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Identification and isolation of Genotype-I Japanese Encephalitis virus from encephalitis patients

Historically, Japanese Encephalitis virus (JEV) genotype III (GIII) has been responsible for human diseases. In recent years, JEV genotype I (GI) has been isolated from mosquitoes collected in numerous countries, but has not been isolated from patients with encephalitis. In this study, we report rec...

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Autores principales: Wang, Lihua, Fu, Shihong, Zhang, Hailin, Ye, Xufang, Yu, Deshan, Deng, Zhang, Yuan, Jun, Zhai, Yougang, Li, Minghua, Lv, Zhi, Chen, Weixin, Jiang, Hongyue, Gao, Xiaoyan, Cao, Yuxi, Wang, Huanyu, Tang, Qing, Liang, Guodong
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002311/
https://www.ncbi.nlm.nih.gov/pubmed/21108846
http://dx.doi.org/10.1186/1743-422X-7-345
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author Wang, Lihua
Fu, Shihong
Zhang, Hailin
Ye, Xufang
Yu, Deshan
Deng, Zhang
Yuan, Jun
Zhai, Yougang
Li, Minghua
Lv, Zhi
Chen, Weixin
Jiang, Hongyue
Gao, Xiaoyan
Cao, Yuxi
Wang, Huanyu
Tang, Qing
Liang, Guodong
author_facet Wang, Lihua
Fu, Shihong
Zhang, Hailin
Ye, Xufang
Yu, Deshan
Deng, Zhang
Yuan, Jun
Zhai, Yougang
Li, Minghua
Lv, Zhi
Chen, Weixin
Jiang, Hongyue
Gao, Xiaoyan
Cao, Yuxi
Wang, Huanyu
Tang, Qing
Liang, Guodong
author_sort Wang, Lihua
collection PubMed
description Historically, Japanese Encephalitis virus (JEV) genotype III (GIII) has been responsible for human diseases. In recent years, JEV genotype I (GI) has been isolated from mosquitoes collected in numerous countries, but has not been isolated from patients with encephalitis. In this study, we report recovery of JEV GI live virus and identification of JEV GI RNA from cerebrospinal fluid (CSF) of encephalitis patients in JE endemic areas of China. Whole-genome sequencing and molecular phylogenetic analysis of the JEV isolate from the CSF samples was performed. The isolate in this study is highly similar to other JEV GI strains which isolated from mosquitoes at both the nucleotide and deduced amino acid levels. Phylogenetic analysis based on the genomic sequence showed that the isolate belongs to JEV GI, which is consistent with the phylogenetic analysis based on the pre-membrane (PrM) and Glycoprotein genes. As a conclusion, this is the first time to isolate JEV GI strain from CSF samples of encephalitis patients, so continuous survey and evaluate the infectivity and pathogenecity of JEV GI strains are necessary, especially for the JEV GI strains from encephalitis patients. With respect to the latter, because all current JEV vaccines (live and inactivated are derived from JEV GIII strains, future studies should be aimed at investigating and monitoring cross-protection of the human JEV GI isolates against widely used JEV vaccines.
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spelling pubmed-30023112010-12-16 Identification and isolation of Genotype-I Japanese Encephalitis virus from encephalitis patients Wang, Lihua Fu, Shihong Zhang, Hailin Ye, Xufang Yu, Deshan Deng, Zhang Yuan, Jun Zhai, Yougang Li, Minghua Lv, Zhi Chen, Weixin Jiang, Hongyue Gao, Xiaoyan Cao, Yuxi Wang, Huanyu Tang, Qing Liang, Guodong Virol J Short Report Historically, Japanese Encephalitis virus (JEV) genotype III (GIII) has been responsible for human diseases. In recent years, JEV genotype I (GI) has been isolated from mosquitoes collected in numerous countries, but has not been isolated from patients with encephalitis. In this study, we report recovery of JEV GI live virus and identification of JEV GI RNA from cerebrospinal fluid (CSF) of encephalitis patients in JE endemic areas of China. Whole-genome sequencing and molecular phylogenetic analysis of the JEV isolate from the CSF samples was performed. The isolate in this study is highly similar to other JEV GI strains which isolated from mosquitoes at both the nucleotide and deduced amino acid levels. Phylogenetic analysis based on the genomic sequence showed that the isolate belongs to JEV GI, which is consistent with the phylogenetic analysis based on the pre-membrane (PrM) and Glycoprotein genes. As a conclusion, this is the first time to isolate JEV GI strain from CSF samples of encephalitis patients, so continuous survey and evaluate the infectivity and pathogenecity of JEV GI strains are necessary, especially for the JEV GI strains from encephalitis patients. With respect to the latter, because all current JEV vaccines (live and inactivated are derived from JEV GIII strains, future studies should be aimed at investigating and monitoring cross-protection of the human JEV GI isolates against widely used JEV vaccines. BioMed Central 2010-11-26 /pmc/articles/PMC3002311/ /pubmed/21108846 http://dx.doi.org/10.1186/1743-422X-7-345 Text en Copyright ©2010 Wang et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Wang, Lihua
Fu, Shihong
Zhang, Hailin
Ye, Xufang
Yu, Deshan
Deng, Zhang
Yuan, Jun
Zhai, Yougang
Li, Minghua
Lv, Zhi
Chen, Weixin
Jiang, Hongyue
Gao, Xiaoyan
Cao, Yuxi
Wang, Huanyu
Tang, Qing
Liang, Guodong
Identification and isolation of Genotype-I Japanese Encephalitis virus from encephalitis patients
title Identification and isolation of Genotype-I Japanese Encephalitis virus from encephalitis patients
title_full Identification and isolation of Genotype-I Japanese Encephalitis virus from encephalitis patients
title_fullStr Identification and isolation of Genotype-I Japanese Encephalitis virus from encephalitis patients
title_full_unstemmed Identification and isolation of Genotype-I Japanese Encephalitis virus from encephalitis patients
title_short Identification and isolation of Genotype-I Japanese Encephalitis virus from encephalitis patients
title_sort identification and isolation of genotype-i japanese encephalitis virus from encephalitis patients
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002311/
https://www.ncbi.nlm.nih.gov/pubmed/21108846
http://dx.doi.org/10.1186/1743-422X-7-345
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