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Multiple loci contribute to genome-wide recombination levels in male mice
Recent linkage-based studies in humans suggest the presence of loci that affect either genome-wide recombination rates, utilization of recombination hotspots, or both. We have been interested in utilizing cytological methodology to directly assess recombination in mammalian meiocytes and to identify...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002158/ https://www.ncbi.nlm.nih.gov/pubmed/21113599 http://dx.doi.org/10.1007/s00335-010-9303-5 |
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author | Murdoch, Brenda Owen, Nichole Shirley, Sofia Crumb, Sara Broman, Karl W. Hassold, Terry |
author_facet | Murdoch, Brenda Owen, Nichole Shirley, Sofia Crumb, Sara Broman, Karl W. Hassold, Terry |
author_sort | Murdoch, Brenda |
collection | PubMed |
description | Recent linkage-based studies in humans suggest the presence of loci that affect either genome-wide recombination rates, utilization of recombination hotspots, or both. We have been interested in utilizing cytological methodology to directly assess recombination in mammalian meiocytes and to identify recombination-associated loci. In the present report we summarize studies in which we combined a cytological assay of recombination in mouse pachytene spermatocytes with QTL analyses to identify loci that contribute to genome-wide levels of recombination in male meiosis. Specifically, we analyzed MLH1 foci, a marker of crossovers, in 194 F2 male mice derived from a subspecific cross between CAST/EiJ and C57BL/6J parental strains. We then used these data to uncover loci associated with individual variation in mean MLH1 values. We identified seven recombination-associated loci across the genome (on chromosomes 2, 3, 4, 14, 15, 17, and X), indicating that there are multiple recombination “setting” loci in mammalian male meiosis. |
format | Text |
id | pubmed-3002158 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-30021582011-01-19 Multiple loci contribute to genome-wide recombination levels in male mice Murdoch, Brenda Owen, Nichole Shirley, Sofia Crumb, Sara Broman, Karl W. Hassold, Terry Mamm Genome Article Recent linkage-based studies in humans suggest the presence of loci that affect either genome-wide recombination rates, utilization of recombination hotspots, or both. We have been interested in utilizing cytological methodology to directly assess recombination in mammalian meiocytes and to identify recombination-associated loci. In the present report we summarize studies in which we combined a cytological assay of recombination in mouse pachytene spermatocytes with QTL analyses to identify loci that contribute to genome-wide levels of recombination in male meiosis. Specifically, we analyzed MLH1 foci, a marker of crossovers, in 194 F2 male mice derived from a subspecific cross between CAST/EiJ and C57BL/6J parental strains. We then used these data to uncover loci associated with individual variation in mean MLH1 values. We identified seven recombination-associated loci across the genome (on chromosomes 2, 3, 4, 14, 15, 17, and X), indicating that there are multiple recombination “setting” loci in mammalian male meiosis. Springer-Verlag 2010-11-27 2010 /pmc/articles/PMC3002158/ /pubmed/21113599 http://dx.doi.org/10.1007/s00335-010-9303-5 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Article Murdoch, Brenda Owen, Nichole Shirley, Sofia Crumb, Sara Broman, Karl W. Hassold, Terry Multiple loci contribute to genome-wide recombination levels in male mice |
title | Multiple loci contribute to genome-wide recombination levels in male mice |
title_full | Multiple loci contribute to genome-wide recombination levels in male mice |
title_fullStr | Multiple loci contribute to genome-wide recombination levels in male mice |
title_full_unstemmed | Multiple loci contribute to genome-wide recombination levels in male mice |
title_short | Multiple loci contribute to genome-wide recombination levels in male mice |
title_sort | multiple loci contribute to genome-wide recombination levels in male mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002158/ https://www.ncbi.nlm.nih.gov/pubmed/21113599 http://dx.doi.org/10.1007/s00335-010-9303-5 |
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