation, although γ-TuRC assembly is unaffected. Collectively, these results suggest that the γ-TuNA found in CDK5RAP2 has regulatory functions in γ-TuRC–mediated microtubule nucleation. |
format | Text |
id | pubmed-3002024 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-30020242011-06-13 CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex Choi, Yuk-Kwan Liu, Pengfei Sze, Siu Kwan Dai, Chao Qi, Robert Z. J Cell Biol Research Articles CDK5RAP2 is a human microcephaly protein that contains a γ-tubulin complex (γ-TuC)–binding domain conserved in Drosophila melanogaster centrosomin and Schizosaccharomyces pombe Mto1p and Pcp1p, which are γ-TuC–tethering proteins. In this study, we show that this domain within CDK5RAP2 associates with the γ-tubulin ring complex (γ-TuRC) to stimulate its microtubule-nucleating activity and is therefore referred to as the γ-TuRC–mediated nucleation activator (γ-TuNA). γ-TuNA but not its γ-TuC–binding-deficient mutant stimulates microtubule nucleation by purified γ-TuRC in vitro and induces extensive, γ-TuRC-dependent nucleation of microtubules in a microtubule regrowth assay. γ-TuRC bound to γ-TuNA contains NME7, FAM128A/B, and actin in addition to γ-tubulin and GCP2–6. RNA interference–mediated depletion of CDK5RAP2 impairs both centrosomal and acentrosomal microtubule nucleation, although γ-TuRC assembly is unaffected. Collectively, these results suggest that the γ-TuNA found in CDK5RAP2 has regulatory functions in γ-TuRC–mediated microtubule nucleation. The Rockefeller University Press 2010-12-13 /pmc/articles/PMC3002024/ /pubmed/21135143 http://dx.doi.org/10.1083/jcb.201007030 Text en © 2010 Choi et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Choi, Yuk-Kwan Liu, Pengfei Sze, Siu Kwan Dai, Chao Qi, Robert Z. CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex |
title | CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex |
title_full | CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex |
title_fullStr | CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex |
title_full_unstemmed | CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex |
title_short | CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex |
title_sort | cdk5rap2 stimulates microtubule nucleation by the γ-tubulin ring complex |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002024/ https://www.ncbi.nlm.nih.gov/pubmed/21135143 http://dx.doi.org/10.1083/jcb.201007030 |
work_keys_str_mv | AT choiyukkwan cdk5rap2stimulatesmicrotubulenucleationbythegtubulinringcomplex AT liupengfei cdk5rap2stimulatesmicrotubulenucleationbythegtubulinringcomplex AT szesiukwan cdk5rap2stimulatesmicrotubulenucleationbythegtubulinringcomplex AT daichao cdk5rap2stimulatesmicrotubulenucleationbythegtubulinringcomplex AT qirobertz cdk5rap2stimulatesmicrotubulenucleationbythegtubulinringcomplex |