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Expression of the chemokine receptor CXCR4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus

BACKGROUND: This study was conducted to investigate the expression of CXCR4 in portal vein tumor thrombus (PVTT) tissue and its possible role in the invasiveness of tumor thrombus cells. METHODS: We detected differential expression of CXCR4 between PVTT and hepatocellular carcinoma (HCC) by an immun...

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Autores principales: Li, Nan, Guo, Weixing, Shi, Jie, Xue, Jie, Hu, Huasheng, Xie, Dong, Wu, Mengchao, Cheng, Shuqun
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002328/
https://www.ncbi.nlm.nih.gov/pubmed/21110890
http://dx.doi.org/10.1186/1756-9966-29-156
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author Li, Nan
Guo, Weixing
Shi, Jie
Xue, Jie
Hu, Huasheng
Xie, Dong
Wu, Mengchao
Cheng, Shuqun
author_facet Li, Nan
Guo, Weixing
Shi, Jie
Xue, Jie
Hu, Huasheng
Xie, Dong
Wu, Mengchao
Cheng, Shuqun
author_sort Li, Nan
collection PubMed
description BACKGROUND: This study was conducted to investigate the expression of CXCR4 in portal vein tumor thrombus (PVTT) tissue and its possible role in the invasiveness of tumor thrombus cells. METHODS: We detected differential expression of CXCR4 between PVTT and hepatocellular carcinoma (HCC) by an immunohistochemical assay. Lentivirus-mediated RNA interference and a migration assay were performed on human primary cells derived from PVTT to study the impact of CXCR4 on the invasiveness of HCC. RESULTS: The expression of CXCR4 in tumor thrombus tissue was higher than that in HCC tissue. The invasion ratio of PVTT cells was significantly decreased (P < 0.05) after being infected with a CXCR4-targeting siRNA lentivirus, indicating that downregulation of CXCR4 by lentivirus-mediated RNA interference significantly impaired the invasive potential of PVTT. CONCLUSIONS: These results indicate that CXCR4 is an effective curative target for hepatocellular carcinomas with PVTT.
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spelling pubmed-30023282010-12-16 Expression of the chemokine receptor CXCR4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus Li, Nan Guo, Weixing Shi, Jie Xue, Jie Hu, Huasheng Xie, Dong Wu, Mengchao Cheng, Shuqun J Exp Clin Cancer Res Research BACKGROUND: This study was conducted to investigate the expression of CXCR4 in portal vein tumor thrombus (PVTT) tissue and its possible role in the invasiveness of tumor thrombus cells. METHODS: We detected differential expression of CXCR4 between PVTT and hepatocellular carcinoma (HCC) by an immunohistochemical assay. Lentivirus-mediated RNA interference and a migration assay were performed on human primary cells derived from PVTT to study the impact of CXCR4 on the invasiveness of HCC. RESULTS: The expression of CXCR4 in tumor thrombus tissue was higher than that in HCC tissue. The invasion ratio of PVTT cells was significantly decreased (P < 0.05) after being infected with a CXCR4-targeting siRNA lentivirus, indicating that downregulation of CXCR4 by lentivirus-mediated RNA interference significantly impaired the invasive potential of PVTT. CONCLUSIONS: These results indicate that CXCR4 is an effective curative target for hepatocellular carcinomas with PVTT. BioMed Central 2010-11-27 /pmc/articles/PMC3002328/ /pubmed/21110890 http://dx.doi.org/10.1186/1756-9966-29-156 Text en Copyright ©2010 Li et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Li, Nan
Guo, Weixing
Shi, Jie
Xue, Jie
Hu, Huasheng
Xie, Dong
Wu, Mengchao
Cheng, Shuqun
Expression of the chemokine receptor CXCR4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus
title Expression of the chemokine receptor CXCR4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus
title_full Expression of the chemokine receptor CXCR4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus
title_fullStr Expression of the chemokine receptor CXCR4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus
title_full_unstemmed Expression of the chemokine receptor CXCR4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus
title_short Expression of the chemokine receptor CXCR4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus
title_sort expression of the chemokine receptor cxcr4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002328/
https://www.ncbi.nlm.nih.gov/pubmed/21110890
http://dx.doi.org/10.1186/1756-9966-29-156
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