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Expression of the chemokine receptor CXCR4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus
BACKGROUND: This study was conducted to investigate the expression of CXCR4 in portal vein tumor thrombus (PVTT) tissue and its possible role in the invasiveness of tumor thrombus cells. METHODS: We detected differential expression of CXCR4 between PVTT and hepatocellular carcinoma (HCC) by an immun...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002328/ https://www.ncbi.nlm.nih.gov/pubmed/21110890 http://dx.doi.org/10.1186/1756-9966-29-156 |
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author | Li, Nan Guo, Weixing Shi, Jie Xue, Jie Hu, Huasheng Xie, Dong Wu, Mengchao Cheng, Shuqun |
author_facet | Li, Nan Guo, Weixing Shi, Jie Xue, Jie Hu, Huasheng Xie, Dong Wu, Mengchao Cheng, Shuqun |
author_sort | Li, Nan |
collection | PubMed |
description | BACKGROUND: This study was conducted to investigate the expression of CXCR4 in portal vein tumor thrombus (PVTT) tissue and its possible role in the invasiveness of tumor thrombus cells. METHODS: We detected differential expression of CXCR4 between PVTT and hepatocellular carcinoma (HCC) by an immunohistochemical assay. Lentivirus-mediated RNA interference and a migration assay were performed on human primary cells derived from PVTT to study the impact of CXCR4 on the invasiveness of HCC. RESULTS: The expression of CXCR4 in tumor thrombus tissue was higher than that in HCC tissue. The invasion ratio of PVTT cells was significantly decreased (P < 0.05) after being infected with a CXCR4-targeting siRNA lentivirus, indicating that downregulation of CXCR4 by lentivirus-mediated RNA interference significantly impaired the invasive potential of PVTT. CONCLUSIONS: These results indicate that CXCR4 is an effective curative target for hepatocellular carcinomas with PVTT. |
format | Text |
id | pubmed-3002328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30023282010-12-16 Expression of the chemokine receptor CXCR4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus Li, Nan Guo, Weixing Shi, Jie Xue, Jie Hu, Huasheng Xie, Dong Wu, Mengchao Cheng, Shuqun J Exp Clin Cancer Res Research BACKGROUND: This study was conducted to investigate the expression of CXCR4 in portal vein tumor thrombus (PVTT) tissue and its possible role in the invasiveness of tumor thrombus cells. METHODS: We detected differential expression of CXCR4 between PVTT and hepatocellular carcinoma (HCC) by an immunohistochemical assay. Lentivirus-mediated RNA interference and a migration assay were performed on human primary cells derived from PVTT to study the impact of CXCR4 on the invasiveness of HCC. RESULTS: The expression of CXCR4 in tumor thrombus tissue was higher than that in HCC tissue. The invasion ratio of PVTT cells was significantly decreased (P < 0.05) after being infected with a CXCR4-targeting siRNA lentivirus, indicating that downregulation of CXCR4 by lentivirus-mediated RNA interference significantly impaired the invasive potential of PVTT. CONCLUSIONS: These results indicate that CXCR4 is an effective curative target for hepatocellular carcinomas with PVTT. BioMed Central 2010-11-27 /pmc/articles/PMC3002328/ /pubmed/21110890 http://dx.doi.org/10.1186/1756-9966-29-156 Text en Copyright ©2010 Li et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Li, Nan Guo, Weixing Shi, Jie Xue, Jie Hu, Huasheng Xie, Dong Wu, Mengchao Cheng, Shuqun Expression of the chemokine receptor CXCR4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus |
title | Expression of the chemokine receptor CXCR4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus |
title_full | Expression of the chemokine receptor CXCR4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus |
title_fullStr | Expression of the chemokine receptor CXCR4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus |
title_full_unstemmed | Expression of the chemokine receptor CXCR4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus |
title_short | Expression of the chemokine receptor CXCR4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus |
title_sort | expression of the chemokine receptor cxcr4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002328/ https://www.ncbi.nlm.nih.gov/pubmed/21110890 http://dx.doi.org/10.1186/1756-9966-29-156 |
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