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Antiproliferative Activity of Plant Extracts Used Against Cancer in Traditional Medicine

Forty four extracts from sixteen plants used traditionally as anticancer agents were evaluated in vitro for their antiproliferative activity against Hep-2, MCF-7, and Vero cell lines. Plants were fractionated using ethanol, methanol, chloroform, n-hexane, distilled water, and butanol. The antiprolif...

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Autores principales: Talib, Wamidh H., Mahasneh, Adel M.
Formato: Texto
Lenguaje:English
Publicado: Österreichische Apotheker-Verlagsgesellschaft 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002826/
https://www.ncbi.nlm.nih.gov/pubmed/21179373
http://dx.doi.org/10.3797/scipharm.0912-11
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author Talib, Wamidh H.
Mahasneh, Adel M.
author_facet Talib, Wamidh H.
Mahasneh, Adel M.
author_sort Talib, Wamidh H.
collection PubMed
description Forty four extracts from sixteen plants used traditionally as anticancer agents were evaluated in vitro for their antiproliferative activity against Hep-2, MCF-7, and Vero cell lines. Plants were fractionated using ethanol, methanol, chloroform, n-hexane, distilled water, and butanol. The antiproliferative activity was measured by MTT assay. TLC was used to identify active fractions. The apoptotic activity of active fractions was determined using TUNEL colorimetric assay. 20 of these extracts demonstrated significant antiproliferative activity against one or more of the cell lines. These extracts were prepared from Ononis hirta, Inula viscosa, Salvia pinardi, Verbascum sinaiticum and Ononis sicula. Methanol fractions of Ononis hirta (aerial parts) and Inula viscosa (flowers) were the most active fractions against MCF-7 cells with IC(50) of 27.96 and 15.78 μg/ml respectively and they were less toxic against other cell lines. Other extracts showed lower activity against cancer cell lines. TLC analysis showed the presence of flavonoids and terpenoids in active plants while alkaloids were detected in Ononis hirta (aerial parts) extracts. Ononis hirta (aerial parts) and Inula viscosa (flowers) extracts exerted their antiproliferative activity by inducing apoptosis in cancer cell lines. Further studies are necessary for detailed chemical characterization and more extensive biological evaluation of the most active ingredients.
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spelling pubmed-30028262010-12-22 Antiproliferative Activity of Plant Extracts Used Against Cancer in Traditional Medicine Talib, Wamidh H. Mahasneh, Adel M. Sci Pharm Original Papers Forty four extracts from sixteen plants used traditionally as anticancer agents were evaluated in vitro for their antiproliferative activity against Hep-2, MCF-7, and Vero cell lines. Plants were fractionated using ethanol, methanol, chloroform, n-hexane, distilled water, and butanol. The antiproliferative activity was measured by MTT assay. TLC was used to identify active fractions. The apoptotic activity of active fractions was determined using TUNEL colorimetric assay. 20 of these extracts demonstrated significant antiproliferative activity against one or more of the cell lines. These extracts were prepared from Ononis hirta, Inula viscosa, Salvia pinardi, Verbascum sinaiticum and Ononis sicula. Methanol fractions of Ononis hirta (aerial parts) and Inula viscosa (flowers) were the most active fractions against MCF-7 cells with IC(50) of 27.96 and 15.78 μg/ml respectively and they were less toxic against other cell lines. Other extracts showed lower activity against cancer cell lines. TLC analysis showed the presence of flavonoids and terpenoids in active plants while alkaloids were detected in Ononis hirta (aerial parts) extracts. Ononis hirta (aerial parts) and Inula viscosa (flowers) extracts exerted their antiproliferative activity by inducing apoptosis in cancer cell lines. Further studies are necessary for detailed chemical characterization and more extensive biological evaluation of the most active ingredients. Österreichische Apotheker-Verlagsgesellschaft 2010 2010-02-13 /pmc/articles/PMC3002826/ /pubmed/21179373 http://dx.doi.org/10.3797/scipharm.0912-11 Text en © Talib and Mahasneh; licensee Österreichische Apotheker-Verlagsgesellschaft m. b. H., Vienna, Austria. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Papers
Talib, Wamidh H.
Mahasneh, Adel M.
Antiproliferative Activity of Plant Extracts Used Against Cancer in Traditional Medicine
title Antiproliferative Activity of Plant Extracts Used Against Cancer in Traditional Medicine
title_full Antiproliferative Activity of Plant Extracts Used Against Cancer in Traditional Medicine
title_fullStr Antiproliferative Activity of Plant Extracts Used Against Cancer in Traditional Medicine
title_full_unstemmed Antiproliferative Activity of Plant Extracts Used Against Cancer in Traditional Medicine
title_short Antiproliferative Activity of Plant Extracts Used Against Cancer in Traditional Medicine
title_sort antiproliferative activity of plant extracts used against cancer in traditional medicine
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002826/
https://www.ncbi.nlm.nih.gov/pubmed/21179373
http://dx.doi.org/10.3797/scipharm.0912-11
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