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Preparation and In Vivo Evaluation of Indomethacin Loaded True Nanoemulsions

Indomethacin, a potent nonsteroidal anti-inflammatory drug, has been used in the treatment of various kinds of pains, inflammation and arthritis. However, oral administration of indomethacin produces serious gastrointestinal adverse effects. Therefore the aim of the present investigation was to eval...

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Autores principales: Shakeel, Faiyaz, Ramadan, Wafa, Gargum, Huda M., Singh, Rajinder
Formato: Texto
Lenguaje:English
Publicado: Österreichische Apotheker-Verlagsgesellschaft 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002824/
https://www.ncbi.nlm.nih.gov/pubmed/21179369
http://dx.doi.org/10.3797/scipharm.0911-04
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author Shakeel, Faiyaz
Ramadan, Wafa
Gargum, Huda M.
Singh, Rajinder
author_facet Shakeel, Faiyaz
Ramadan, Wafa
Gargum, Huda M.
Singh, Rajinder
author_sort Shakeel, Faiyaz
collection PubMed
description Indomethacin, a potent nonsteroidal anti-inflammatory drug, has been used in the treatment of various kinds of pains, inflammation and arthritis. However, oral administration of indomethacin produces serious gastrointestinal adverse effects. Therefore the aim of the present investigation was to evaluate the anti-inflammatory effects, skin irritation, activation energy and histopathology of indomethacin from transdermally applied true nanoemulsion. The anti-inflammatory effects of true nanoemulsions were compared with marketed Indobene(®) gel on carrageenan-induced paw edema in rats. Skin irritation tests were performed on Wistar rats for 14 days. The % inhibition value after 12 h application was significant for optimized formulation F6 (83) as compared to marketed Indobene(®) gel (P<0.01). Results of skin irritation test indicated that developed true nanoemulsion is safe for human use. The significant decrease in activation energy (1.396 kcal/mol) for indomethacin across rat skin indicated that the stratum corneum lipid bilayers were significantly disrupted (P<0.05). From these results it was concluded that the developed nanoemulsion have great potential for transdermal application of indomethacin.
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spelling pubmed-30028242010-12-22 Preparation and In Vivo Evaluation of Indomethacin Loaded True Nanoemulsions Shakeel, Faiyaz Ramadan, Wafa Gargum, Huda M. Singh, Rajinder Sci Pharm Original Papers Indomethacin, a potent nonsteroidal anti-inflammatory drug, has been used in the treatment of various kinds of pains, inflammation and arthritis. However, oral administration of indomethacin produces serious gastrointestinal adverse effects. Therefore the aim of the present investigation was to evaluate the anti-inflammatory effects, skin irritation, activation energy and histopathology of indomethacin from transdermally applied true nanoemulsion. The anti-inflammatory effects of true nanoemulsions were compared with marketed Indobene(®) gel on carrageenan-induced paw edema in rats. Skin irritation tests were performed on Wistar rats for 14 days. The % inhibition value after 12 h application was significant for optimized formulation F6 (83) as compared to marketed Indobene(®) gel (P<0.01). Results of skin irritation test indicated that developed true nanoemulsion is safe for human use. The significant decrease in activation energy (1.396 kcal/mol) for indomethacin across rat skin indicated that the stratum corneum lipid bilayers were significantly disrupted (P<0.05). From these results it was concluded that the developed nanoemulsion have great potential for transdermal application of indomethacin. Österreichische Apotheker-Verlagsgesellschaft 2010 2009-11-23 /pmc/articles/PMC3002824/ /pubmed/21179369 http://dx.doi.org/10.3797/scipharm.0911-04 Text en © Shakeel et al.; licensee Österreichische Apotheker-Verlagsgesellschaft m. b. H., Vienna, Austria. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Papers
Shakeel, Faiyaz
Ramadan, Wafa
Gargum, Huda M.
Singh, Rajinder
Preparation and In Vivo Evaluation of Indomethacin Loaded True Nanoemulsions
title Preparation and In Vivo Evaluation of Indomethacin Loaded True Nanoemulsions
title_full Preparation and In Vivo Evaluation of Indomethacin Loaded True Nanoemulsions
title_fullStr Preparation and In Vivo Evaluation of Indomethacin Loaded True Nanoemulsions
title_full_unstemmed Preparation and In Vivo Evaluation of Indomethacin Loaded True Nanoemulsions
title_short Preparation and In Vivo Evaluation of Indomethacin Loaded True Nanoemulsions
title_sort preparation and in vivo evaluation of indomethacin loaded true nanoemulsions
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002824/
https://www.ncbi.nlm.nih.gov/pubmed/21179369
http://dx.doi.org/10.3797/scipharm.0911-04
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